This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gelbart, M. E.
Right arrow Articles by Tsukiyama, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gelbart, M. E.
Right arrow Articles by Tsukiyama, T.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 2001, p. 2098-2106, Vol. 21, No. 6
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.6.2098-2106.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Interactions of Isw2 Chromatin Remodeling Complex with Nucleosomal Arrays: Analyses Using Recombinant Yeast Histones and Immobilized Templates

Marnie E. Gelbart,1 Thomas Rechsteiner,2 Timothy J. Richmond,2 and Toshio Tsukiyama1,*

Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024,1 and ETH Zurich Institut fuer Molekularbiologie und Biophysik, CH-8093 Zurich, Switzerland2

Received 11 September 2000/Returned for modification 17 October 2000/Accepted 15 December 2000

To facilitate the biochemical characterization of chromatin-associated proteins in the budding yeast Saccharomyces cerevisiae, we have developed a system to assemble nucleosomal arrays on immobilized templates using recombinant yeast core histones. This system enabled us to analyze the interaction of Isw2 ATP-dependent chromatin remodeling complex with nucleosomal arrays. We found that Isw2 complex interacts efficiently with both naked DNA and nucleosomal arrays in an ATP-independent manner, suggesting that ATP is required at steps subsequent to this physical interaction. We identified the second subunit of Isw2 complex, encoded by open reading frame YGL 133w (herein named ITC1), and found that both subunits of the complex, Isw2p and Itc1p, are essential for efficient interaction with DNA and nucleosomal arrays. Both subunits are also required for nucleosome-stimulated ATPase activity and chromatin remodeling activity of the complex. Finally, we found that ITC1 is essential for function of Isw2 complex in vivo, since isw2 and itc1 deletion mutants exhibit virtually identical phenotypes. These results demonstrate the utility of our in vitro system in studying interactions between chromatin-associated proteins and nucleosomal arrays.

* Corresponding author. Mailing address: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. North, Mail Stop A1-162, P.O. Box 19024, Seattle, WA 98109-1024. Phone: (206) 667-4996. Fax: (206) 667-6497. E-mail: ttsukiya{at}fhcrc.org.

