This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Calvo, S.
Right arrow Articles by Buonanno, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Calvo, S.
Right arrow Articles by Buonanno, A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, December 2001, p. 8490-8503, Vol. 21, No. 24
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.24.8490-8503.2001

Molecular Dissection of DNA Sequences and Factors Involved in Slow Muscle-Specific Transcription

Soledad Calvo,dagger Detlef Vullhorst, Pratap Venepally,Dagger Jun Cheng, Irina Karavanova, and Andres Buonanno*

Section on Molecular Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892

Received 1 May 2001/Returned for modification 25 June 2001/Accepted 18 September 2001

Transcription is a major regulatory mechanism for the generation of slow- and fast-twitch myofibers. We previously identified an upstream region of the slow TnI gene (slow upstream regulatory element [SURE]) and an intronic region of the fast TnI gene (fast intronic regulatory element [FIRE]) that are sufficient to direct fiber type-specific transcription in transgenic mice. Here we demonstrate that the downstream half of TnI SURE, containing E box, NFAT, MEF-2, and CACC motifs, is sufficient to confer pan-skeletal muscle-specific expression in transgenic mice. However, upstream regions of SURE and FIRE are required for slow and fast fiber type specificity, respectively. By adding back upstream SURE sequences to the pan-muscle-specific enhancer, we delineated a 15-bp region necessary for slow muscle specificity. Using this sequence in a yeast one-hybrid screen, we isolated cDNAs for general transcription factor 3 (GTF3)/muscle TFII-I repeat domain-containing protein 1 (MusTRD1). GTF3 is a multidomain nuclear protein related to initiator element-binding transcription factor TF II-I; the genes for both proteins are deleted in persons with Williams-Beuren syndrome, who often manifest muscle weakness. Gel retardation assays revealed that full-length GTF3, as well as its carboxy-terminal half, specifically bind the bicoid-like motif of SURE (GTTAATCCG). GTF3 expression is neither muscle nor fiber type specific. Its levels are highest during a period of fetal development that coincides with the emergence of specific fiber types and transiently increases in regenerating muscles damaged by bupivacaine. We further show that transcription from TnI SURE is repressed by GTF3 when overexpressed in electroporated adult soleus muscles. These results suggest a role for GTF3 as a regulator of slow TnI expression during early stages of muscle development and suggest how it could contribute to Williams-Beuren syndrome.

* Corresponding author. Mailing address: Section on Molecular Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892. Phone: (301) 496-3298. Fax: (301) 496-9939. E-mail: buonanno{at}helix.nih.gov.

dagger Present address: Universidad de Castilla la Mancha, Facultad de Medicina, Albacete 02071, Spain.

Dagger Present address: National Center for Biotechnology Information, NIH, Bethesda, MD 20894.

Molecular and Cellular Biology, December 2001, p. 8490-8503, Vol. 21, No. 24
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.24.8490-8503.2001


This article has been cited by other articles:

