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Molecular and Cellular Biology, November 2001, p. 7449-7459, Vol. 21, No. 21
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.21.7449-7459.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inhibition of Protein Kinase B (PKB) and PKCzeta Mediates Keratin K10-Induced Cell Cycle Arrest

Jesus M. Paramio, Carmen Segrelles, Sergio Ruiz, and José L. Jorcano*

Project on Cell and Molecular Biology and Gene Therapy, CIEMAT, E-28040 Madrid, Spain

Received 21 February 2001/Returned for modification 17 April 2001/Accepted 18 July 2001

The intermediate filament cytoskeleton is composed of keratins in all epithelial cells and imparts mechanical integrity to these cells. However, beyond this shared function, the functional significance of the carefully regulated tissue- and differentiation-specific expression of the large keratin family of cytoskeletal proteins remains unclear. We recently demonstrated that expression of keratin K10 or K16 may regulate the phosphorylation of the retinoblastoma protein (pRb), inhibiting (K10) or stimulating (K16) cell proliferation (J. M. Paramio, M. L. Casanova, C. Segrelles, S. Mittnacht, E. B. Lane, and J. L. Jorcano, Mol. Cell. Biol. 19:3086-3094, 1999). Here we show that keratin K10 function as a negative modulator of cell cycle progression involves changes in the phosphoinositide 3-kinase (PI-3K) signal transduction pathway. Physical interaction of K10 with Akt (protein kinase B [PKB]) and atypical PKCzeta causes sequestration of these kinases within the cytoskeleton and inhibits their intracellular translocation. As a consequence, the expression of K10 impairs the activation of PKB and PKCzeta . We also demonstrate that this inhibition impedes pRb phosphorylation and reduces the expression of cyclins D1 and E. Functional and biochemical data also demonstrate that the interaction between K10 and these kinases involves the non-alpha -helical amino domain of K10 (NTerm). Together, these results suggest new and essential roles for the keratins as modulators of specific signal transduction pathways.

* Corresponding author. Mailing address: Project on Cell and Molecular Biology, CIEMAT, Av. Complutense 22, E-28040 Madrid, Spain. Phone: 34 91 3466598. Fax: 34 91 3466393. E-mail: jl.jorcano{at}ciemat.es.

Molecular and Cellular Biology, November 2001, p. 7449-7459, Vol. 21, No. 21
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.21.7449-7459.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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