This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gangloff, Y.-G.
Right arrow Articles by Davidson, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gangloff, Y.-G.
Right arrow Articles by Davidson, I.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2001, p. 1841-1853, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1841-1853.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Histone Folds Mediate Selective Heterodimerization of Yeast TAFII25 with TFIID Components yTAFII47 and yTAFII65 and with SAGA Component ySPT7

Yann-Gaël Gangloff,1 Steven L. Sanders,2 Christophe Romier,1 Doris Kirschner,1 P. Anthony Weil,2 Laszlo Tora,1 and Irwin Davidson1,*

Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch Cédex, C.U. de Strasbourg, France,1 and Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, Tennessee 372322

Received 25 August 2000/Returned for modification 30 September 2000/Accepted 7 December 2000

We show that the yeast TFIID (yTFIID) component yTAFII47 contains a histone fold domain (HFD) with homology to that previously described for hTAFII135. Complementation in vivo indicates that the yTAFII47 HFD is necessary and sufficient for vegetative growth. Mutation of highly conserved residues in the alpha 1 helix of the yTAFII47 HFD results in a temperature-sensitive phenotype which can be suppressed by overexpression of yTAFII25, as well as by yTAFII40, yTAFII19, and yTAFII60. In yeast two-hybrid and bacterial coexpression assays, the yTAFII47 HFD selectively heterodimerizes with yTAFII25, which we show contains an HFD with homology to the hTAFII28 family We additionally demonstrate that yTAFII65 contains a functional HFD which also selectively heterodimerizes with yTAFII25. These results reveal the existence of two novel histone-like pairs in yTFIID. The physical and genetic interactions described here show that the histone-like yTAFIIs are organized in at least two substructures within TFIID rather than in a single octamer-like structure as previously suggested. Furthermore, our results indicate that ySPT7 has an HFD homologous to that of yTAFII47 which selectively heterodimerizes with yTAFII25, defining a novel histone-like pair in the SAGA complex.

* Corresponding author. Mailing address: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, B.P. 163, 67404 Illkirch Cédex, C.U. de Strasbourg, France. Phone: 33 3 88 65 34 40 (45). Fax: 33 3 88 65 32 01. E-mail: irwin{at}titus.u-strasbg.fr.

