This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kadosh, D.
Right arrow Articles by Johnson, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kadosh, D.
Right arrow Articles by Johnson, A. D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2001, p. 2496-2505, Vol. 21, No. 7
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.7.2496-2505.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rfg1, a Protein Related to the Saccharomyces cerevisiae Hypoxic Regulator Rox1, Controls Filamentous Growth and Virulence in Candida albicans

David Kadosh and Alexander D. Johnson*

Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California 94143

Received 9 October 2000/Returned for modification 30 November 2000/Accepted 18 December 2000

Candida albicans, the major fungal pathogen in humans, can undergo a reversible transition from ellipsoidal single cells (blastospores) to filaments composed of elongated cells attached end to end. This transition is thought to allow for rapid colonization of host tissues, facilitating the spread of infection. Here, we report the identification of Rfg1, a transcriptional regulator that controls filamentous growth of C. albicans in an environment-dependent manner. Rfg1 is important for virulence of C. albicans in a mouse model and is shown to control a number of genes that have been implicated in this process. The closest relative to Rfg1 in Saccharomyces cerevisiae is Rox1, a key repressor of hypoxic genes. However, Rfg1 does not appear to play a role in the regulation of hypoxic genes in C. albicans. These results demonstrate that a regulatory protein that controls the hypoxic response in S. cerevisiae controls filamentous growth and virulence in C. albicans. The observations described in this paper raise new and intriguing questions about the evolutionary relationship between these processes.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of California, San Francisco, 513 Parnassus Ave., Box 0414, San Francisco, CA 94143. Phone: (415) 476-8783. Fax: (415) 476-0939. E-mail: ajohnson{at}socrates.ucsf.edu.

