This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grossi, S.
Right arrow Articles by Shore, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grossi, S.
Right arrow Articles by Shore, D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2001, p. 8117-8128, Vol. 21, No. 23
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.23.8117-8128.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Telomere Formation by Rap1p Binding Site Arrays Reveals End-Specific Length Regulation Requirements and Active Telomeric Recombination

Simona Grossi, Alessandro Bianchi, Pascal Damay, and David Shore*

Department of Molecular Biology, University of Geneva, 1211 Geneva 4, Switzerland

Received 13 June 2001/Returned for modification 11 July 2001/Accepted 29 August 2001

Rap1p, the major telomere repeat binding protein in yeast, has been implicated in both de novo telomere formation and telomere length regulation. To characterize the role of Rap1p in these processes in more detail, we studied the generation of telomeres in vivo from linear DNA substrates containing defined arrays of Rap1p binding sites. Consistent with previous work, our results indicate that synthetic Rap1p binding sites within the internal half of a telomeric array are recognized as an integral part of the telomere complex in an orientation-independent manner that is largely insensitive to the precise spacing between adjacent sites. By extending the lengths of these constructs, we found that several different Rap1p site arrays could never be found at the very distal end of a telomere, even when correctly oriented. Instead, these synthetic arrays were always followed by a short (approx 100-bp) "cap" of genuine TG repeat sequence, indicating a remarkably strict sequence requirement for an end-specific function(s) of the telomere. Despite this fact, even misoriented Rap1p site arrays promote telomere formation when they are placed at the distal end of a telomere-healing substrate, provided that at least a single correctly oriented site is present within the array. Surprisingly, these heterogeneous arrays of Rap1p binding sites generate telomeres through a RAD52-dependent fusion resolution reaction that results in an inversion of the original array. Our results provide new insights into the nature of telomere end capping and reveal one way by which recombination can resolve a defect in this process.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland. Phone: 41-22-702-6183. Fax: 41-22-702-6868. E-mail: David.Shore{at}molbio.unige.ch.

Molecular and Cellular Biology, December 2001, p. 8117-8128, Vol. 21, No. 23
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.23.8117-8128.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bechard, L. H., Butuner, B. D., Peterson, G. J., McRae, W., Topcu, Z., McEachern, M. J. (2009). Mutant Telomeric Repeats in Yeast Can Disrupt the Negative Regulation of Recombination-Mediated Telomere Maintenance and Create an Alternative Lengthening of Telomeres-Like Phenotype. Mol. Cell. Biol. 29: 626-639 [Abstract] [Full Text]  
  • Negrini, S., Ribaud, V., Bianchi, A., Shore, D. (2007). DNA breaks are masked by multiple Rap1 binding in yeast: implications for telomere capping and telomerase regulation. Genes Dev. 21: 292-302 [Abstract] [Full Text]  
  • Levy, D. L., Blackburn, E. H. (2004). Counting of Rif1p and Rif2p on Saccharomyces cerevisiae Telomeres Regulates Telomere Length. Mol. Cell. Biol. 24: 10857-10867 [Abstract] [Full Text]  
  • Kim, S.-h., Beausejour, C., Davalos, A. R., Kaminker, P., Heo, S.-J., Campisi, J. (2004). TIN2 Mediates Functions of TRF2 at Human Telomeres. J. Biol. Chem. 279: 43799-43804 [Abstract] [Full Text]  
  • Grossi, S., Puglisi, A., Dmitriev, P. V., Lopes, M., Shore, D. (2004). Pol12, the B subunit of DNA polymerase {alpha}, functions in both telomere capping and length regulation. Genes Dev. 18: 992-1006 [Abstract] [Full Text]  
  • Underwood, D. H., Carroll, C., McEachern, M. J. (2004). Genetic Dissection of the Kluyveromyces lactis Telomere and Evidence for Telomere Capping Defects in TER1 Mutants with Long Telomeres. Eukaryot Cell 3: 369-384 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»