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Molecular and Cellular Biology, November 2001, p. 7696-7706, Vol. 21, No. 22
Department of Biochemistry and Molecular
Biology, Mount Sinai School of Medicine, New York, New York 10029
Received 14 March 2001/Returned for modification 17 April
2001/Accepted 8 August 2001
Determination and differentiation of skeletal muscle precursors
requires cell-cell contact, but the full range of cell surface proteins
that mediate this requirement and the mechanisms by which they work are
not known. To identify participants in cell contact-mediated regulation
of myogenesis, genes that encode secreted proteins specifically
upregulated during differentiation of C2C12 myoblasts were identified
by the yeast signal sequence trap method (K. A. Jacobs, L. A. Collins-Racie, M. Colbert, M. Duckett, M. Golden-Fleet, K. Kelleher, R. Kriz, E. R. La Vallie, D. Merberg, V. Spaulding, J. Stover,
M. J. Williamson, and J. M. McCoy, Gene
198:289-296, 1997), followed by RNA expression analysis.
We report here the identification of CD164 as a gene expressed in
proliferating C2C12 cells that is upregulated during differentiation.
CD164 encodes a widely expressed cell surface sialomucin that has been
implicated in regulation of cell proliferation and adhesion during
hematopoiesis. Stable overexpression of CD164 in C2C12 and F3 myoblasts
enhanced their differentiation, as assessed by both morphological and
biochemical criteria. Furthermore, expression of antisense CD164 or
soluble extracellular regions of CD164 inhibited myogenic
differentiation. Treatment of C2C12 cells with sialidase or
O-sialoglycoprotease, two enzymes previously reported to
destroy functional epitopes on CD164, also inhibited differentiation.
These data indicate that (i) CD164 may play a rate-limiting role in
differentiation of cultured myoblasts, (ii) sialomucins represent a
class of potential effectors of cell contact-mediated regulation of
myogenesis, and (iii) carbohydrate-based cell recognition may play a
role in mediating this phenomenon.
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.22.7696-7706.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Identification of a Role for the Sialomucin CD164
in Myogenic Differentiation by Signal Sequence Trapping in
Yeast

*
Corresponding author. Mailing address: Department of
Biochemistry and Molecular Biology, Box 1020, Mount Sinai School of
Medicine, New York, NY 10029. Phone: (212) 241-2177. Fax: (212)
996-7214. E-mail: Robert.Krauss{at}mssm.edu.
Present address: Discovery Group, SK Corporation, Yusung-gu,
Taejon, 305-712, Korea.
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