Previous Article | Next Article ![]()
Molecular and Cellular Biology, April 2001, p. 2475-2484, Vol. 21, No. 7
Department of Molecular Biology, Graduate
School of Science, Nagoya University, and CREST, Japan Science and
Technology Corporation, Chikusa-ku, Nagoya 464-8602, Japan,1 and Departments of
Immunology2 and Molecular
Biology,3 Lerner Research Institute, Cleveland
Clinic Foundation, Cleveland, Ohio 44195
Received 6 September 2000/Returned for modification 23 October
2000/Accepted 12 January 2001
Interleukin-1 (IL-1) is a proinflammatory cytokine that recognizes
a surface receptor complex and generates multiple cellular responses.
IL-1 stimulation activates the mitogen-activated protein kinase kinase
kinase TAK1, which in turn mediates activation of c-Jun N-terminal
kinase and NF-
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.7.2475-2484.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Interleukin-1 (IL-1) Receptor-Associated Kinase
Leads to Activation of TAK1 by Inducing TAB2 Translocation in the
IL-1 Signaling Pathway
B. TAB2 has previously been shown to interact with
both TAK1 and TRAF6 and promote their association, thereby triggering
subsequent IL-1 signaling events. The serine/threonine kinase IL-1
receptor-associated kinase (IRAK) also plays a role in IL-1 signaling,
being recruited to the IL-1 receptor complex early in the signal
cascade. In this report, we investigate the role of IRAK in the
activation of TAK1. Genetic analysis reveals that IRAK is required for
IL-1-induced activation of TAK1. We show that IL-1 stimulation induces
the rapid but transient association of IRAK, TRAF6, TAB2, and TAK1.
TAB2 is recruited to this complex following translocation from the
membrane to the cytosol upon IL-1 stimulation. In IRAK-deficient cells,
TAB2 translocation and its association with TRAF6 are abolished. These
results suggest that IRAK regulates the redistribution of TAB2 upon
IL-1 stimulation and facilitates the formation of a TRAF6-TAB2-TAK1
complex. Formation of this complex is an essential step in the
activation of TAK1 in the IL-1 signaling pathway.
*
Corresponding author. Mailing address: Department of
Molecular Biology, Graduate School of Science, Nagoya University,
Chikusa-ku, Nagoya 464-8602, Japan. Phone: 81-52-789-3000. Fax:
81-52-789-2589. E-mail:
g44177a{at}nucc.cc.nagoya-u.ac.jp.
This article has been cited by other articles:
| J. Bacteriol. | J. Virol. | Eukaryot. Cell |
|---|
| Microbiol. Mol. Biol. Rev. | Clin. Vaccine Immunol. | All ASM Journals |
|---|