p300 Acts as a Transcriptional Coactivator for Mammalian Notch-1

doi:10.1128/MCB.21.22.7761-7774.2001

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Oswald, F.
	Articles by Schmid, R. M.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Oswald, F.
	Articles by Schmid, R. M.

Previous Article | Next Article

Molecular and Cellular Biology, November 2001, p. 7761-7774, Vol. 21, No. 22
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.22.7761-7774.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

p300 Acts as a Transcriptional Coactivator for Mammalian Notch-1

Franz Oswald,¹ Birgitt Täuber,² Thomas Dobner,² Soizic Bourteele,¹ Ulrike Kostezka,¹ Guido Adler,¹ Susanne Liptay,³ and Roland M. Schmid¹^,^*

Departments of Internal Medicine¹ and Pediatrics,³ University of Ulm, D-89081 Ulm, and Institute for Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg,² Germany

Received 16 March 2001/Returned for modification 29 April 2001/Accepted 2 August 2001

Notch-1 belongs to a family of transmembrane receptor proteins that direct the decisions as to various cell fates. After ligandbinding, a proteolytic cleavage step occurs and the intracellularpart of Notch-1, Notch-1-IC, translocates into the nucleus, whereit targets the DNA binding protein RBP-J $kappa$ /CBF1. RBP-J $kappa$ mediatesrepression through recruitment of a histone deacetylase-containingcomplex. The Notch-1-IC/RBP-J $kappa$ complex overcomes repression andactivates the transcription of Notch target genes. We have identifieda novel domain in Notch-1-IC, the EP domain, which is indispensablefor full transcriptional activation. This transactivation domainis localized adjacent to the ankyrin repeats of Notch-1-IC. Incotransfection experiments, Notch-1-IC-mediated transcriptionalactivation was inhibited by E1A12S and p53, two proteins, whichinterfere with the function of the common coactivator p300. Protein-proteininteraction assays demonstrated the association of Notch-1-ICand the CH3 region of p300. In addition, the interaction of mammalianNotch-1-IC with p300 was destabilized after deletion of the EPdomain of Notch-1-IC. Based on physical interaction with Notch-1-ICand coactivator functions of p300, we propose a model for Notch-1-mediatedgene regulation viap300.

^* Corresponding author. Mailing address: Department of Internal Medicine I, Robert-Koch-Strasse 8, D-89081 Ulm, Germany. Phone:49-731-50-24305. Fax: 49-731-50-24302. E-mail: roland.schmid{at}medizin.uni-ulm.de.

This article has been cited by other articles:

