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Molecular and Cellular Biology, March 2001, p. 1573-1580, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1573-1580.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

VAMP3 Null Mice Display Normal Constitutive, Insulin- and Exercise-Regulated Vesicle Trafficking

Chunmei Yang,1 Silvia Mora,1 Jeffrey W. Ryder,1 Kenneth J. Coker,1,dagger Polly Hansen,2 Lee-Ann Allen,3 and Jeffrey E. Pessin1,*

Department of Physiology and Biophysics1 and Department of Internal Medicine,3 The University of Iowa, Iowa City, Iowa 52242, and Department of Cell Biology, Pfizer Global Research and Development, Ann Arbor, Michigan 481052

Received 19 September 2000/Returned for modification 28 September 2000/Accepted 27 November 2000

To investigate the physiological function of the VAMP3 vesicle SNARE (v-SNARE) isoform in the regulation of GLUT4 vesicle trafficking, we generated homozygotic VAMP3 null mice by targeted gene disruption. The VAMP3 null mice had typical growth rate and weight gain, with normal maintenance of fasting serum glucose and insulin levels. Analysis of glucose disposal and insulin sensitivity demonstrated normal insulin and glucose tolerance, with no evidence for insulin resistance. Insulin stimulation of glucose uptake in isolated primary adipocytes was essentially the same for the wild-type and VAMP3 null mice. Similarly, insulin-, hypoxia-, and exercise-stimulated glucose uptake in isolated skeletal muscle did not differ significantly. In addition, other general membrane trafficking events including phagocytosis, pinocytosis, and transferrin receptor recycling were also found to be unaffected in the VAMP3 null mice. Taken together, these data demonstrate that VAMP3 function is not necessary for either regulated GLUT4 translocation or general constitutive membrane recycling.

* Corresponding author. Mailing address: Department of Physiology and Biophysics, The University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7823. Fax: (319) 335-7886. E-mail: Jeffrey-Pessin{at}uiowa.edu.

dagger Present address: Lexicon Genetics Incorporated, The Woodlands, TX 77381.

Molecular and Cellular Biology, March 2001, p. 1573-1580, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1573-1580.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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