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Molecular and Cellular Biology, May 2001, p. 2991-3000, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.2991-3000.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Liver X Receptor-Retinoid X Receptor as an Activator of the Sterol Regulatory Element-Binding Protein 1c Gene Promoter

Tomohiro Yoshikawa,1 Hitoshi Shimano,2,* Michiyo Amemiya-Kudo,1 Naoya Yahagi,1 Alyssa H. Hasty,1 Takashi Matsuzaka,2 Hiroaki Okazaki,1 Yoshiaki Tamura,1 Yoko Iizuka,1 Ken Ohashi,1 Jun-Ichi Osuga,1 Kenji Harada,1 Takanari Gotoda,1 Satoshi Kimura,1 Shun Ishibashi,1 and Nobuhiro Yamada2

Department of Metabolic Diseases, University of Tokyo, Bunkyo-ku, Tokyo 113-8655,1 and Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, Ibaraki 305-8575,2 Japan

Received 23 October 2000/Returned for modification 19 December 2000/Accepted 9 February 2001

In an attempt to identify transcription factors which activate sterol-regulatory element-binding protein 1c (SREBP-1c) transcription, we screened an expression cDNA library from adipose tissue of SREBP-1 knockout mice using a reporter gene containing the 2.6-kb mouse SREBP-1 gene promoter. We cloned and identified the oxysterol receptors liver X receptor (LXRalpha ) and LXRbeta as strong activators of the mouse SREBP-1c promoter. In the transfection studies, expression of either LXRalpha or -beta activated the SREBP-1c promoter-luciferase gene in a dose-dependent manner. Deletion and mutation studies, as well as gel mobility shift assays, located an LXR response element complex consisting of two new LXR-binding motifs which showed high similarity to an LXR response element recently found in the ABC1 gene promoter, a reverse cholesterol transporter. Addition of an LXR ligand, 22(R)-hydroxycholesterol, increased the promoter activity. Coexpression of retinoid X receptor (RXR), a heterodimeric partner, and its ligand 9-cis-retinoic acid also synergistically activated the SREBP-1c promoter. In HepG2 cells, SREBP-1c mRNA and precursor protein levels were induced by treatment with 22(R)-hydroxycholesterol and 9-cis-retinoic acid, confirming that endogenous LXR-RXR activation can induce endogenous SREBP-1c expression. The activation of SREBP-1c by LXR is associated with a slight increase in nuclear SREBP-1c, resulting in activation of the gene for fatty acid synthase, one of its downstream genes, as measured by the luciferase assay. These data demonstrate that LXR-RXR can modify the expression of genes for lipogenic enzymes by regulating SREBP-1c expression, providing a novel link between fatty acid and cholesterol metabolism.


* Corresponding author. Mailing address: Department of Internal Medicine, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan. Phone and fax: 81-298-63-2170. E-mail: shimano-tky{at}umin.ac.jp.


Molecular and Cellular Biology, May 2001, p. 2991-3000, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.2991-3000.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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  • Latasa, M.-J., Griffin, M. J., Moon, Y. S., Kang, C., Sul, H. S. (2003). Occupancy and Function of the -150 Sterol Regulatory Element and -65 E-Box in Nutritional Regulation of the Fatty Acid Synthase Gene in Living Animals. Mol. Cell. Biol. 23: 5896-5907 [Abstract] [Full Text]  
