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Molecular and Cellular Biology, October 2001, p. 6833-6840, Vol. 21, No. 20
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.20.6833-6840.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Four Related Proteins of the Trypanosoma
brucei RNA Editing Complex
Aswini K.
Panigrahi,1,2
Achim
Schnaufer,1,2
Nicole
Carmean,1
Robert P.
Igo Jr.,1,2
Steven P.
Gygi,3,
Nancy L.
Ernst,1,2
Setareh S.
Palazzo,1,2
David S.
Weston,1,2,
Ruedi
Aebersold,3,4
Reza
Salavati,1,2 and
Kenneth D.
Stuart1,2,*
Seattle Biomedical Research Institute, Seattle, Washington
981091; Departments of
Pathobiology2 and Molecular
Biotechnology,3 University of Washington,
Seattle, Washington 98195; and The Institute for Systems
Biology, Seattle, Washington 981054
Received 16 May 2001/Returned for modification 28 June
2001/Accepted 16 July 2001
RNA editing in kinetoplastid mitochondria occurs by a series of
enzymatic steps that is catalyzed by a macromolecular complex. Four
novel proteins and their corresponding genes were identified by mass
spectrometric analysis of purified editing complexes from Trypanosoma brucei. These four proteins, TbMP81, TbMP63,
TbMP42, and TbMP18, contain conserved sequences to various degrees. All four proteins have sequence similarity in the C terminus; TbMP18 has
considerable sequence similarity to the C-terminal region of TbMP42,
and TbMP81, TbMP63, and TbMP42 contain zinc finger motif(s). Monoclonal
antibodies that are specific for TbMP63 and TbMP42 immunoprecipitate in
vitro RNA editing activities. The proteins are present in the
immunoprecipitates and sediment at 20S along with the in vitro editing,
and RNA editing ligases TbMP52 and TbMP48. Recombinant TbMP63 and
TbMP52 coimmunoprecipitate. These results indicate that these four
proteins are components of the RNA editing complex and that TbMP63 and
TbMP52 can interact.
*
Corresponding author. Mailing address: Seattle
Biomedical Research Institute, 4 Nickerson St., Seattle, WA 98109. Phone: (206) 284-8846, ext. 316. Fax: (206) 284-0313. E-mail:
kstuart{at}u.washington.edu.

Present address: Department of Cell Biology, Harvard Medical
School, Boston, MA
02115.

Present address: CompleGen Inc., Seattle, WA
98104.
Molecular and Cellular Biology, October 2001, p. 6833-6840, Vol. 21, No. 20
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.20.6833-6840.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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