This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mukhopadhyay, S. S.
Right arrow Articles by Rosen, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mukhopadhyay, S. S.
Right arrow Articles by Rosen, J. M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2001, p. 6859-6869, Vol. 21, No. 20
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.20.6859-6869.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Differential Interactions of Specific Nuclear Factor I Isoforms with the Glucocorticoid Receptor and STAT5 in the Cooperative Regulation of WAP Gene Transcription

Sudit S. Mukhopadhyay,1 Shannon L. Wyszomierski,1,dagger Richard M. Gronostajski,2 and Jeffrey M. Rosen1,*

Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030,1 and Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 441952

Received 20 April 2001/Returned for modification 26 June 2001/Accepted 11 July 2001

The distal region (-830 to -720 bp) of the rat whey acidic protein (WAP) gene contains a composite response element (CoRE), which has been demonstrated previously to confer mammary gland-specific and hormonally regulated WAP gene expression. Point mutations in the binding sites for specific transcription factors present within this CoRE have demonstrated the importance of both nuclear factor I (NFI) and STAT5 as well as cooperative interactions with the glucocorticoid receptor (GR) in the regulation of WAP gene expression in the mammary gland of transgenic mice. This study reports the characterization of NFI gene expression during mammary gland development and the identification and cloning of specific NFI isoforms (NFI-A4, NFI-B2, and NFI-X1) from the mouse mammary gland during lactation. Some but not all of these NFI isoforms synergistically activate WAP gene transcription in cooperation with GR and STAT5, as determined using transient cotransfection assays in JEG-3 cells. On both the WAP CoRE and the mouse mammary tumor virus long terminal repeat promoter, the NFI-B isoform preferentially activated gene transcription in cooperation with STAT5A and GR. In contrast, the NFI-A isoform suppressed GR and STAT cooperativity at the WAP CoRE. Finally, unlike their interaction with the NFI consensus binding site in the adenovirus promoter, the DNA-binding specificities of the three NFI isoforms to the palindromic NFI site in the WAP CoRE were not identical, which may partially explain the failure of the NFI-A isoform to cooperate with GR and STAT5A.

* Corresponding author. Mailing address: Dept. of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030. Phone: (713) 798-6210. Fax: (713) 798-8012. E-mail: jrosen{at}bcm.tmc.edu.

dagger Present address: Department of Biochemistry, M. D. Anderson Cancer Center, Houston, TX 77030.