  • Le Lay, S., Robichon, C., Le Liepvre, X., Dagher, G., Ferre, P., Dugail, I. (2003). Regulation of ABCA1 expression and cholesterol efflux during adipose differentiation of 3T3-L1 cells. J. Lipid Res. 44: 1499-1507 [Abstract] [Full Text]  
  • Yoshikawa, T., Ide, T., Shimano, H., Yahagi, N., Amemiya-Kudo, M., Matsuzaka, T., Yatoh, S., Kitamine, T., Okazaki, H., Tamura, Y., Sekiya, M., Takahashi, A., Hasty, A. H., Sato, R., Sone, H., Osuga, J.-i., Ishibashi, S., Yamada, N. (2003). Cross-Talk between Peroxisome Proliferator-Activated Receptor (PPAR) {alpha} and Liver X Receptor (LXR) in Nutritional Regulation of Fatty Acid Metabolism. I. PPARs Suppress Sterol Regulatory Element Binding Protein-1c Promoter through Inhibition of LXR Signaling. Mol. Endocrinol. 17: 1240-1254 [Abstract] [Full Text]  
  • Ide, T., Shimano, H., Yoshikawa, T., Yahagi, N., Amemiya-Kudo, M., Matsuzaka, T., Nakakuki, M., Yatoh, S., Iizuka, Y., Tomita, S., Ohashi, K., Takahashi, A., Sone, H., Gotoda, T., Osuga, J.-i., Ishibashi, S., Yamada, N. (2003). Cross-Talk between Peroxisome Proliferator-Activated Receptor (PPAR) {alpha} and Liver X Receptor (LXR) in Nutritional Regulation of Fatty Acid Metabolism. II. LXRs Suppress Lipid Degradation Gene Promoters through Inhibition of PPAR Signaling. Mol. Endocrinol. 17: 1255-1267 [Abstract] [Full Text]  
  • Tontonoz, P., Mangelsdorf, D. J. (2003). Liver X Receptor Signaling Pathways in Cardiovascular Disease. Mol. Endocrinol. 17: 985-993 [Abstract] [Full Text]  
  • Hu, X., Li, S., Wu, J., Xia, C., Lala, D. S. (2003). Liver X Receptors Interact with Corepressors to Regulate Gene Expression. Mol. Endocrinol. 17: 1019-1026 [Abstract] [Full Text]  
  • Kim, S.-W., Park, K., Kwak, E., Choi, E., Lee, S., Ham, J., Kang, H., Kim, J. M., Hwang, S. Y., Kong, Y.-Y., Lee, K., Lee, J. W. (2003). Activating Signal Cointegrator 2 Required for Liver Lipid Metabolism Mediated by Liver X Receptors in Mice. Mol. Cell. Biol. 23: 3583-3592 [Abstract] [Full Text]  
  • Anderson, L. M., Choe, S. E., Yukhananov, R. Y., Hopfner, R. L., Church, G. M., Pratt, R. E., Dzau, V. J. (2003). Identification of a Novel Set of Genes Regulated by a Unique Liver X Receptor-alpha -mediated Transcription Mechanism. J. Biol. Chem. 278: 15252-15260 [Abstract] [Full Text]  
  • Laffitte, B. A., Joseph, S. B., Chen, M., Castrillo, A., Repa, J., Wilpitz, D., Mangelsdorf, D., Tontonoz, P. (2003). The Phospholipid Transfer Protein Gene Is a Liver X Receptor Target Expressed by Macrophages in Atherosclerotic Lesions. Mol. Cell. Biol. 23: 2182-2191 [Abstract] [Full Text]  
  • Juvet, L. K., Andresen, S. M., Schuster, G. U., Dalen, K. T., Tobin, K. A. R., Hollung, K., Haugen, F., Jacinto, S., Ulven, S. M., Bamberg, K., Gustafsson, J.-A., Nebb, H. I. (2003). On the Role of Liver X Receptors in Lipid Accumulation in Adipocytes. Mol. Endocrinol. 17: 172-182 [Abstract] [Full Text]  
