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Molecular and Cellular Biology, March 2001, p. 1710-1718, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1710-1718.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Checkpoint Adaptation Precedes Spontaneous and Damage-Induced Genomic Instability in Yeast

David J. Galgoczy and David P. Toczyski*

Mt. Zion Cancer Research Institute, Department of Biochemistry and Biophysics, University of California, San Francisco, California 94115

Received 19 October 2000/Returned for modification 28 November 2000/Accepted 7 December 2000

Despite the fact that eukaryotic cells enlist checkpoints to block cell cycle progression when their DNA is damaged, cells still undergo frequent genetic rearrangements, both spontaneously and in response to genotoxic agents. We and others have previously characterized a phenomenon (adaptation) in which yeast cells that are arrested at a DNA damage checkpoint eventually override this arrest and reenter the cell cycle, despite the fact that they have not repaired the DNA damage that elicited the arrest. Here, we use mutants that are defective in checkpoint adaptation to show that adaptation is important for achieving the highest possible viability after exposure to DNA-damaging agents, but it also acts as an entrée into some forms of genomic instability. Specifically, the spontaneous and X-ray-induced frequencies of chromosome loss, translocations, and a repair process called break-induced replication occur at significantly reduced rates in adaptation-defective mutants. This indicates that these events occur after a cell has first arrested at the checkpoint and then adapted to that arrest. Because malignant progression frequently involves loss of genes that function in DNA repair, adaptation may promote tumorigenesis by allowing genomic instability to occur in the absence of repair.

* Corresponding author. Mailing address: 2340 Sutter St., Mt. Zion Cancer Research Institute, University of California, San Francisco, CA 94115. Phone: (415) 502-1301. Fax: (415) 502-3179. E-mail: toczyski{at}cc.ucsf.edu.

Molecular and Cellular Biology, March 2001, p. 1710-1718, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1710-1718.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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