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Molecular and Cellular Biology, November 2001, p. 7839-7851, Vol. 21, No. 22
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.22.7839-7851.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

A Truncation Mutant of the 95-Kilodalton Subunit of Transcription Factor IIIC Reveals Asymmetry in Ty3 Integration

Michael Aye,1 Sandra L. Dildine,1 Jonathan A. Claypool,1 Sabine Jourdain,2 and Suzanne B. Sandmeyer1,*

Department of Biological Chemistry, University of California, Irvine, California 92697,1 and Service de Biochimie et de Genetique Moleculaire, CEA/Saclay, F-91191 Gif-sur-Yvette Cedex, France2

Received 11 June 2001/Returned for modification 3 August 2001/Accepted 15 August 2001

Position-specific integration of the retroviruslike element Ty3 near the transcription initiation sites of tRNA genes requires transcription factors IIIB and IIIC (TFIIIB and TFIIIC). Using a genetic screen, we isolated a mutant with a truncated 95-kDa subunit of TFIIIC (TFIIIC95) that reduced the apparent retrotransposition of Ty3 into a plasmid-borne target site between two divergently transcribed tRNA genes. Although TFIIIC95 is conserved and essential, no defect in growth or transcription of tRNAs was detected in the mutant. Steps of the Ty3 life cycle, such as protein expression, proteolytic processing, viruslike particle formation, and reverse transcription, were not affected by the mutation. However, Ty3 integration into a divergent tDNA target occurred exclusively in one orientation in the mutant strain. Investigation of this orientation bias showed that TFIIIC95 and Ty3 integrase interacted in two-hybrid and glutathione S-transferase pulldown assays and that interaction with the mutant TFIIIC95 protein was attenuated. The orientation bias observed here suggests that even for wild-type Ty3, the protein complexes associated with the long terminal repeats are not equivalent in vivo.

* Corresponding author. Mailing address: Department of Biological Chemistry, University of California, Irvine, CA 92697. Phone: (949) 824-7571. Fax: (949) 824-2688. E-mail: sbsandme{at}uci.edu.

Molecular and Cellular Biology, November 2001, p. 7839-7851, Vol. 21, No. 22
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.22.7839-7851.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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