This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fontijn, R. D.
Right arrow Articles by Horrevoets, A. J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fontijn, R. D.
Right arrow Articles by Horrevoets, A. J. G.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 2001, p. 2944-2955, Vol. 21, No. 8
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.8.2944-2955.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Human Kinesin-Like Protein RB6K Is under Tight Cell Cycle Control and Is Essential for Cytokinesis

Ruud D. Fontijn,1 Bruno Goud,2 Arnaud Echard,2 Florence Jollivet,2 Jan van Marle,3 Hans Pannekoek,1 and Anton J. G. Horrevoets1,*

Departments of Biochemistry1 and Electron Microscopy,3 Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, and UMR CNRS 144, Institut Curie, Paris, France2

Received 21 September 2000/Returned for modification 25 October 2000/Accepted 10 January 2001

Several members of the kinesin superfamily are known to play a prominent role in the motor-driven transport processes that occur in mitotic cells. Here we describe a new mitotic human kinesin-like protein, RB6K (Rabkinesin 6), distantly related to MKLP-1. Expression of RB6K is regulated during the cell cycle at both the mRNA and protein level and, similar to cyclin B, shows a maximum during M phase. Isolation of the RB6K promoter allowed identification of a CDE-CHR element and promoter activity was shown to be maximal during M phase. Immunofluorescence microscopy using antibodies raised against RB6K showed a weak signal in interphase Golgi but a 10-fold higher signal in prophase nuclei. During M phase, the newly synthesized RB6K does not colocalise with Rab6. In later stages of mitosis RB6K localized to the spindle midzone and appeared on the midbodies during cytokinesis. The functional significance of this localization during M phase was revealed by antibody microinjection studies which resulted exclusively in binucleate cells, showing a complete failure of cytokinesis. These results substantiate a crucial role for RB6K in late anaphase B and/or cytokinesis, clearly distinct from the role of MKLP-1.

* Corresponding author. Mailing address: Department of Biochemistry, Academic Medical Center K1-160, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. Phone: 31-20-5665124. Fax: 31-20-6915519. E-mail: a.j.horrevoets{at}amc.uva.nl.