Samon, J. B., Champhekar, A., Minter, L. M., Telfer, J. C., Miele, L., Fauq, A., Das, P., Golde, T. E., Osborne, B. A. (2008). Notch1 and TGF{beta}1 cooperatively regulate Foxp3 expression and the maintenance of peripheral regulatory T cells. Blood 112: 1813-1821 [Abstract] [Full Text]
Salat, D., Liefke, R., Wiedenmann, J., Borggrefe, T., Oswald, F. (2008). ETO, but Not Leukemogenic Fusion Protein AML1/ETO, Augments RBP-J{kappa}/SHARP-Mediated Repression of Notch Target Genes. Mol. Cell. Biol. 28: 3502-3512 [Abstract] [Full Text]
Elyaman, W., Bradshaw, E. M., Wang, Y., Oukka, M., Kivisakk, P., Chiba, S., Yagita, H., Khoury, S. J. (2007). Jagged1 and Delta1 Differentially Regulate the Outcome of Experimental Autoimmune Encephalomyelitis. J. Immunol. 179: 5990-5998 [Abstract] [Full Text]
Reineke, E. L., Liu, H., Lam, M., Liu, Y., Kao, H.-Y. (2007). Aberrant Association of Promyelocytic Leukemia Protein-Retinoic Acid Receptor-{alpha} with Coactivators Contributes to Its Ability to Regulate Gene Expression. J. Biol. Chem. 282: 18584-18596 [Abstract] [Full Text]
Krejci, A., Bray, S. (2007). Notch activation stimulates transient and selective binding of Su(H)/CSL to target enhancers. Genes Dev. 21: 1322-1327 [Abstract] [Full Text]
Lefort, K., Mandinova, A., Ostano, P., Kolev, V., Calpini, V., Kolfschoten, I., Devgan, V., Lieb, J., Raffoul, W., Hohl, D., Neel, V., Garlick, J., Chiorino, G., Dotto, G. P. (2007). Notch1 is a p53 target gene involved in human keratinocyte tumor suppression through negative regulation of ROCK1/2 and MRCK{alpha} kinases. Genes Dev. 21: 562-577 [Abstract] [Full Text]
Sharma, V. M., Calvo, J. A., Draheim, K. M., Cunningham, L. A., Hermance, N., Beverly, L., Krishnamoorthy, V., Bhasin, M., Capobianco, A. J., Kelliher, M. A. (2006). Notch1 Contributes to Mouse T-Cell Leukemia by Directly Inducing the Expression of c-myc. Mol. Cell. Biol. 26: 8022-8031 [Abstract] [Full Text]
Doi, H., Iso, T., Sato, H., Yamazaki, M., Matsui, H., Tanaka, T., Manabe, I., Arai, M., Nagai, R., Kurabayashi, M. (2006). Jagged1-selective Notch Signaling Induces Smooth Muscle Differentiation via a RBP-J{kappa}-dependent Pathway. J. Biol. Chem. 281: 28555-28564 [Abstract] [Full Text]
Stallwood, Y., Briend, E., Ray, K. M., Ward, G. A., Smith, B. J., Nye, E., Champion, B. R., McKenzie, G. J. (2006). Small Interfering RNA-Mediated Knockdown of Notch Ligands in Primary CD4+ T Cells and Dendritic Cells Enhances Cytokine Production. J. Immunol. 177: 885-895 [Abstract] [Full Text]
Mungamuri, S. K., Yang, X., Thor, A. D., Somasundaram, K. (2006). Survival Signaling by Notch1: Mammalian Target of Rapamycin (mTOR)-Dependent Inhibition of p53.. Cancer Res. 66: 4715-4724 [Abstract] [Full Text]
Nguyen, B.-C., Lefort, K., Mandinova, A., Antonini, D., Devgan, V., Della Gatta, G., Koster, M. I., Zhang, Z., Wang, J., di Vignano, A. T., Kitajewski, J., Chiorino, G., Roop, D. R., Missero, C., Dotto, G. P. (2006). Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation. Genes Dev. 20: 1028-1042 [Abstract] [Full Text]
Gause, M., Eissenberg, J. C., MacRae, A. F., Dorsett, M., Misulovin, Z., Dorsett, D. (2006). Nipped-A, the Tra1/TRRAP Subunit of the Drosophila SAGA and Tip60 Complexes, Has Multiple Roles in Notch Signaling during Wing Development.. Mol. Cell. Biol. 26: 2347-2359 [Abstract] [Full Text]
Zhao, B., Maruo, S., Cooper, A., R. Chase, M., Johannsen, E., Kieff, E., Cahir-McFarland, E. (2006). RNAs induced by Epstein-Barr virus nuclear antigen 2 in lymphoblastoid cell lines. Proc. Natl. Acad. Sci. USA 103: 1900-1905 [Abstract] [Full Text]
Kasper, L. H., Fukuyama, T., Biesen, M. A., Boussouar, F., Tong, C., de Pauw, A., Murray, P. J., van Deursen, J. M. A., Brindle, P. K. (2006). Conditional Knockout Mice Reveal Distinct Functions for the Global Transcriptional Coactivators CBP and p300 in T-Cell Development. Mol. Cell. Biol. 26: 789-809 [Abstract] [Full Text]
Oswald, F., Winkler, M., Cao, Y., Astrahantseff, K., Bourteele, S., Knochel, W., Borggrefe, T. (2005). RBP-J{kappa}/SHARP Recruits CtIP/CtBP Corepressors To Silence Notch Target Genes. Mol. Cell. Biol. 25: 10379-10390 [Abstract] [Full Text]
Nagel, A. C., Krejci, A., Tenin, G., Bravo-Patino, A., Bray, S., Maier, D., Preiss, A. (2005). Hairless-Mediated Repression of Notch Target Genes Requires the Combined Activity of Groucho and CtBP Corepressors. Mol. Cell. Biol. 25: 10433-10441 [Abstract] [Full Text]
Lubman, O. Y., Kopan, R., Waksman, G., Korolev, S. (2005). The crystal structure of a partial mouse Notch-1 ankyrin domain: Repeats 4 through 7 preserve an ankyrin fold. Protein Sci. 14: 1274-1281 [Abstract] [Full Text]
Lan, K., Kuppers, D. A., Robertson, E. S. (2005). Kaposi's Sarcoma-Associated Herpesvirus Reactivation Is Regulated by Interaction of Latency-Associated Nuclear Antigen with Recombination Signal Sequence-Binding Protein J{kappa}, the Major Downstream Effector of the Notch Signaling Pathway. J. Virol. 79: 3468-3478 [Abstract] [Full Text]