  • Thomas, J., Bramlett, K. S., Montrose, C., Foxworthy, P., Eacho, P. I., McCann, D., Cao, G., Kiefer, A., McCowan, J., Yu, K.-l., Grese, T., Chin, W. W., Burris, T. P., Michael, L. F. (2003). A Chemical Switch Regulates Fibrate Specificity for Peroxisome Proliferator-activated Receptor alpha (PPARalpha ) Versus Liver X Receptor. J. Biol. Chem. 278: 2403-2410 [Abstract] [Full Text]  
  • Kocarek, T. A., Dahn, M. S., Cai, H., Strom, S. C., Mercer-Haines, N. A. (2002). Regulation of CYP2B6 and CYP3A Expression by Hydroxymethylglutaryl Coenzyme A Inhibitors in Primary Cultured Human Hepatocytes. Drug Metab. Dispos. 30: 1400-1405 [Abstract] [Full Text]  
  • Muscat, G. E. O., Wagner, B. L., Hou, J., Tangirala, R. K., Bischoff, E. D., Rohde, P., Petrowski, M., Li, J., Shao, G., Macondray, G., Schulman, I. G. (2002). Regulation of Cholesterol Homeostasis and Lipid Metabolism in Skeletal Muscle by Liver X Receptors. J. Biol. Chem. 277: 40722-40728 [Abstract] [Full Text]  
  • Brown, B. G., Cheung, M. C., Lee, A. C., Zhao, X.-Q., Chait, A. (2002). Antioxidant Vitamins and Lipid Therapy: End of a Long Romance?. Arterioscler. Thromb. Vasc. Bio. 22: 1535-1546 [Abstract] [Full Text]  
  • Grefhorst, A., Elzinga, B. M., Voshol, P. J., Plosch, T., Kok, T., Bloks, V. W., van der Sluijs, F. H., Havekes, L. M., Romijn, J. A., Verkade, H. J., Kuipers, F. (2002). Stimulation of Lipogenesis by Pharmacological Activation of the Liver X Receptor Leads to Production of Large, Triglyceride-rich Very Low Density Lipoprotein Particles. J. Biol. Chem. 277: 34182-34190 [Abstract] [Full Text]  
  • Duanmu, Z., Locke, D., Smigelski, J., Wu, W., Dahn, M. S., Falany, C. N., Kocarek, T. A., Runge-Morris, M. (2002). Effects of Dexamethasone on Aryl (SULT1A1)- and Hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes. Drug Metab. Dispos. 30: 997-1004 [Abstract] [Full Text]  
  • Brendel, C., Schoonjans, K., Botrugno, O. A., Treuter, E., Auwerx, J. (2002). The Small Heterodimer Partner Interacts with the Liver X Receptor {alpha} and Represses Its Transcriptional Activity. Mol. Endocrinol. 16: 2065-2076 [Abstract] [Full Text]  
  • Amemiya-Kudo, M., Shimano, H., Hasty, A. H., Yahagi, N., Yoshikawa, T., Matsuzaka, T., Okazaki, H., Tamura, Y., Iizuka, Y., Ohashi, K., Osuga, J.-i., Harada, K., Gotoda, T., Sato, R., Kimura, S., Ishibashi, S., Yamada, N. (2002). Transcriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterogenic genes. J. Lipid Res. 43: 1220-1235 [Abstract] [Full Text]  
  • Menke, J. G., Macnaul, K. L., Hayes, N. S., Baffic, J., Chao, Y.-S., Elbrecht, A., Kelly, L. J., Lam, M.-H., Schmidt, A., Sahoo, S., Wang, J., Wright, S. D., Xin, P., Zhou, G., Moller, D. E., Sparrow, C. P. (2002). A Novel Liver X Receptor Agonist Establishes Species Differences in the Regulation of Cholesterol 7{alpha}-Hydroxylase (CYP7a). Endocrinology 143: 2548-2558 [Abstract] [Full Text]  
  • Matsuzaka, T., Shimano, H., Yahagi, N., Yoshikawa, T., Amemiya-Kudo, M., Hasty, A. H., Okazaki, H., Tamura, Y., Iizuka, Y., Ohashi, K., Osuga, J.-i., Takahashi, A., Yato, S., Sone, H., Ishibashi, S., Yamada, N. (2002). Cloning and characterization of a mammalian fatty acyl-CoA elongase as a lipogenic enzyme regulated by SREBPs. J. Lipid Res. 43: 911-920 [Abstract] [Full Text]  
