This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mingot, J. M.
Right arrow Articles by Peñalva, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mingot, J. M.
Right arrow Articles by Peñalva, M. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2001, p. 1688-1699, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1688-1699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Ambient pH Signaling Regulates Nuclear Localization of the Aspergillus nidulans PacC Transcription Factor

José M. Mingot,dagger Eduardo A. Espeso, Eliecer Díez, and Miguel Á. Peñalva*

Centro de Investigaciones Biológicas CSIC, Madrid 28006, Spain

Received 10 August 2000/Returned for modification 11 October 2000/Accepted 7 December 2000

The Aspergillus nidulans zinc finger transcription factor PacC is activated by proteolytic processing in response to ambient alkaline pH. The pH-regulated step is the transition of full-length PacC from a closed to an open, protease-accessible conformation. Here we show that in the absence of ambient pH signaling, the C-terminal negative-acting domain prevents the nuclear localization of full-length closed PacC. In contrast, the processed PacC form is almost exclusively nuclear at any ambient pH. In the presence of ambient pH signaling, the fraction of PacC that is in the open conformation but has not yet been processed localizes to the nucleus. Therefore, ambient alkaline pH leads to an increase in nuclear PacC by promoting the proteolytic elimination of the negative-acting domain to yield the processed form and by increasing the proportion of full-length protein that is in the open conformation. These findings explain why mutations resulting in commitment of PacC to processing irrespective of ambient pH lead to permanent PacC activation and alkalinity mimicry. A nuclear import signal that targets Escherichia coli beta -galactosidase to the nucleus has been located to the PacC zinc finger region. A mutation abolishing DNA binding does not prevent nuclear localization of the processed form, showing that PacC processing does not lead to nuclear localization by passive diffusion of the protein made possible by the reduction in size, followed by retention in the nucleus after DNA binding.

* Corresponding author. Mailing address: Centro de Investigaciones Biológicas CSIC, Velázquez 144, Madrid 28006, Spain. Phone: 34 91 5644562, ext. 4358. Fax: 34 91 5627518. E-mail: penalva{at}cib.csic.es.

dagger Present address: Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany.

Molecular and Cellular Biology, March 2001, p. 1688-1699, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1688-1699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pantazopoulou, A., Penalva, M. A. (2009). Organization and Dynamics of the Aspergillus nidulans Golgi during Apical Extension and Mitosis. Mol. Biol. Cell 20: 4335-4347 [Abstract] [Full Text]  
  • Calcagno-Pizarelli, A. M., Negrete-Urtasun, S., Denison, S. H., Rudnicka, J. D., Bussink, H.-J., Munera-Huertas, T., Stanton, L., Hervas-Aguilar, A., Espeso, E. A., Tilburn, J., Arst, H. N. Jr., Penalva, M. A. (2007). Establishment of the Ambient pH Signaling Complex in Aspergillus nidulans: PalI Assists Plasma Membrane Localization of PalH. Eukaryot Cell 6: 2365-2375 [Abstract] [Full Text]  
  • Baek, Y.-U., Martin, S. J., Davis, D. A. (2006). Evidence for Novel pH-Dependent Regulation of Candida albicans Rim101, a Direct Transcriptional Repressor of the Cell Wall {beta}-Glycosidase Phr2.. Eukaryot Cell 5: 1550-1559 [Abstract] [Full Text]  
  • Ramon, A. M., Fonzi, W. A. (2003). Diverged Binding Specificity of Rim101p, the Candida albicans Ortholog of PacC. Eukaryot Cell 2: 718-728 [Abstract] [Full Text]  
  • Vincent, O., Rainbow, L., Tilburn, J., Arst, H. N. Jr., Penalva, M. A. (2003). YPXL/I Is a Protein Interaction Motif Recognized by Aspergillus PalA and Its Human Homologue, AIP1/Alix. Mol. Cell. Biol. 23: 1647-1655 [Abstract] [Full Text]  
  • Lamb, T. M., Mitchell, A. P. (2003). The Transcription Factor Rim101p Governs Ion Tolerance and Cell Differentiation by Direct Repression of the Regulatory Genes NRG1 and SMP1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 23: 677-686 [Abstract] [Full Text]  
  • Penalva, M. A., Arst, H. N. Jr. (2002). Regulation of Gene Expression by Ambient pH in Filamentous Fungi and Yeasts. Microbiol. Mol. Biol. Rev. 66: 426-446 [Abstract] [Full Text]  
  • Gonzalez-Lopez, C. I., Szabo, R., Blanchin-Roland, S., Gaillardin, C. (2002). Genetic Control of Extracellular Protease Synthesis in the Yeast Yarrowia lipolytica. Genetics 160: 417-427 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»