Molecular and Cellular Biology, April 2001, p. 2944-2955, Vol. 21, No. 8
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.8.2944-2955.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Johansen, J. N., Chow, C.-M., Moore, I., Hawes, C. (2009). AtRAB-H1b and AtRAB-H1c GTPases, homologues of the yeast Ypt6, target reporter proteins to the Golgi when expressed in Nicotiana tabacum and Arabidopsis thaliana. J Exp Bot 0: erp153v2-erp153 [Abstract] [Full Text]  
  • Seidl, C., Port, M., Gilbertz, K.-P., Morgenstern, A., Bruchertseifer, F., Schwaiger, M., Roper, B., Senekowitsch-Schmidtke, R., Abend, M. (2007). 213Bi-induced death of HSC45-M2 gastric cancer cells is characterized by G2 arrest and up-regulation of genes known to prevent apoptosis but induce necrosis and mitotic catastrophe. Molecular Cancer Therapeutics 6: 2346-2359 [Abstract] [Full Text]  
  • Fledderus, J. O., van Thienen, J. V., Boon, R. A., Dekker, R. J., Rohlena, J., Volger, O. L., Bijnens, A.-P. J. J., Daemen, M. J. A. P., Kuiper, J., van Berkel, T. J. C., Pannekoek, H., Horrevoets, A. J. G. (2007). Prolonged shear stress and KLF2 suppress constitutive proinflammatory transcription through inhibition of ATF2. Blood 109: 4249-4257 [Abstract] [Full Text]  
  • Myslinski, E., Gerard, M.-A., Krol, A., Carbon, P. (2007). Transcription of the human cell cycle regulated BUB1B gene requires hStaf/ZNF143. Nucleic Acids Res 35: 3453-3464 [Abstract] [Full Text]  
  • Yu, T., Peng, Y.-C., Androphy, E. J. (2007). Mitotic Kinesin-Like Protein 2 Binds and Colocalizes with Papillomavirus E2 during Mitosis. J. Virol. 81: 1736-1745 [Abstract] [Full Text]  
  • Cesario, J. M., Jang, J. K., Redding, B., Shah, N., Rahman, T., McKim, K. S. (2006). Kinesin 6 family member Subito participates in mitotic spindle assembly and interacts with mitotic regulators. J. Cell Sci. 119: 4770-4780 [Abstract] [Full Text]  
  • Zhao, W.-m., Seki, A., Fang, G. (2006). Cep55, a Microtubule-bundling Protein, Associates with Centralspindlin to Control the Midbody Integrity and Cell Abscission during Cytokinesis. Mol. Biol. Cell 17: 3881-3896 [Abstract] [Full Text]  
  • Dekker, R. J., Boon, R. A., Rondaij, M. G., Kragt, A., Volger, O. L., Elderkamp, Y. W., Meijers, J. C. M., Voorberg, J., Pannekoek, H., Horrevoets, A. J. G. (2006). KLF2 provokes a gene expression pattern that establishes functional quiescent differentiation of the endothelium. Blood 107: 4354-4363 [Abstract] [Full Text]  
  • Carleton, M., Mao, M., Biery, M., Warrener, P., Kim, S., Buser, C., Marshall, C. G., Fernandes, C., Annis, J., Linsley, P. S. (2006). RNA Interference-Mediated Silencing of Mitotic Kinesin KIF14 Disrupts Cell Cycle Progression and Induces Cytokinesis Failure. Mol. Cell. Biol. 26: 3853-3863 [Abstract] [Full Text]  
  • Schmidt, S., Rainer, J., Riml, S., Ploner, C., Jesacher, S., Achmuller, C., Presul, E., Skvortsov, S., Crazzolara, R., Fiegl, M., Raivio, T., Janne, O. A., Geley, S., Meister, B., Kofler, R. (2006). Identification of glucocorticoid-response genes in children with acute lymphoblastic leukemia. Blood 107: 2061-2069 [Abstract] [Full Text]  
  • Jang, J. K., Rahman, T., McKim, K. S. (2005). The Kinesinlike Protein Subito Contributes to Central Spindle Assembly and Organization of the Meiotic Spindle in Drosophila Oocytes. Mol. Biol. Cell 16: 4684-4694 [Abstract] [Full Text]  
  • Zhu, C., Zhao, J., Bibikova, M., Leverson, J. D., Bossy-Wetzel, E., Fan, J.-B., Abraham, R. T., Jiang, W. (2005). Functional Analysis of Human Microtubule-based Motor Proteins, the Kinesins and Dyneins, in Mitosis/Cytokinesis Using RNA Interference. Mol. Biol. Cell 16: 3187-3199 [Abstract] [Full Text]  
  • Wonsey, D. R., Follettie, M. T. (2005). Loss of the Forkhead Transcription Factor FoxM1 Causes Centrosome Amplification and Mitotic Catastrophe. Cancer Res. 65: 5181-5189 [Abstract] [Full Text]  
  • Jackson, M. W., Agarwal, M. K., Yang, J., Bruss, P., Uchiumi, T., Agarwal, M. L., Stark, G. R., Taylor,, W. R. (2005). p130/p107/p105Rb-dependent transcriptional repression during DNA-damage-induced cell-cycle exit at G2. J. Cell Sci. 118: 1821-1832 [Abstract] [Full Text]  
  • D'Avino, P. P., Savoian, M. S., Glover, D. M. (2005). Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy. J. Cell Sci. 118: 1549-1558 [Abstract] [Full Text]  
  • Barr, F. A., Egerer, J. (2005). Golgi positioning: are we looking at the right MAP?. JCB 168: 993-998 [Abstract] [Full Text]  
  • Mollinari, C., Kleman, J.-P., Saoudi, Y., Jablonski, S. A., Perard, J., Yen, T. J., Margolis, R. L. (2005). Ablation of PRC1 by Small Interfering RNA Demonstrates that Cytokinetic Abscission Requires a Central Spindle Bundle in Mammalian Cells, whereas Completion of Furrowing Does Not. Mol. Biol. Cell 16: 1043-1055 [Abstract] [Full Text]  