Molecular and Cellular Biology, March 2001, p. 1841-1853, Vol. 21, No. 5
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.5.1841-1853.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Li, V. C., Davis, J. C., Lenkov, K., Bolival, B., Fuller, M. T., Petrov, D. A. (2009). Molecular Evolution of the Testis TAFs of Drosophila. Mol Biol Evol 26: 1103-1116 [Abstract] [Full Text]  
  • Liu, X., Vorontchikhina, M., Wang, Y.-L., Faiola, F., Martinez, E. (2008). STAGA Recruits Mediator to the MYC Oncoprotein To Stimulate Transcription and Cell Proliferation. Mol. Cell. Biol. 28: 108-121 [Abstract] [Full Text]  
  • Guelman, S., Suganuma, T., Florens, L., Weake, V., Swanson, S. K., Washburn, M. P., Abmayr, S. M., Workman, J. L. (2006). The Essential Gene wda Encodes a WD40 Repeat Subunit of Drosophila SAGA Required for Histone H3 Acetylation.. Mol. Cell. Biol. 26: 7178-7189 [Abstract] [Full Text]  
  • Soutoglou, E., Demeny, M. A., Scheer, E., Fienga, G., Sassone-Corsi, P., Tora, L. (2005). The Nuclear Import of TAF10 Is Regulated by One of Its Three Histone Fold Domain-Containing Interaction Partners. Mol. Cell. Biol. 25: 4092-4104 [Abstract] [Full Text]  
  • Robinson, M. M., Yatherajam, G., Ranallo, R. T., Bric, A., Paule, M. R., Stargell, L. A. (2005). Mapping and Functional Characterization of the TAF11 Interaction with TFIIA. Mol. Cell. Biol. 25: 945-957 [Abstract] [Full Text]  
  • Ingvarsdottir, K., Krogan, N. J., Emre, N. C. T., Wyce, A., Thompson, N. J., Emili, A., Hughes, T. R., Greenblatt, J. F., Berger, S. L. (2005). H2B Ubiquitin Protease Ubp8 and Sgf11 Constitute a Discrete Functional Module within the Saccharomyces cerevisiae SAGA Complex. Mol. Cell. Biol. 25: 1162-1172 [Abstract] [Full Text]  
  • Henry, K. W., Wyce, A., Lo, W.-S., Duggan, L. J., Emre, N.C. T., Kao, C.-F., Pillus, L., Shilatifard, A., Osley, M. A., Berger, S. L. (2003). Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes Dev. 17: 2648-2663 [Abstract] [Full Text]  
  • Chen, Z., Manley, J. L. (2003). In Vivo Functional Analysis of the Histone 3-like TAF9 and a TAF9-related Factor, TAF9L. J. Biol. Chem. 278: 35172-35183 [Abstract] [Full Text]  
  • Mohan, W. S. II, Scheer, E., Wendling, O., Metzger, D., Tora, L. (2003). TAF10 (TAFII30) Is Necessary for TFIID Stability and Early Embryogenesis in Mice. Mol. Cell. Biol. 23: 4307-4318 [Abstract] [Full Text]  
  • Pointud, J.-C., Mengus, G., Brancorsini, S., Monaco, L., Parvinen, M., Sassone-Corsi, P., Davidson, I. (2003). The intracellular localisation of TAF7L, a paralogue of transcription factor TFIID subunit TAF7, is developmentally regulated during male germ-cell differentiation. J. Cell Sci. 116: 1847-1858 [Abstract] [Full Text]  
  • Yatherajam, G., Zhang, L., Kraemer, S. M., Stargell, L. A. (2003). Protein-protein interaction map for yeast TFIID. Nucleic Acids Res 31: 1252-1260 [Abstract] [Full Text]  
  • Werten, S., Mitschler, A., Romier, C., Gangloff, Y.-G., Thuault, S., Davidson, I., Moras, D. (2002). Crystal Structure of a Subcomplex of Human Transcription Factor TFIID Formed by TATA Binding Protein-associated Factors hTAF4 (hTAFII135) and hTAF12 (hTAFII20). J. Biol. Chem. 277: 45502-45509 [Abstract] [Full Text]  
  • Thuault, S., Gangloff, Y.-G., Kirchner, J., Sanders, S., Werten, S., Romier, C., Weil, P. A., Davidson, I. (2002). Functional Analysis of the TFIID-specific Yeast TAF4 (yTAFII48) Reveals an Unexpected Organization of Its Histone-fold Domain. J. Biol. Chem. 277: 45510-45517 [Abstract] [Full Text]  
  • Sterner, D. E., Belotserkovskaya, R., Berger, S. L. (2002). SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription. Proc. Natl. Acad. Sci. USA 99: 11622-11627 [Abstract] [Full Text]  
  • Marc, F., Sandman, K., Lurz, R., Reeve, J. N. (2002). Archaeal Histone Tetramerization Determines DNA Affinity and the Direction of DNA Supercoiling. J. Biol. Chem. 277: 30879-30886 [Abstract] [Full Text]  
  • Sanders, S. L., Garbett, K. A., Weil, P. A. (2002). Molecular Characterization of Saccharomyces cerevisiae TFIID. Mol. Cell. Biol. 22: 6000-6013 [Abstract] [Full Text]  
  • Wu, P.-Y. J., Winston, F. (2002). Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex. Mol. Cell. Biol. 22: 5367-5379 [Abstract] [Full Text]  
  • Kirschner, D. B., vom Baur, E., Thibault, C., Sanders, S. L., Gangloff, Y.-G., Davidson, I., Weil, P. A., Tora, L. (2002). Distinct Mutations in Yeast TAFII25 Differentially Affect the Composition of TFIID and SAGA Complexes as Well as Global Gene Expression Patterns. Mol. Cell. Biol. 22: 3178-3193 [Abstract] [Full Text]  
  • Fuss, J., Linn, S. (2002). Human DNA Polymerase epsilon Colocalizes with Proliferating Cell Nuclear Antigen and DNA Replication Late, but Not Early, in S Phase. J. Biol. Chem. 277: 8658-8666 [Abstract] [Full Text]  
  • Frontini, M., Imbriano, C., diSilvio, A., Bell, B., Bogni, A., Romier, C., Moras, D., Tora, L., Davidson, I., Mantovani, R. (2002). NF-Y Recruitment of TFIID, Multiple Interactions with Histone Fold TAFIIs. J. Biol. Chem. 277: 5841-5848 [Abstract] [Full Text]  
  • Durso, R. J., Fisher, A. K., Albright-Frey, T. J., Reese, J. C. (2001). Analysis of TAF90 Mutants Displaying Allele-Specific and Broad Defects in Transcription. Mol. Cell. Biol. 21: 7331-7344 [Abstract] [Full Text]  
  • Martinez, E., Palhan, V. B., Tjernberg, A., Lymar, E. S., Gamper, A. M., Kundu, T. K., Chait, B. T., Roeder, R. G. (2001). Human STAGA Complex Is a Chromatin-Acetylating Transcription Coactivator That Interacts with Pre-mRNA Splicing and DNA Damage-Binding Factors In Vivo. Mol. Cell. Biol. 21: 6782-6795 [Abstract] [Full Text]  
  • Kirchner, J., Sanders, S. L., Klebanow, E., Weil, P. A. (2001). Molecular Genetic Dissection of TAF25, an Essential Yeast Gene Encoding a Subunit Shared by TFIID and SAGA Multiprotein Transcription Factors. Mol. Cell. Biol. 21: 6668-6680 [Abstract] [Full Text]  
  • Gangloff, Y.-G., Pointud, J.-C., Thuault, S., Carre, L., Romier, C., Muratoglu, S., Brand, M., Tora, L., Couderc, J.-L., Davidson, I. (2001). The TFIID Components Human TAFII140 and Drosophila BIP2 (TAFII155) Are Novel Metazoan Homologues of Yeast TAFII47 Containing a Histone Fold and a PHD Finger. Mol. Cell. Biol. 21: 5109-5121 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»