This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, C.
Right arrow Articles by Pessin, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, C.
Right arrow Articles by Pessin, J. E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2001, p. 1573-1580, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1573-1580.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

VAMP3 Null Mice Display Normal Constitutive, Insulin- and Exercise-Regulated Vesicle Trafficking

Chunmei Yang,1 Silvia Mora,1 Jeffrey W. Ryder,1 Kenneth J. Coker,1,dagger Polly Hansen,2 Lee-Ann Allen,3 and Jeffrey E. Pessin1,*

Department of Physiology and Biophysics1 and Department of Internal Medicine,3 The University of Iowa, Iowa City, Iowa 52242, and Department of Cell Biology, Pfizer Global Research and Development, Ann Arbor, Michigan 481052

Received 19 September 2000/Returned for modification 28 September 2000/Accepted 27 November 2000

To investigate the physiological function of the VAMP3 vesicle SNARE (v-SNARE) isoform in the regulation of GLUT4 vesicle trafficking, we generated homozygotic VAMP3 null mice by targeted gene disruption. The VAMP3 null mice had typical growth rate and weight gain, with normal maintenance of fasting serum glucose and insulin levels. Analysis of glucose disposal and insulin sensitivity demonstrated normal insulin and glucose tolerance, with no evidence for insulin resistance. Insulin stimulation of glucose uptake in isolated primary adipocytes was essentially the same for the wild-type and VAMP3 null mice. Similarly, insulin-, hypoxia-, and exercise-stimulated glucose uptake in isolated skeletal muscle did not differ significantly. In addition, other general membrane trafficking events including phagocytosis, pinocytosis, and transferrin receptor recycling were also found to be unaffected in the VAMP3 null mice. Taken together, these data demonstrate that VAMP3 function is not necessary for either regulated GLUT4 translocation or general constitutive membrane recycling.

* Corresponding author. Mailing address: Department of Physiology and Biophysics, The University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7823. Fax: (319) 335-7886. E-mail: Jeffrey-Pessin{at}uiowa.edu.

dagger Present address: Lexicon Genetics Incorporated, The Woodlands, TX 77381.

Molecular and Cellular Biology, March 2001, p. 1573-1580, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1573-1580.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Schulert, G. S., McCaffrey, R. L., Buchan, B. W., Lindemann, S. R., Hollenback, C., Jones, B. D., Allen, L.-A. H. (2009). Francisella tularensis Genes Required for Inhibition of the Neutrophil Respiratory Burst and Intramacrophage Growth Identified by Random Transposon Mutagenesis of Strain LVS. Infect. Immun. 77: 1324-1336 [Abstract] [Full Text]  
  • Ho, Y. H. S., Cai, D. T., Wang, C.-C., Huang, D., Wong, S. H. (2008). Vesicle-Associated Membrane Protein-8/Endobrevin Negatively Regulates Phagocytosis of Bacteria in Dendritic Cells. J. Immunol. 180: 3148-3157 [Abstract] [Full Text]  
  • Puri, N., Roche, P. A. (2008). Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms. Proc. Natl. Acad. Sci. USA 105: 2580-2585 [Abstract] [Full Text]  
  • Wang, C.-C., Shi, H., Guo, K., Ng, C. P., Li, J., Qi Gan, B., Chien Liew, H., Leinonen, J., Rajaniemi, H., Hong Zhou, Z., Zeng, Q., Hong, W. (2007). VAMP8/Endobrevin as a General Vesicular SNARE for Regulated Exocytosis of the Exocrine System. Mol. Biol. Cell 18: 1056-1063 [Abstract] [Full Text]  
  • Ren, Q., Barber, H. K., Crawford, G. L., Karim, Z. A., Zhao, C., Choi, W., Wang, C.-C., Hong, W., Whiteheart, S. W. (2007). Endobrevin/VAMP-8 Is the Primary v-SNARE for the Platelet Release Reaction. Mol. Biol. Cell 18: 24-33 [Abstract] [Full Text]  
  • Deak, F., Shin, O.-H., Kavalali, E. T., Sudhof, T. C. (2006). Structural determinants of synaptobrevin 2 function in synaptic vesicle fusion.. J. Neurosci. 26: 6668-6676 [Abstract] [Full Text]  
  • Proux-Gillardeaux, V., Gavard, J., Irinopoulou, T., Mege, R.-M., Galli, T. (2005). Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion. Proc. Natl. Acad. Sci. USA 102: 6362-6367 [Abstract] [Full Text]  
  • Randhawa, V. K., Thong, F. S.L., Lim, D. Y., Li, D., Garg, R. R., Rudge, R., Galli, T., Rudich, A., Klip, A. (2004). Insulin and Hypertonicity Recruit GLUT4 to the Plasma Membrane of Muscle Cells by Using N-Ethylmaleimide-sensitive Factor-dependent SNARE Mechanisms but Different v-SNAREs: Role of TI-VAMP. Mol. Biol. Cell 15: 5565-5573 [Abstract] [Full Text]  
  • Watson, R. T., Kanzaki, M., Pessin, J. E. (2004). Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes. Endocr. Rev. 25: 177-204 [Abstract] [Full Text]  
  • Schraw, T. D., Rutledge, T. W., Crawford, G. L., Bernstein, A. M., Kalen, A. L., Pessin, J. E., Whiteheart, S. W. (2003). Granule stores from cellubrevin/VAMP-3 null mouse platelets exhibit normal stimulus-induced release. Blood 102: 1716-1722 [Abstract] [Full Text]  
  • Atlashkin, V., Kreykenbohm, V., Eskelinen, E.-L., Wenzel, D., Fayyazi, A., Fischer von Mollard, G. (2003). Deletion of the SNARE vti1b in Mice Results in the Loss of a Single SNARE Partner, Syntaxin 8. Mol. Cell. Biol. 23: 5198-5207 [Abstract] [Full Text]  
  • Martinez-Arca, S., Proux-Gillardeaux, V., Alberts, P., Louvard, D., Galli, T. (2003). Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles. J. Cell Sci. 116: 2805-2816 [Abstract] [Full Text]  
  • Allen, L.-A. H., Yang, C., Pessin, J. E. (2002). Rate and extent of phagocytosis in macrophages lacking vamp3. J. Leukoc. Biol. 72: 217-221 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»