  • Tsika, R. W., Schramm, C., Simmer, G., Fitzsimons, D. P., Moss, R. L., Ji, J. (2008). Overexpression of TEAD-1 in Transgenic Mouse Striated Muscles Produces a Slower Skeletal Muscle Contractile Phenotype. J. Biol. Chem. 283: 36154-36167 [Abstract] [Full Text]  
  • Lazebnik, M. B., Tussie-Luna, M. I., Roy, A. L. (2008). Determination and Functional Analysis of the Consensus Binding Site for TFII-I Family Member BEN, Implicated in Williams-Beuren Syndrome. J. Biol. Chem. 283: 11078-11082 [Abstract] [Full Text]  
  • Rana, Z. A., Gundersen, K., Buonanno, A. (2008). Activity-dependent repression of muscle genes by NFAT. Proc. Natl. Acad. Sci. USA 105: 5921-5926 [Abstract] [Full Text]  
  • Vullhorst, D., Buonanno, A. (2005). Multiple GTF2I-like Repeats of General Transcription Factor 3 Exhibit DNA Binding Properties: EVIDENCE FOR A COMMON ORIGIN AS A SEQUENCE-SPECIFIC DNA INTERACTION MODULE. J. Biol. Chem. 280: 31722-31731 [Abstract] [Full Text]  
  • Allen, D. L., Weber, J. N., Sycuro, L. K., Leinwand, L. A. (2005). Myocyte Enhancer Factor-2 and Serum Response Factor Binding Elements Regulate Fast Myosin Heavy Chain Transcription in Vivo. J. Biol. Chem. 280: 17126-17134 [Abstract] [Full Text]  
  • Rana, Z. A, Gundersen, K., Buonanno, A., Vullhorst, D. (2005). Imaging transcription in vivo: distinct regulatory effects of fast and slow activity patterns on promoter elements from vertebrate troponin I isoform genes. J. Physiol. 562: 815-828 [Abstract] [Full Text]  
  • Cheng, G., Merriam, A. P., Gong, B., Leahy, P., Khanna, S., Porter, J. D. (2004). Conserved and muscle-group-specific gene expression patterns shape postnatal development of the novel extraocular muscle phenotype. Physiol. Genomics 18: 184-195 [Abstract] [Full Text]  
  • Lee, H. H. C., Choi, R. C. Y., Ting, A. K. L., Siow, N. L., Jiang, J. X. S., Massoulie, J., Tsim, K. W. K. (2004). Transcriptional Regulation of Acetylcholinesterase-associated Collagen ColQ: DIFFERENTIAL EXPRESSION IN FAST AND SLOW TWITCH MUSCLE FIBERS IS DRIVEN BY DISTINCT PROMOTERS. J. Biol. Chem. 279: 27098-27107 [Abstract] [Full Text]  
  • Mas, J.-A., Garcia-Zaragoza, E., Cervera, M. (2004). Two Functionally Identical Modular Enhancers in Drosophila Troponin T Gene Establish the Correct Protein Levels in Different Muscle Types. Mol. Biol. Cell 15: 1931-1945 [Abstract] [Full Text]  
  • Yang, P., Kupershmidt, S., Roden, D. M. (2004). Cloning and initial characterization of the human cardiac sodium channel (SCN5A) promoter. Cardiovasc Res 61: 56-65 [Abstract] [Full Text]  
  • Nguyen, Q.-G. V., Buskin, J. N., Himeda, C. L., Shield, M. A., Hauschka, S. D. (2003). Differences in the Function of Three Conserved E-boxes of the Muscle Creatine Kinase Gene in Cultured Myocytes and in Transgenic Mouse Skeletal and Cardiac Muscle. J. Biol. Chem. 278: 46494-46505 [Abstract] [Full Text]  
  • Tassabehji, M. (2003). Williams-Beuren syndrome: a challenge for genotype-phenotype correlations. Hum Mol Genet 12: R229-237 [Abstract] [Full Text]  
  • Leary, S. C., Lyons, C. N., Rosenberger, A. G., Ballantyne, J. S., Stillman, J., Moyes, C. D. (2003). Fiber-type differences in muscle mitochondrial profiles. Am. J. Physiol. Regul. Integr. Comp. Physiol. 285: R817-R826 [Abstract] [Full Text]  
  • Polly, P., Haddadi, L. M., Issa, L. L., Subramaniam, N., Palmer, S. J., Tay, E. S. E., Hardeman, E. C. (2003). hMusTRD1{alpha}1 Represses MEF2 Activation of the Troponin I Slow Enhancer. J. Biol. Chem. 278: 36603-36610 [Abstract] [Full Text]  
  • Karasseva, N., Tsika, G., Ji, J., Zhang, A., Mao, X., Tsika, R. (2003). Transcription Enhancer Factor 1 Binds Multiple Muscle MEF2 and A/T-Rich Elements during Fast-to-Slow Skeletal Muscle Fiber Type Transitions. Mol. Cell. Biol. 23: 5143-5164 [Abstract] [Full Text]  
  • Vullhorst, D., Buonanno, A. (2003). Characterization of General Transcription Factor 3, a Transcription Factor Involved in Slow Muscle-specific Gene Expression. J. Biol. Chem. 278: 8370-8379 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»