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Molecular and Cellular Biology, November 2001, p. 7523-7534, Vol. 21, No. 21
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.21.7523-7534.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

TAFII170 Interacts with the Concave Surface of TATA-Binding Protein To Inhibit Its DNA Binding Activity

Lloyd A. Pereira, Jan A. van der Knaap,dagger Vincent van den Boom,Dagger Fiona A. J. van den Heuvel,§ and H. T. Marc Timmers*

Department of Physiological Chemistry, University Medical Center Utrecht, 3508 AB Utrecht, The Netherlands

Received 11 June 2001/Returned for modification 9 July 2001/Accepted 6 August 2001

The human RNA polymerase II transcription factor B-TFIID consists of TATA-binding protein (TBP) and the TBP-associated factor (TAF) TAFII170 and can rapidly redistribute over promoter DNA. Here we report the identification of human TBP-binding regions in human TAFII170. We have defined the TBP interaction domain of TAFII170 within three amino-terminal regions: residues 2 to 137, 290 to 381, and 380 to 460. Each region contains a pair of Huntington-elongation-A subunit-Tor repeats and exhibits species-specific interactions with TBP family members. Remarkably, the altered-specificity TBP mutant (TBPAS) containing a triple mutation in the concave surface is defective for binding the TAFII170 amino-terminal region of residues 1 to 504. Furthermore, within this region the TAFII170 residues 290 to 381 can inhibit the interaction between Drosophila TAFII230 (residues 2 to 81) and TBP through competition for the concave surface of TBP. Biochemical analyses of TBP binding to the TATA box indicated that TAFII170 region 290-381 inhibits TBP-DNA complex formation. Importantly, the TBPAS mutant is less sensitive to TAFII170 inhibition. Collectively, our results support a mechanism in which TAFII170 induces high-mobility DNA binding by TBP through reversible interactions with its concave DNA binding surface.

* Corresponding author. Mailing address: Department of Physiological Chemistry, University Medical Center Utrecht, P.O. Box 85060, 3508 AB Utrecht, The Netherlands. Phone: 31-30-253-8981. Fax: 31-30-253-9035. E-mail: h.t.m.timmers{at}med.uu.nl.

dagger Present address: Faculty of Medicine, Department of Molecular Cell Biology, Leiden University, 2300 RA Leiden, The Netherlands.

Dagger Present address: Medical Genetics Centre, Erasmus University Rotterdam, 3000 DR Rotterdam, The Netherlands.

§ Present address: Department of Gynecology, University Hospital Groningen, 9700 RB Groningen, The Netherlands.

Molecular and Cellular Biology, November 2001, p. 7523-7534, Vol. 21, No. 21
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.21.7523-7534.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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