Molecular and Cellular Biology, April 2001, p. 2496-2505, Vol. 21, No. 7
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.7.2496-2505.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hao, B., Clancy, C. J., Cheng, S., Raman, S. B., Iczkowski, K. A., Nguyen, M. H. (2009). Candida albicans RFX2 Encodes a DNA Binding Protein Involved in DNA Damage Responses, Morphogenesis, and Virulence. Eukaryot Cell 8: 627-639 [Abstract] [Full Text]  
  • Shen, J., Cowen, L. E., Griffin, A. M., Chan, L., Kohler, J. R. (2008). The Candida albicans pescadillo homolog is required for normal hypha-to-yeast morphogenesis and yeast proliferation. Proc. Natl. Acad. Sci. USA 105: 20918-20923 [Abstract] [Full Text]  
  • Ramsdale, M., Selway, L., Stead, D., Walker, J., Yin, Z., Nicholls, S. M., Crowe, J., Sheils, E. M., Brown, A. J.P. (2008). MNL1 Regulates Weak Acid-induced Stress Responses of the Fungal Pathogen Candida albicans. Mol. Biol. Cell 19: 4393-4403 [Abstract] [Full Text]  
  • Rauceo, J. M., Blankenship, J. R., Fanning, S., Hamaker, J. J., Deneault, J.-S., Smith, F. J., Nantel, A., Mitchell, A. P. (2008). Regulation of the Candida albicans Cell Wall Damage Response by Transcription Factor Sko1 and PAS Kinase Psk1. Mol. Biol. Cell 19: 2741-2751 [Abstract] [Full Text]  
  • Kebaara, B. W., Langford, M. L., Navarathna, D. H. M. L. P., Dumitru, R., Nickerson, K. W., Atkin, A. L. (2008). Candida albicans Tup1 Is Involved in Farnesol-Mediated Inhibition of Filamentous-Growth Induction. Eukaryot Cell 7: 980-987 [Abstract] [Full Text]  
  • Goyard, S., Knechtle, P., Chauvel, M., Mallet, A., Prevost, M.-C., Proux, C., Coppee, J.-Y., Schwartz, P., Dromer, F., Park, H., Filler, S. G., Janbon, G., d'Enfert, C. (2008). The Yak1 Kinase Is Involved in the Initiation and Maintenance of Hyphal Growth in Candida albicans. Mol. Biol. Cell 19: 2251-2266 [Abstract] [Full Text]  
  • Banerjee, M., Thompson, D. S., Lazzell, A., Carlisle, P. L., Pierce, C., Monteagudo, C., Lopez-Ribot, J. L., Kadosh, D. (2008). UME6, a Novel Filament-specific Regulator of Candida albicans Hyphal Extension and Virulence. Mol. Biol. Cell 19: 1354-1365 [Abstract] [Full Text]  
  • Agarwal, A. K., Xu, T., Jacob, M. R., Feng, Q., Lorenz, M. C., Walker, L. A., Clark, A. M. (2008). Role of Heme in the Antifungal Activity of the Azaoxoaporphine Alkaloid Sampangine. Eukaryot Cell 7: 387-400 [Abstract] [Full Text]  
  • Biswas, S., Van Dijck, P., Datta, A. (2007). Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans. Microbiol. Mol. Biol. Rev. 71: 348-376 [Abstract] [Full Text]  
  • Argimon, S., Wishart, J. A., Leng, R., Macaskill, S., Mavor, A., Alexandris, T., Nicholls, S., Knight, A. W., Enjalbert, B., Walmsley, R., Odds, F. C., Gow, N. A. R., Brown, A. J. P. (2007). Developmental Regulation of an Adhesin Gene during Cellular Morphogenesis in the Fungal Pathogen Candida albicans. Eukaryot Cell 6: 682-692 [Abstract] [Full Text]  
  • Martchenko, M., Levitin, A., Whiteway, M. (2007). Transcriptional Activation Domains of the Candida albicans Gcn4p and Gal4p Homologs. Eukaryot Cell 6: 291-301 [Abstract] [Full Text]  
  • Mulhern, S. M., Logue, M. E., Butler, G. (2006). Candida albicans Transcription Factor Ace2 Regulates Metabolism and Is Required for Filamentation in Hypoxic Conditions. Eukaryot Cell 5: 2001-2013 [Abstract] [Full Text]  
  • Kaneko, A., Umeyama, T., Utena-Abe, Y., Yamagoe, S., Niimi, M., Uehara, Y. (2006). Tcc1p, a Novel Protein Containing the Tetratricopeptide Repeat Motif, Interacts with Tup1p To Regulate Morphological Transition and Virulence in Candida albicans. Eukaryot Cell 5: 1894-1905 [Abstract] [Full Text]  
  • Nickerson, K. W., Atkin, A. L., Hornby, J. M. (2006). Quorum sensing in dimorphic fungi: farnesol and beyond.. Appl. Environ. Microbiol. 72: 3805-3813 [Full Text]  
  • Wolfe, K. H (2006). Comparative genomics and genome evolution in yeasts. Phil Trans R Soc B 361: 403-412 [Abstract] [Full Text]  