Molecular and Cellular Biology, March 2001, p. 2098-2106, Vol. 21, No. 6
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.6.2098-2106.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kogut, I., Wang, J., Guacci, V., Mistry, R. K., Megee, P. C. (2009). The Scc2/Scc4 cohesin loader determines the distribution of cohesin on budding yeast chromosomes. Genes Dev. 23: 2345-2357 [Abstract] [Full Text]  
  • Tu, B. P., McKnight, S. L. (2009). Evidence of carbon monoxide-mediated phase advancement of the yeast metabolic cycle. Proc. Natl. Acad. Sci. USA 106: 14293-14296 [Abstract] [Full Text]  
  • Tamura, Y., Endo, T., Iijima, M., Sesaki, H. (2009). Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. JCB 185: 1029-1045 [Abstract] [Full Text]  
  • Garrenton, L. S., Braunwarth, A., Irniger, S., Hurt, E., Kunzler, M., Thorner, J. (2009). Nucleus-Specific and Cell Cycle-Regulated Degradation of Mitogen-Activated Protein Kinase Scaffold Protein Ste5 Contributes to the Control of Signaling Competence. Mol. Cell. Biol. 29: 582-601 [Abstract] [Full Text]  
  • Zill, O. A., Rine, J. (2008). Interspecies variation reveals a conserved repressor of {alpha}-specific genes in Saccharomyces yeasts. Genes Dev. 22: 1704-1716 [Abstract] [Full Text]  
  • Chen, X., Ruggiero, C., Li, S. (2007). Yeast Rpb9 Plays an Important Role in Ubiquitylation and Degradation of Rpb1 in Response to UV-Induced DNA Damage. Mol. Cell. Biol. 27: 4617-4625 [Abstract] [Full Text]  
  • Rudra, D., Mallick, J., Zhao, Y., Warner, J. R. (2007). Potential Interface between Ribosomal Protein Production and Pre-rRNA Processing. Mol. Cell. Biol. 27: 4815-4824 [Abstract] [Full Text]  
  • Fang, J., Hogan, G. J., Liang, G., Lieb, J. D., Zhang, Y. (2007). The Saccharomyces cerevisiae Histone Demethylase Jhd1 Fine-Tunes the Distribution of H3K36me2. Mol. Cell. Biol. 27: 5055-5065 [Abstract] [Full Text]  
  • Torres, M. P., Borchers, C. H. (2007). Mitotic Phosphorylation of the Anaphase-promoting Complex Inhibitory Subunit Mnd2 Is Necessary for Efficient Progression through Meiosis I. J. Biol. Chem. 282: 17351-17362 [Abstract] [Full Text]  
  • Klose, R. J., Gardner, K. E., Liang, G., Erdjument-Bromage, H., Tempst, P., Zhang, Y. (2007). Demethylation of Histone H3K36 and H3K9 by Rph1: a Vestige of an H3K9 Methylation System in Saccharomyces cerevisiae?. Mol. Cell. Biol. 27: 3951-3961 [Abstract] [Full Text]  
  • Morohashi, N., Yamamoto, Y., Kuwana, S., Morita, W., Shindo, H., Mitchell, A. P., Shimizu, M. (2006). Effect of Sequence-Directed Nucleosome Disruption on Cell-Type-Specific Repression by {alpha}2/Mcm1 in the Yeast Genome. Eukaryot Cell 5: 1925-1933 [Abstract] [Full Text]  
  • Davies, B. S. J., Rine, J. (2006). A Role for Sterol Levels in Oxygen Sensing in Saccharomyces cerevisiae. Genetics 174: 191-201 [Abstract] [Full Text]  
  • Suka, N., Nakashima, E., Shinmyozu, K., Hidaka, M., Jingami, H. (2006). The WD40-repeat protein Pwp1p associates in vivo with 25S ribosomal chromatin in a histone H4 tail-dependent manner. Nucleic Acids Res 34: 3555-3567 [Abstract] [Full Text]  
  • Chavan, M., Chen, Z., Li, G., Schindelin, H., Lennarz, W. J., Li, H. (2006). Dimeric organization of the yeast oligosaccharyl transferase complex. Proc. Natl. Acad. Sci. USA 103: 8947-8952 [Abstract] [Full Text]  
  • Babiarz, J. E., Halley, J. E., Rine, J. (2006). Telomeric heterochromatin boundaries require NuA4-dependent acetylation of histone variant H2A.Z in Saccharomy cescerevisiae.. Genes Dev. 20: 700-710 [Abstract] [Full Text]  
  • Takeshima, H., Suetake, I., Shimahara, H., Ura, K., Tate, S.-i., Tajima, S. (2006). Distinct DNA Methylation Activity of Dnmt3a and Dnmt3b towards Naked and Nucleosomal DNA.. J Biochem 139: 503-515 [Abstract] [Full Text]  
  • de Oca, R. M., Lee, K. K., Wilson, K. L. (2005). Binding of Barrier to Autointegration Factor (BAF) to Histone H3 and Selected Linker Histones Including H1.1. J. Biol. Chem. 280: 42252-42262 [Abstract] [Full Text]  
  • Fazzio, T. G., Gelbart, M. E., Tsukiyama, T. (2005). Two Distinct Mechanisms of Chromatin Interaction by the Isw2 Chromatin Remodeling Complex In Vivo. Mol. Cell. Biol. 25: 9165-9174 [Abstract] [Full Text]  
  • Li, L., Quinton, T., Miles, S., Breeden, L. L. (2005). Genetic Interactions Between Mediator and the Late G1-Specific Transcription Factor Swi6 in Saccharomyces cerevisiae. Genetics 171: 477-488 [Abstract] [Full Text]  
  • Kizer, K. O., Phatnani, H. P., Shibata, Y., Hall, H., Greenleaf, A. L., Strahl, B. D. (2005). A Novel Domain in Set2 Mediates RNA Polymerase II Interaction and Couples Histone H3 K36 Methylation with Transcript Elongation. Mol. Cell. Biol. 25: 3305-3316 [Abstract] [Full Text]  
  • Gelbart, M. E., Bachman, N., Delrow, J., Boeke, J. D., Tsukiyama, T. (2005). Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant. Genes Dev. 19: 942-954 [Abstract] [Full Text]  
  • McConnell, A. D., Gelbart, M. E., Tsukiyama, T. (2004). Histone Fold Protein Dls1p Is Required for Isw2-Dependent Chromatin Remodeling In Vivo. Mol. Cell. Biol. 24: 2605-2613 [Abstract] [Full Text]  
  • Rani, P. G., Ranish, J. A., Hahn, S. (2004). RNA Polymerase II (Pol II)-TFIIF and Pol II-Mediator Complexes: the Major Stable Pol II Complexes and Their Activity in Transcription Initiation and Reinitiation. Mol. Cell. Biol. 24: 1709-1720 [Abstract] [Full Text]  
  • Liu, Y., Kung, C., Fishburn, J., Ansari, A. Z., Shokat, K. M., Hahn, S. (2004). Two Cyclin-Dependent Kinases Promote RNA Polymerase II Transcription and Formation of the Scaffold Complex. Mol. Cell. Biol. 24: 1721-1735 [Abstract] [Full Text]  
  • Mizuguchi, G., Shen, X., Landry, J., Wu, W.-H., Sen, S., Wu, C. (2004). ATP-Driven Exchange of Histone H2AZ Variant Catalyzed by SWR1 Chromatin Remodeling Complex. Science 303: 343-348 [Abstract] [Full Text]  
  • Iida, T., Araki, H. (2004). Noncompetitive Counteractions of DNA Polymerase {varepsilon} and ISW2/yCHRAC for Epigenetic Inheritance of Telomere Position Effect in Saccharomyces cerevisiae. Mol. Cell. Biol. 24: 217-227 [Abstract] [Full Text]  
  • Robinson, K. M., Schultz, M. C. (2003). Replication-Independent Assembly of Nucleosome Arrays in a Novel Yeast Chromatin Reconstitution System Involves Antisilencing Factor Asf1p and Chromodomain Protein Chd1p. Mol. Cell. Biol. 23: 7937-7946 [Abstract] [Full Text]  
  • Hall, M. C., Torres, M. P., Schroeder, G. K., Borchers, C. H. (2003). Mnd2 and Swm1 Are Core Subunits of the Saccharomyces cerevisiae Anaphase-promoting Complex. J. Biol. Chem. 278: 16698-16705 [Abstract] [Full Text]  
  • Xiao, T., Hall, H., Kizer, K. O., Shibata, Y., Hall, M. C., Borchers, C. H., Strahl, B. D. (2003). Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast. Genes Dev. 17: 654-663 [Abstract] [Full Text]  
  • Buvelot, S., Tatsutani, S. Y., Vermaak, D., Biggins, S. (2003). The budding yeast Ipl1/Aurora protein kinase regulates mitotic spindle disassembly. JCB 160: 329-339 [Abstract] [Full Text]  
  • Ruiz, C., Escribano, V., Morgado, E., Molina, M., Mazon, M. J. (2003). Cell-type-dependent repression of yeast a-specific genes requires Itc1p, a subunit of the Isw2p-Itc1p chromatin remodelling complex. Microbiology 149: 341-351 [Abstract] [Full Text]  
  • Vary, J. C. Jr., Gangaraju, V. K., Qin, J., Landel, C. C., Kooperberg, C., Bartholomew, B., Tsukiyama, T. (2003). Yeast Isw1p Forms Two Separable Complexes In Vivo. Mol. Cell. Biol. 23: 80-91 [Abstract] [Full Text]  
  • Fyodorov, D. V., Kadonaga, J. T. (2002). Binding of Acf1 to DNA Involves a WAC Motif and Is Important for ACF-Mediated Chromatin Assembly. Mol. Cell. Biol. 22: 6344-6353 [Abstract] [Full Text]  
  • Langst, G., Becker, P. B. (2002). Nucleosome mobilization and positioning by ISWI-containing chromatin-remodeling factors. J. Cell Sci. 114: 2561-2568 [Abstract] [Full Text]  
  • Levenstein, M. E., Kadonaga, J. T. (2002). Biochemical Analysis of Chromatin Containing Recombinant Drosophila Core Histones. J. Biol. Chem. 277: 8749-8754 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»