Yeh, T.-S., Hsieh, R.-H., Shen, S.-C., Wang, S.-H., Tseng, M.-J., Shih, C.-M., Lin, J.-J. (2004). Nuclear {beta}II-Tubulin Associates with the Activated Notch Receptor to Modulate Notch Signaling. Cancer Res. 64: 8334-8340 [Abstract] [Full Text]
Wiedenmann, J., Ivanchenko, S., Oswald, F., Schmitt, F., Rocker, C., Salih, A., Spindler, K.-D., Nienhaus, G. U. (2004). EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc. Natl. Acad. Sci. USA 101: 15905-15910 [Abstract] [Full Text]
MacKenzie, F., Duriez, P., Larrivee, B., Chang, L., Pollet, I., Wong, F., Yip, C., Karsan, A. (2004). Notch4-induced inhibition of endothelial sprouting requires the ankyrin repeats and involves signaling through RBP-J{kappa}. Blood 104: 1760-1768 [Abstract] [Full Text]
Le Gall, M., Giniger, E. (2004). Identification of Two Binding Regions for the Suppressor of Hairless Protein within the Intracellular Domain of Drosophila Notch. J. Biol. Chem. 279: 29418-29426 [Abstract] [Full Text]
Chu, J., Bresnick, E. H. (2004). Evidence That C Promoter-binding Factor 1 Binding Is Required for Notch-1-mediated Repression of Activator Protein-1. J. Biol. Chem. 279: 12337-12345 [Abstract] [Full Text]
Blokzijl, A., Dahlqvist, C., Reissmann, E., Falk, A., Moliner, A., Lendahl, U., Ibanez, C. F. (2003). Cross-talk between the Notch and TGF-{beta} signaling pathways mediated by interaction of the Notch intracellular domain with Smad3. J. Cell Biol. 163: 723-728 [Abstract] [Full Text]
Yeh, T.-S., Lin, Y.-M., Hsieh, R.-H., Tseng, M.-J. (2003). Association of Transcription Factor YY1 with the High Molecular Weight Notch Complex Suppresses the Transactivation Activity of Notch. J. Biol. Chem. 278: 41963-41969 [Abstract] [Full Text]
Takizawa, T., Ochiai, W., Nakashima, K., Taga, T. (2003). Enhanced gene activation by Notch and BMP signaling cross-talk. Nucleic Acids Res 31: 5723-5731 [Abstract] [Full Text]
Espinosa, L., Ingles-Esteve, J., Aguilera, C., Bigas, A. (2003). Phosphorylation by Glycogen Synthase Kinase-3{beta} Down-regulates Notch Activity, a Link for Notch and Wnt Pathways. J. Biol. Chem. 278: 32227-32235 [Abstract] [Full Text]
Oakley, F., Mann, J., Ruddell, R. G., Pickford, J., Weinmaster, G., Mann, D. A. (2003). Basal Expression of I{kappa}B{alpha} Is Controlled by the Mammalian Transcriptional Repressor RBP-J (CBF1) and Its Activator Notch1. J. Biol. Chem. 278: 24359-24370 [Abstract] [Full Text]
Zhao, F., McCarrick-Walmsley, R., Akerblad, P., Sigvardsson, M., Kadesch, T. (2003). Inhibition of p300/CBP by Early B-Cell Factor. Mol. Cell. Biol. 23: 3837-3846 [Abstract] [Full Text]
Nowling, T., Bernadt, C., Johnson, L., Desler, M., Rizzino, A. (2003). The Co-activator p300 Associates Physically with and Can Mediate the Action of the Distal Enhancer of the FGF-4 Gene. J. Biol. Chem. 278: 13696-13705 [Abstract] [Full Text]
Wallberg, A. E., Pedersen, K., Lendahl, U., Roeder, R. G. (2002). p300 and PCAF Act Cooperatively To Mediate Transcriptional Activation from Chromatin Templates by Notch Intracellular Domains In Vitro. Mol. Cell. Biol. 22: 7812-7819 [Abstract] [Full Text]
Wu, L., Sun, T., Kobayashi, K., Gao, P., Griffin, J. D. (2002). Identification of a Family of Mastermind-Like Transcriptional Coactivators for Mammalian Notch Receptors. Mol. Cell. Biol. 22: 7688-7700 [Abstract] [Full Text]
Suh, Y.-H., Checler, F. (2002). Amyloid Precursor Protein, Presenilins, and alpha -Synuclein: Molecular Pathogenesis and Pharmacological Applications in Alzheimer's Disease. Pharmacol. Rev. 54: 469-525 [Abstract] [Full Text]
Barolo, S., Stone, T., Bang, A. G., Posakony, J. W. (2002). Default repression and Notch signaling: Hairless acts as an adaptor to recruit the corepressors Groucho and dCtBP to Suppressor of Hairless. Genes Dev. 16: 1964-1976 [Abstract] [Full Text]
Liang, Y., Chang, J., Lynch, S. J., Lukac, D. M., Ganem, D. (2002). The lytic switch protein of KSHV activates gene expression via functional interaction with RBP-Jkappa (CSL), the target of the Notch signaling pathway. Genes Dev. 16: 1977-1989 [Abstract] [Full Text]
Jeffries, S., Robbins, D. J., Capobianco, A. J. (2002). Characterization of a High-Molecular-Weight Notch Complex in the Nucleus of Notchic-Transformed RKE Cells and in a Human T-Cell Leukemia Cell Line. Mol. Cell. Biol. 22: 3927-3941 [Abstract] [Full Text]
Fryer, C. J., Lamar, E., Turbachova, I., Kintner, C., Jones, K. A. (2002). Mastermind mediates chromatin-specific transcription and turnover of the Notch enhancer complex. Genes Dev. 16: 1397-1411 [Abstract] [Full Text]
Barolo, S., Posakony, J. W. (2002). Three habits of highly effective signaling pathways: principles of transcriptional control by developmental cell signaling. Genes Dev. 16: 1167-1181 [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals