Role of NF-Y in In Vivo Regulation of the {gamma}-Globin Gene

doi:10.1128/MCB.21.9.3083-3095.2001

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Duan, Z.
	Articles by Li, Q.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Duan, Z.
	Articles by Li, Q.

Previous Article | Next Article

Molecular and Cellular Biology, May 2001, p. 3083-3095, Vol. 21, No. 9
0270-7306/01/$04.00+0 DOI: 10.1128/MCB.21.9.3083-3095.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of NF-Y in In Vivo Regulation of the $gamma$ -Globin Gene

Zhijun Duan, George Stamatoyannopoulos, and Qiliang Li^*

Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington 98195

Received 13 September 2000/Returned for modification 25 October 2000/Accepted 9 February 2001

The duplicated CCAAT box is required for $gamma$ gene expression. We report here that the transcriptional factor NF-Y is recruitedto the duplicated CCAAT box in vivo. A mutation of the duplicatedCCAAT box that severely disrupts the NF-Y binding also reducesthe accessibility level of the $gamma$ gene promoter, affects the assemblyof basal transcriptional machinery, and increases the recruitmentof GATA-1 to the locus control region (LCR) and the proximal promoterand the recruitment of transcription cofactor CBP/p300 to theLCR. These findings suggest that recruitment of NF-Y to the duplicatedCCAAT box plays a role in the chromatin opening of the $gamma$ genepromoter as well as in the communication between the $gamma$ gene promoterand theLCR.

^* Corresponding author. Mailing address: Medical Genetics, Box 357720, University of Washington, Seattle, WA 98195. Phone: (206)616-4526.Fax: (206)543-3050. E-mail: LI111640{at}u.washington.edu.

This article has been cited by other articles:

Yin, W., Barkess, G., Fang, X., Xiang, P., Cao, H., Stamatoyannopoulos, G., Li, Q. (2007). Histone acetylation at the human {beta}-globin locus changes with developmental age. Blood 110: 4101-4107 [Abstract] [Full Text]
Olave, I. A., Doneanu, C., Fang, X., Stamatoyannopoulos, G., Li, Q. (2007). Purification and Identification of Proteins That Bind to the Hereditary Persistence of Fetal Hemoglobin -198 Mutation in the {gamma}-Globin Gene Promoter. J. Biol. Chem. 282: 853-862 [Abstract] [Full Text]
Crusselle-Davis, V. J., Vieira, K. F., Zhou, Z., Anantharaman, A., Bungert, J. (2006). Antagonistic Regulation of {beta}-Globin Gene Expression by Helix-Loop-Helix Proteins USF and TFII-I.. Mol. Cell. Biol. 26: 6832-6843 [Abstract] [Full Text]
Donati, G., Imbriano, C., Mantovani, R. (2006). Dynamic recruitment of transcription factors and epigenetic changes on the ER stress response gene promoters. Nucleic Acids Res 34: 3116-3127 [Abstract] [Full Text]
Su, M., Lee, D., Ganss, B., Sodek, J. (2006). Stereochemical Analysis of the Functional Significance of the Conserved Inverted CCAAT and TATA Elements in the Rat Bone Sialoprotein Gene Promoter. J. Biol. Chem. 281: 9882-9890 [Abstract] [Full Text]
Bultman, S. J., Gebuhr, T. C., Magnuson, T. (2005). A Brg1 mutation that uncouples ATPase activity from chromatin remodeling reveals an essential role for SWI/SNF-related complexes in {beta}-globin expression and erythroid development. Genes Dev. 19: 2849-2861 [Abstract] [Full Text]
Yu, X., Zhu, X., Pi, W., Ling, J., Ko, L., Takeda, Y., Tuan, D. (2005). The Long Terminal Repeat (LTR) of ERV-9 Human Endogenous Retrovirus Binds to NF-Y in the Assembly of an Active LTR Enhancer Complex NF-Y/MZF1/GATA-2. J. Biol. Chem. 280: 35184-35194 [Abstract] [Full Text]
Kabe, Y., Yamada, J., Uga, H., Yamaguchi, Y., Wada, T., Handa, H. (2005). NF-Y Is Essential for the Recruitment of RNA Polymerase II and Inducible Transcription of Several CCAAT Box-Containing Genes. Mol. Cell. Biol. 25: 512-522 [Abstract] [Full Text]
Vilaboa, N., Bermejo, R., Martinez, P., Bornstein, R., Cales, C. (2004). A novel E2 box-GATA element modulates Cdc6 transcription during human cells polyploidization. Nucleic Acids Res 32: 6454-6467 [Abstract] [Full Text]
Addya, S., Keller, M. A., Delgrosso, K., Ponte, C. M., Vadigepalli, R., Gonye, G. E., Surrey, S. (2004). Erythroid-induced commitment of K562 cells results in clusters of differentially expressed genes enriched for specific transcription regulatory elements. Physiol. Genomics 19: 117-130 [Abstract] [Full Text]
Huang, D.-Y., Kuo, Y.-Y., Lai, J.-S., Suzuki, Y., Sugano, S., Chang, Z.-F. (2004). GATA-1 and NF-Y cooperate to mediate erythroid-specific transcription of Gfi-1B gene. Nucleic Acids Res 32: 3935-3946 [Abstract] [Full Text]
Zhu, Q.-S., Qian, B., Levy, D. (2004). CCAAT/Enhancer-binding Protein {alpha} (C/EBP{alpha}) Activates Transcription of the Human Microsomal Epoxide Hydrolase Gene (EPHX1) through the Interaction with DNA-bound NF-Y. J. Biol. Chem. 279: 29902-29910 [Abstract] [Full Text]
Li, Q., Fang, X., Han, H., Stamatoyannopoulos, G. (2004). The minimal promoter plays a major role in silencing of the galago {gamma}-globin gene in adult erythropoiesis. Proc. Natl. Acad. Sci. USA 101: 8096-8101 [Abstract] [Full Text]
Fang, X., Han, H., Stamatoyannopoulos, G., Li, Q. (2004). Developmentally Specific Role of the CCAAT Box in Regulation of Human {gamma}-Globin Gene Expression. J. Biol. Chem. 279: 5444-5449 [Abstract] [Full Text]
Pi, W., Yang, Z., Wang, J., Ruan, L., Yu, X., Ling, J., Krantz, S., Isales, C., Conway, S. J., Lin, S., Tuan, D. (2004). The LTR enhancer of ERV-9 human endogenous retrovirus is active in oocytes and progenitor cells in transgenic zebrafish and humans. Proc. Natl. Acad. