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Molecular and Cellular Biology, April 2001, p. 2790-2801, Vol. 21, No. 8
Department of Microbiology and Molecular
Genetics, UMDNJ-New Jersey Medical School, Newark, New Jersey 07103
Received 19 October 2000/Returned for modification 4 December
2000/Accepted 25 January 2001
While many of the proteins involved in the initiation of DNA
replication are conserved between yeasts and metazoans, the structure of the replication origins themselves has appeared to be different. As
typified by ARS1, replication origins in
Saccharomyces cerevisiae are <150 bp long and have a
simple modular structure, consisting of a single binding site for
the origin recognition complex, the replication initiator protein, and
one or more accessory sequences. DNA replication initiates from a
discrete site. While the important sequences are currently less well
defined, metazoan origins appear to be different. These origins are
large and appear to be composed of multiple, redundant elements, and
replication initiates throughout zones as large as 55 kb. In this
report, we characterize two S. cerevisiae replication
origins, ARS101 and ARS310, which differ from
the paradigm. These origins contain multiple, redundant binding sites for the origin recognition complex. Each binding site must be
altered to abolish origin function, while the alteration of a single
binding site is sufficient to inactivate ARS1. This
redundant structure may be similar to that seen in metazoan origins.
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.8.2790-2801.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Two Compound Replication Origins in Saccharomyces
cerevisiae Contain Redundant Origin Recognition Complex
Binding Sites
*
Corresponding author. Mailing address: Department of
Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 185 South Orange Ave., Newark, NJ 07103. Phone: (973) 972-4227. Fax:
(973) 972-3644. E-mail: newlon{at}umdnj.edu.
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