ATF4 Degradation Relies on a Phosphorylation-Dependent Interaction with the SCF{beta}TrCP Ubiquitin Ligase

doi:10.1128/MCB.21.6.2192-2202.2001

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Molecular and Cellular Biology, March 2001, p. 2192-2202, Vol. 21, No. 6
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.6.2192-2202.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

ATF4 Degradation Relies on a Phosphorylation-Dependent Interaction with the SCF^TrCP Ubiquitin Ligase

Irina Lassot,¹ Emmanuel Ségéral,¹ Clarisse Berlioz-Torrent,¹ Herve Durand,¹ Lionel Groussin,² Tsonwin Hai,³ Richard Benarous,¹^,^* and Florence Margottin-Goguet¹

INSERM Unite 529, Interactions Moléculaires Hôte-pathogène¹ and CNRS, UPR 1524, Institut Cochin de Génétique Moléculaire,² 75014 Paris, France, and Department of Molecular and Cellular Biochemistry and Neurobiotechnology Center, Ohio State University, Columbus, Ohio³

Received 7 August 2000/Returned for modification 19 September 2000/Accepted 12 December 2000

The ubiquitin-proteasome pathway regulates gene expression through protein degradation. Here we show that the F-box protein $beta$ TrCP, the receptor component of the SCF E3 ubiquitin ligase responsiblefor I $kappa$ B $alpha$ and $beta$ -catenin degradation, is colocalized in the nucleuswith ATF4, a member of the ATF-CREB bZIP family of transcriptionfactors, and controls its stability. Association between the twoproteins depends on ATF4 phosphorylation and on ATF4 serine residue219 present in the context of DSGXXXS, which is similar but notidentical to the motif found in other substrates of $beta$ TrCP. ATF4ubiquitination in HeLa cells is enhanced in the presence of $beta$ TrCP.The F-box-deleted $beta$ TrCP protein behaves as a negative transdominantmutant that inhibits ATF4 ubiquitination and degradation and,subsequently, enhances its activity in cyclic AMP-mediated transcription.ATF4 represents a novel substrate for the SCF^TrCP complex, which is the first mammalian E3 ubiquitin ligase identifiedso far for the control of the degradation of a bZIP transcriptionfactor.

^* Corresponding author. Mailing address: INSERM Unite 529, ICGM, 24 rue de Faubourg Saint Jacques, 75014 Paris, France. Phone:33 1 44 41 25 65. Fax: 33 1 44 41 23 99. E-mail: benarous{at}cochin.inserm.fr.

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ATF4 Degradation Relies on a Phosphorylation-Dependent Interaction with the SCFTrCP Ubiquitin Ligase

ATF4 Degradation Relies on a Phosphorylation-Dependent Interaction with the SCF^TrCP Ubiquitin Ligase