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Molecular and Cellular Biology, February 2001, p. 765-770, Vol. 21, No. 3
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.3.765-770.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Cloning and Functional Analysis of Mouse C-Terminal Kinesin Motor KifC3

Zhaohuai Yang,1 Chun-hong Xia,1 Elizabeth A. Roberts,1 Kevin Bush,2 Sanjay K. Nigam,2 and Lawrence S. B. Goldstein1,*

Howard Hughes Medical Institute, Department of Cellular and Molecular Medicine,1 and Howard Hughes Medical Institute, Departments of Pediatrics and Medicine,2 University of California San Diego, La Jolla, California 92093

Received 7 November 2000/Accepted 10 November 2000

Proteins of the kinesin superfamily define a class of microtubule-dependent motors that play crucial roles in cell division and intracellular transport. To study the molecular mechanism of intracellular transport involving microtubule-dependent motors, a cDNA encoding a new kinesin-like protein called KifC3 was cloned from a mouse brain cDNA library. Sequence and secondary structure analysis revealed that KifC3 is a member of the C-terminal motor family. In contrast to other mouse C-terminal motors, KifC3 is apparently ubiquitous and may have a general role in intracellular transport. To understand the in vivo function of the KifC3 gene, we used homologous recombination in embryonic stem cells to construct knockout mouse strains for the KifC3 gene. Homozygous mutants of the KifC3 gene are viable, reproduce normally, and apparently develop normally. These results suggest that KifC3 is dispensable for normal development and reproduction in the mouse.

* Corresponding author. Mailing address: HHMI/CMM, Room 334, University of California San Diego, 9500 Gilman Dr., La Jolla, CA 92093-0683. Phone: (858) 534-9702. Fax: (858) 534-9701. E-mail: lgoldstein{at}ucsd.edu.

Molecular and Cellular Biology, February 2001, p. 765-770, Vol. 21, No. 3
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.3.765-770.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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