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Molecular and Cellular Biology, April 2001, p. 2349-2358, Vol. 21, No. 7
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.7.2349-2358.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel Function of Rad27 (FEN-1) in Restricting Short-Sequence Recombination

M. Cristina Negritto,1 Junzhuan Qiu,2 Dawn O. Ratay,1 Binghui Shen,2 and Adam M. Bailis1,*

Department of Molecular Biology, Beckman Research Institute,1 and Department of Cell and Tumor Biology, City of Hope National Medical Center,2 Duarte, California 91010-0269

Received 1 December 2000/Returned for modification 6 January 2001/Accepted 15 January 2001

Saccharomyces cerevisiae mutants lacking the structure-specific nuclease Rad27 display an enhancement in recombination that increases as sequence length decreases, suggesting that Rad27 preferentially restricts recombination between short sequences. Since wild-type alleles of both RAD27 and its human homologue FEN1 complement the elevated short-sequence recombination (SSR) phenotype of a rad27-null mutant, this function may be conserved from yeast to humans. Furthermore, mutant Rad27 and FEN-1 enzymes with partial flap endonuclease activity but without nick-specific exonuclease activity partially complement the SSR phenotype of the rad27-null mutant. This suggests that the endonuclease activity of Rad27 (FEN-1) plays a role in limiting recombination between short sequences in eukaryotic cells.

* Corresponding author. Mailing address: Department of Molecular Biology, Beckman Institute of the City of Hope, 1450 E. Duarte Rd., Duarte, CA 91010-0269. Phone: (626) 359-8111, ext. 64031. Fax: (626) 301-8271. E-mail: abailis{at}coh.org.

Molecular and Cellular Biology, April 2001, p. 2349-2358, Vol. 21, No. 7
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.7.2349-2358.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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