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Molecular and Cellular Biology, May 2001, p. 3001-3011, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.3001-3011.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Two Distinct Domains within CIITA Mediate Self-Association: Involvement of the GTP-Binding and Leucine-Rich Repeat Domains

Michael W. Linhoff, Jonathan A. Harton, Drew E. Cressman, Brian K. Martin,dagger and Jenny Pan-Yun Ting*

Lineberger Comprehensive Cancer Center, Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599-7295

Received 12 December 2000/Returned for modification 19 January 2001/Accepted 6 February 2001

CIITA is the master regulator of class II major histocompatibility complex gene expression. We present evidence that CIITA can self-associate via two domains: the C terminus (amino acids 700 to 1130) and the GTP-binding domain (amino acids 336 to 702). Heterotypic and homotypic interactions are observed between these two regions. Deletions within the GTP-binding domain that reduce GTP-binding and transactivation function also reduce self-association. In addition, two leucine residues in the C-terminal leucine-rich repeat region are critical for self-association as well as function. This study reveals for the first time a complex pattern of CIITA self-association. These interactions are discussed with regard to the apoptosis signaling proteins, Apaf-1 and Nod1, which share domain arrangements similar to those of CIITA.

* Corresponding author. Mailing address: Lineberger Comprehensive Cancer Center, Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295. Phone: (919) 966-5538. Fax: (919) 966-8212. E-mail: panyun{at}med.unc.edu.

dagger Present address: Department of Microbiology, University of Iowa, Iowa City, IA 52242.

Molecular and Cellular Biology, May 2001, p. 3001-3011, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.3001-3011.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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