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Molecular and Cellular Biology, January 2001, p. 476-487, Vol. 21, No. 2
The Wistar Institute1
and Division of Hematology, The Children's Hospital of
Philadelphia and the University of Pennsylvania School of
Medicine,2 Philadelphia, Pennsylvania 19104
Received 31 March 2000/Returned for modification 15 September
2000/Accepted 27 October 2000
The transcriptional coactivator CREB binding protein (CBP)
possesses intrinsic histone acetyltransferase (HAT) activity that is
important for gene regulation. CBP binds to and cooperates with
numerous nuclear factors to stimulate transcription, but it is unclear
if these factors modulate CBP HAT activity. Our previous work showed
that CBP interacts with the Epstein-Barr virus-encoded basic region
zipper (b-zip) protein, Zta, and augments its transcriptional activity.
Here we report that Zta strongly enhances CBP-mediated acetylation of
nucleosomal histones. Zta stimulated the HAT activity of CBP that had
been partially purified or immunoprecipitated from mammalian cells as
well as from affinity-purified, baculovirus expressed CBP. Stimulation
of nucleosome acetylation required the CBP HAT domain, the Zta DNA
binding and transcription activation domain, and nucleosomal DNA. In
addition to Zta, we found that two other b-zip proteins, NF-E2 and
C/EBP
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.2.476-487.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Stimulation of CREB Binding Protein Nucleosomal
Histone Acetyltransferase Activity by a Class of Transcriptional
Activators
, strongly stimulated nucleosomal HAT activity. In contrast,
several CBP-binding proteins, including phospho-CREB, JUN/FOS, GATA-1,
Pit-1, and EKLF, failed to stimulate HAT activity. These results
demonstrate that a subset of transcriptional activators enhance the
nucleosome-directed HAT activity of CBP and suggest that nuclear
factors may regulate transcription by altering substrate recognition
and/or the enzymatic activity of chromatin modifying coactivators.
*
Corresponding author. Mailing address: The Wistar
Institute, 3601 Spruce St., Philadelphia, PA 19104-4268. Phone: (215)
898-9491. Fax: (215) 898-0663. E-mail:
lieberman{at}wista.wistar.upenn.edu.
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