  • Joseph, S. B., McKilligin, E., Pei, L., Watson, M. A., Collins, A. R., Laffitte, B. A., Chen, M., Noh, G., Goodman, J., Hagger, G. N., Tran, J., Tippin, T. K., Wang, X., Lusis, A. J., Hsueh, W. A., Law, R. E., Collins, J. L., Willson, T. M., Tontonoz, P. (2002). Synthetic LXR ligand inhibits the development of atherosclerosis in mice. Proc. Natl. Acad. Sci. USA 99: 7604-7609 [Abstract] [Full Text]  
  • Repa, J. J., Berge, K. E., Pomajzl, C., Richardson, J. A., Hobbs, H., Mangelsdorf, D. J. (2002). Regulation of ATP-binding Cassette Sterol Transporters ABCG5 and ABCG8 by the Liver X Receptors alpha and beta. J. Biol. Chem. 277: 18793-18800 [Abstract] [Full Text]  
  • Zhang, Y., Mangelsdorf, D. J. (2002). LuXuRies of Lipid Homeostasis: The Unity of Nuclear Hormone Receptors, Transcription Regulation, and Cholesterol Sensing. Mol. Interv. 2: 78-87 [Abstract] [Full Text]  
  • Joseph, S. B., Laffitte, B. A., Patel, P. H., Watson, M. A., Matsukuma, K. E., Walczak, R., Collins, J. L., Osborne, T. F., Tontonoz, P. (2002). Direct and Indirect Mechanisms for Regulation of Fatty Acid Synthase Gene Expression by Liver X Receptors. J. Biol. Chem. 277: 11019-11025 [Abstract] [Full Text]  
  • Tobin, K. A. R., Ulven, S. M., Schuster, G. U., Steineger, H. H., Andresen, S. M., Gustafsson, J.-A., Nebb, H. I. (2002). Liver X Receptors as Insulin-mediating Factors in Fatty Acid and Cholesterol Biosynthesis. J. Biol. Chem. 277: 10691-10697 [Abstract] [Full Text]  
  • del Castillo-Olivares, A., Gil, G. (2002). Differential Effects of Sterol Regulatory Binding Proteins 1 and 2 on Sterol 12alpha -Hydroxylase. SREBP-2 SUPPRESSES THE STEROL 12alpha -HYDROXYLASE PROMOTER. J. Biol. Chem. 277: 6750-6757 [Abstract] [Full Text]  
  • Yang, X.-P., Freeman, L. A., Knapper, C. L., Amar, M. J. A., Remaley, A., Brewer, H. B. Jr., Santamarina-Fojo, S. (2002). The E-box motif in the proximal ABCA1 promoter mediates transcriptional repression of the ABCA1 gene. J. Lipid Res. 43: 297-306 [Abstract] [Full Text]  
  • Edwards, P. A., Kast, H. R., Anisfeld, A. M. (2002). BAREing it all: the adoption of LXR and FXR and their roles in lipid homeostasis. J. Lipid Res. 43: 2-12 [Abstract] [Full Text]  
  • Matsuzaka, T., Shimano, H., Yahagi, N., Amemiya-Kudo, M., Yoshikawa, T., Hasty, A. H., Tamura, Y., Osuga, J.-i., Okazaki, H., Iizuka, Y., Takahashi, A., Sone, H., Gotoda, T., Ishibashi, S., Yamada, N. (2002). Dual regulation of mouse {Delta}5- and {Delta}6-desaturase gene expression by SREBP-1 and PPAR{alpha}. J. Lipid Res. 43: 107-114 [Abstract] [Full Text]  
  • Fu, X., Menke, J. G., Chen, Y., Zhou, G., MacNaul, K. L., Wright, S. D., Sparrow, C. P., Lund, E. G. (2001). 27-Hydroxycholesterol Is an Endogenous Ligand for Liver X Receptor in Cholesterol-loaded Cells. J. Biol. Chem. 276: 38378-38387 [Abstract] [Full Text]  
  • Yoshikawa, T., Shimano, H., Yahagi, N., Ide, T., Amemiya-Kudo, M., Matsuzaka, T., Nakakuki, M., Tomita, S., Okazaki, H., Tamura, Y., Iizuka, Y., Ohashi, K., Takahashi, A., Sone, H., Osuga, J.-i., Gotoda, T., Ishibashi, S., Yamada, N. (2002). Polyunsaturated Fatty Acids Suppress Sterol Regulatory Element-binding Protein 1c Promoter Activity by Inhibition of Liver X Receptor (LXR) Binding to LXR Response Elements. J. Biol. Chem. 277: 1705-1711 [Abstract] [Full Text]