  • Taniuchi, K., Nakagawa, H., Nakamura, T., Eguchi, H., Ohigashi, H., Ishikawa, O., Katagiri, T., Nakamura, Y. (2005). Down-regulation of RAB6KIFL/KIF20A, a Kinesin Involved with Membrane Trafficking of Discs Large Homologue 5, Can Attenuate Growth of Pancreatic Cancer Cell. Cancer Res. 65: 105-112 [Abstract] [Full Text]  
  • Young, J., Stauber, T., del Nery, E., Vernos, I., Pepperkok, R., Nilsson, T. (2005). Regulation of Microtubule-dependent Recycling at the Trans-Golgi Network by Rab6A and Rab6A'. Mol. Biol. Cell 16: 162-177 [Abstract] [Full Text]  
  • Lele, K. M., Wolgemuth, D. J. (2004). Distinct Regions of the Mouse Cyclin A1 Gene, Ccna1, Confer Male Germ-Cell Specific Expression and Enhancer Function. Biol. Reprod. 71: 1340-1347 [Abstract] [Full Text]  
  • DeBonis, S., Skoufias, D. A., Lebeau, L., Lopez, R., Robin, G., Margolis, R. L., Wade, R. H., Kozielski, F. (2004). In vitro screening for inhibitors of the human mitotic kinesin Eg5 with antimitotic and antitumor activities. Molecular Cancer Therapeutics 3: 1079-1090 [Abstract] [Full Text]  
  • Gruneberg, U., Neef, R., Honda, R., Nigg, E. A., Barr, F. A. (2004). Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2. JCB 166: 167-172 [Abstract] [Full Text]  
  • Harvey, S. A. K., Anderson, S. C., SundarRaj, N. (2004). Downstream Effects of ROCK Signaling in Cultured Human Corneal Stromal Cells: Microarray Analysis of Gene Expression. IOVS 45: 2168-2176 [Abstract] [Full Text]  
  • Vanstraelen, M., Torres Acosta, J. A., De Veylder, L., Inze, D., Geelen, D. (2004). A Plant-Specific Subclass of C-Terminal Kinesins Contains a Conserved A-Type Cyclin-Dependent Kinase Site Implicated in Folding and Dimerization. Plant Physiol. 135: 1417-1429 [Abstract] [Full Text]  
  • Fan, J., Beck, K. A. (2004). A role for the spectrin superfamily member Syne-1 and kinesin II in cytokinesis. J. Cell Sci. 117: 619-629 [Abstract] [Full Text]  
  • Neef, R., Preisinger, C., Sutcliffe, J., Kopajtich, R., Nigg, E. A., Mayer, T. U., Barr, F. A. (2003). Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis. JCB 162: 863-876 [Abstract] [Full Text]  
  • Abaza, A., Soleilhac, J.-M., Westendorf, J., Piel, M., Crevel, I., Roux, A., Pirollet, F. (2003). M Phase Phosphoprotein 1 Is a Human Plus-end-directed Kinesin-related Protein Required for Cytokinesis. J. Biol. Chem. 278: 27844-27852 [Abstract] [Full Text]  
  • Stedman, T. T., Sussmann, A. R., Joiner, K. A. (2003). Toxoplasma gondii Rab6 Mediates a Retrograde Pathway for Sorting of Constitutively Secreted Proteins to the Golgi Complex. J. Biol. Chem. 278: 5433-5443 [Abstract] [Full Text]  
  • Dekker, R. J., van Soest, S., Fontijn, R. D., Salamanca, S., de Groot, P. G., VanBavel, E., Pannekoek, H., Horrevoets, A. J. G. (2002). Prolonged fluid shear stress induces a distinct set of endothelial cell genes, most specifically lung Kruppel-like factor (KLF2). Blood 100: 1689-1698 [Abstract] [Full Text]  
  • Whitfield, M. L., Sherlock, G., Saldanha, A. J., Murray, J. I., Ball, C. A., Alexander, K. E., Matese, J. C., Perou, C. M., Hurt, M. M., Brown, P. O., Botstein, D. (2002). Identification of Genes Periodically Expressed in the Human Cell Cycle and Their Expression in Tumors. Mol. Biol. Cell 13: 1977-2000 [Abstract] [Full Text]  
  • Wu, X., Wang, F., Rao, K., Sellers, J. R., Hammer, J. A. III (2002). Rab27a Is an Essential Component of Melanosome Receptor for Myosin Va. Mol. Biol. Cell 13: 1735-1749 [Abstract] [Full Text]  
  • Giunta, K. L., Jang, J. K., Manheim, E. A., Subramanian, G., McKim, K. S. (2002). subito Encodes a Kinesin-like Protein Required for Meiotic Spindle Pole Formation in Drosophila melanogaster. Genetics 160: 1489-1501 [Abstract] [Full Text]  
  • Tanaka, M., Ueda, A., Kanamori, H., Ideguchi, H., Yang, J., Kitajima, S., Ishigatsubo, Y. (2002). Cell-cycle-dependent Regulation of Human aurora A Transcription Is Mediated by Periodic Repression of E4TF1. J. Biol. Chem. 277: 10719-10726 [Abstract] [Full Text]  
  • Mallard, F., Tang, B. L., Galli, T., Tenza, D., Saint-Pol, A., Yue, X., Antony, C., Hong, W., Goud, B., Johannes, L. (2002). Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform. JCB 156: 653-664 [Abstract] [Full Text]  
  • Kamimoto, T., Zama, T., Aoki, R., Muro, Y., Hagiwara, M. (2001). Identification of a Novel Kinesin-related Protein, KRMP1, as a Target for Mitotic Peptidyl-prolyl Isomerase Pin1. J. Biol. Chem. 276: 37520-37528 [Abstract] [Full Text]  
  • Mallard, F., Tang, B. L., Galli, T., Tenza, D., Saint-Pol, A., Yue, X., Antony, C., Hong, W., Goud, B., Johannes, L. (2002). Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform. JCB 156: 653-664 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»