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Molecular and Cellular Biology, January 2001, p. 636-643, Vol. 21, No. 2
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.2.636-643.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mutation and Analysis of Dan, the Founding Member of the Dan Family of Transforming Growth Factor beta  Antagonists

Marc S. Dionne, William C. Skarnes, and Richard M. Harland*

Division of Genetics and Development, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720

Received 13 October 2000/Accepted 18 October 2000

The Dan family of transforming growth factor beta  antagonists is a large, evolutionarily conserved family of proteins. Little is known about either the specificity of these antagonists or the biological roles of these proteins. We have characterized Dan, the founding member of this family, with regard to both its biochemical specificity and its biological roles. Although DAN is not an efficient antagonist of BMP-2/4 class signals, we found that DAN was able to interact with GDF-5 in a frog embryo assay, suggesting that DAN may regulate signaling by the GDF-5/6/7 class of BMPs in vivo. Intriguingly, in developing neurons, Dan mRNA was localized to axons, suggesting a potential role for the DAN protein in axonal outgrowth or guidance. Mice lacking Dan activity were generated by gene targeting and displayed subtle, background-dependent defects.

* Corresponding author. Mailing address: 401 Barker Hall, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3202. Phone: (510) 643-6003. Fax: (510) 643-1729. E-mail: harland{at}socrates.berkeley.edu.

Molecular and Cellular Biology, January 2001, p. 636-643, Vol. 21, No. 2
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.2.636-643.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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