  • Tournu, H., Tripathi, G., Bertram, G., Macaskill, S., Mavor, A., Walker, L., Odds, F. C., Gow, N. A. R., Brown, A. J. P. (2005). Global Role of the Protein Kinase Gcn2 in the Human Pathogen Candida albicans. Eukaryot Cell 4: 1687-1696 [Abstract] [Full Text]  
  • Hromatka, B. S., Noble, S. M., Johnson, A. D. (2005). Transcriptional Response of Candida albicans to Nitric Oxide and the Role of the YHB1 Gene in Nitrosative Stress and Virulence. Mol. Biol. Cell 16: 4814-4826 [Abstract] [Full Text]  
  • Kadosh, D., Johnson, A. D. (2005). Induction of the Candida albicans Filamentous Growth Program by Relief of Transcriptional Repression: A Genome-wide Analysis. Mol. Biol. Cell 16: 2903-2912 [Abstract] [Full Text]  
  • Bassilana, M., Hopkins, J., Arkowitz, R. A. (2005). Regulation of the Cdc42/Cdc24 GTPase Module during Candida albicans Hyphal Growth. Eukaryot Cell 4: 588-603 [Abstract] [Full Text]  
  • Nicholls, S., Straffon, M., Enjalbert, B., Nantel, A., Macaskill, S., Whiteway, M., Brown, A. J. P. (2004). Msn2- and Msn4-Like Transcription Factors Play No Obvious Roles in the Stress Responses of the Fungal Pathogen Candida albicans. Eukaryot Cell 3: 1111-1123 [Abstract] [Full Text]  
  • Staab, J. F., Bahn, Y.-S., Tai, C.-H., Cook, P. F., Sundstrom, P. (2004). Expression of Transglutaminase Substrate Activity on Candida albicans Germ Tubes through a Coiled, Disulfide-bonded N-terminal Domain of Hwp1 Requires C-terminal Glycosylphosphatidylinositol Modification. J. Biol. Chem. 279: 40737-40747 [Abstract] [Full Text]  
  • Smith, D. A., Nicholls, S., Morgan, B. A., Brown, A. J.P., Quinn, J. (2004). A Conserved Stress-activated Protein Kinase Regulates a Core Stress Response in the Human Pathogen Candida albicans. Mol. Biol. Cell 15: 4179-4190 [Abstract] [Full Text]  
  • Brand, A., MacCallum, D. M., Brown, A. J. P., Gow, N. A. R., Odds, F. C. (2004). Ectopic Expression of URA3 Can Influence the Virulence Phenotypes and Proteome of Candida albicans but Can Be Overcome by Targeted Reintegration of URA3 at the RPS10 Locus. Eukaryot Cell 3: 900-909 [Abstract] [Full Text]  
  • Krueger, K. E., Ghosh, A. K., Krom, B. P., Cihlar, R. L. (2004). Deletion of the NOT4 gene impairs hyphal development and pathogenicity in Candida albicans. Microbiology 150: 229-240 [Abstract] [Full Text]  
  • Staab, J. F., Bahn, Y.-S., Sundstrom, P. (2003). Integrative, multifunctional plasmids for hypha-specific or constitutive expression of green fluorescent protein in Candida albicans. Microbiology 149: 2977-2986 [Abstract] [Full Text]  
  • Saville, S. P., Lazzell, A. L., Monteagudo, C., Lopez-Ribot, J. L. (2003). Engineered Control of Cell Morphology In Vivo Reveals Distinct Roles for Yeast and Filamentous Forms of Candida albicans during Infection. Eukaryot Cell 2: 1053-1060 [Abstract] [Full Text]  
  • Enjalbert, B., Nantel, A., Whiteway, M. (2003). Stress-induced Gene Expression in Candida albicans: Absence of a General Stress Response. Mol. Biol. Cell 14: 1460-1467 [Abstract] [Full Text]  
  • Henry, K. W., Nickels, J. T., Edlind, T. D. (2002). ROX1 and ERG Regulation in Saccharomyces cerevisiae: Implications for Antifungal Susceptibility. Eukaryot Cell 1: 1041-1044 [Abstract] [Full Text]  
  • Hu, C.-J., Bai, C., Zheng, X.-D., Wang, Y.-M., Wang, Y. (2002). Characterization and Functional Analysis of the Siderophore-Iron Transporter CaArn1p in Candida albicans. J. Biol. Chem. 277: 30598-30605 [Abstract] [Full Text]  
  • Laprade, L., Boyartchuk, V. L., Dietrich, W. F., Winston, F. (2002). Spt3 Plays Opposite Roles in Filamentous Growth in Saccharomyces cerevisiae and Candida albicans and Is Required for C. albicans Virulence. Genetics 161: 509-519 [Abstract] [Full Text]  
  • Lane, S., Birse, C., Zhou, S., Matson, R., Liu, H. (2001). DNA Array Studies Demonstrate Convergent Regulation of Virulence Factors by Cph1, Cph2, and Efg1 in Candida albicans. J. Biol. Chem. 276: 48988-48996 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»