Molecular and Cellular Biology, October 2001, p. 6859-6869, Vol. 21, No. 20
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.20.6859-6869.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lee, T.-Y., Lee, D.-S., Kim, H.-M., Ko, J. S., Gronostajski, R. M., Cho, M.-I., Son, H.-H., Park, J.-C. (2009). Disruption of Nfic Causes Dissociation of Odontoblasts by Interfering With the Formation of Intercellular Junctions and Aberrant Odontoblast Differentiation. J. Histochem. Cytochem. 57: 469-476 [Abstract] [Full Text]  
  • Xiao, Z., Zhang, S., Magenheimer, B. S., Luo, J., Quarles, L. D. (2008). Polycystin-1 Regulates Skeletogenesis through Stimulation of the Osteoblast-specific Transcription Factor RUNX2-II. J. Biol. Chem. 283: 12624-12634 [Abstract] [Full Text]  
  • Yang, J.-Q., Rudiger, J. J., Hughes, J. M., Goulet, S., Gencay-Cornelson, M. M., Borger, P., Tamm, M., Roth, M. (2008). Cell Density and Serum Exposure Modify the Function of the Glucocorticoid Receptor C/EBP Complex. Am. J. Respir. Cell Mol. Bio. 38: 414-422 [Abstract] [Full Text]  
  • Newton, R., Holden, N. S. (2007). Separating Transrepression and Transactivation: A Distressing Divorce for the Glucocorticoid Receptor?. Mol. Pharmacol. 72: 799-809 [Abstract] [Full Text]  
  • Xu, R., Spencer, V. A., Bissell, M. J. (2007). Extracellular Matrix-regulated Gene Expression Requires Cooperation of SWI/SNF and Transcription Factors. J. Biol. Chem. 282: 14992-14999 [Abstract] [Full Text]  
  • Ouellet, S., Vigneault, F., Lessard, M., Leclerc, S., Drouin, R., Guerin, S. L. (2006). Transcriptional regulation of the cyclin-dependent kinase inhibitor 1A (p21) gene by NFI in proliferating human cells. Nucleic Acids Res 34: 6472-6487 [Abstract] [Full Text]  
  • Gardmo, C., Mode, A. (2006). In vivo transfection of rat liver discloses binding sites conveying GH-dependent and female-specific gene expression. J Mol Endocrinol 37: 433-441 [Abstract] [Full Text]  
  • Vanselow, J., Yang, W., Herrmann, J., Zerbe, H., Schuberth, H.-J., Petzl, W., Tomek, W., Seyfert, H.-M. (2006). DNA-remethylation around a STAT5-binding enhancer in the {alpha}S1-casein promoter is associated with abrupt shutdown of {alpha}S1-casein synthesis during acute mastitis. J Mol Endocrinol 37: 463-477 [Abstract] [Full Text]  
  • Mukhopadhyay, S. S., Leung, K. S., Hicks, M. J., Hastings, P. J., Youssoufian, H., Plon, S. E. (2006). Defective mitochondrial peroxiredoxin-3 results in sensitivity to oxidative stress in Fanconi anemia. JCB 175: 225-235 [Abstract] [Full Text]  
  • Gopalan, S. M., Wilczynska, K. M., Konik, B. S., Bryan, L., Kordula, T. (2006). Nuclear Factor-1-X Regulates Astrocyte-specific Expression of the {alpha}1-Antichymotrypsin and Glial Fibrillary Acidic Protein Genes. J. Biol. Chem. 281: 13126-13133 [Abstract] [Full Text]  
  • Holloway, M. G., Laz, E. V., Waxman, D. J. (2006). Codependence of Growth Hormone-Responsive, Sexually Dimorphic Hepatic Gene Expression on Signal Transducer and Activator of Transcription 5b and Hepatic Nuclear Factor 4{alpha}. Mol. Endocrinol. 20: 647-660 [Abstract] [Full Text]  
  • Clarkson, R. W. E., Boland, M. P., Kritikou, E. A., Lee, J. M., Freeman, T. C., Tiffen, P. G., Watson, C. J. (2006). The Genes Induced by Signal Transducer and Activators of Transcription (STAT)3 and STAT5 in Mammary Epithelial Cells Define the Roles of these STATs in Mammary Development. Mol. Endocrinol. 20: 675-685 [Abstract] [Full Text]  
  • Shimizu, M., Akter, Mst. H., Emi, Y., Sato, R., Yamaguchi, T., Hirose, F., Osumi, T. (2006). Peroxisome Proliferator-Activated Receptor Subtypes Differentially Cooperate with Other Transcription Factors in Selective Transactivation of the Perilipin/PEX11{alpha} Gene Pair.. J Biochem 139: 563-573 [Abstract] [Full Text]  
  • Johansson, E. M., Kannius-Janson, M., Gritli-Linde, A., Bjursell, G., Nilsson, J. (2005). Nuclear Factor 1-C2 Is Regulated by Prolactin and Shows a Distinct Expression Pattern in the Mouse Mammary Epithelial Cells during Development. Mol. Endocrinol. 19: 992-1003 [Abstract] [Full Text]  
  • Wickenheisser, J. K., Nelson-DeGrave, V. L., Quinn, P. G., McAllister, J. M. (2004). Increased Cytochrome P450 17{alpha}-Hydroxylase Promoter Function in Theca Cells Isolated from Patients with Polycystic Ovary Syndrome Involves Nuclear Factor-1. Mol. Endocrinol. 18: 588-605 [Abstract] [Full Text]  
  • Bromberg-White, J. L., Sen, E., Alam, S., Bodily, J. M., Meyers, C. (2003). Induction of the Upstream Regulatory Region of Human Papillomavirus Type 31 by Dexamethasone Is Differentiation Dependent. J. Virol. 77: 10975-10983 [Abstract] [Full Text]  
  • Zambotti, A., Makhluf, H., Shen, J., Ducy, P. (2002). Characterization of an Osteoblast-specific Enhancer Element in the CBFA1 Gene. J. Biol. Chem. 277: 41497-41506 [Abstract] [Full Text]  
  • Kingsley-Kallesen, M., Mukhopadhyay, S. S., Wyszomierski, S. L., Schanler, S., Schutz, G., Rosen, J. M. (2002). The Mineralocorticoid Receptor May Compensate for the Loss of the Glucocorticoid Receptor at Specific Stages of Mammary Gland Development. Mol. Endocrinol. 16: 2008-2018 [Abstract] [Full Text]  
  • Kannius-Janson, M., Johansson, E. M., Bjursell, G., Nilsson, J. (2002). Nuclear Factor 1-C2 Contributes to the Tissue-specific Activation of a Milk Protein Gene in the Differentiating Mammary Gland. J. Biol. Chem. 277: 17589-17596 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»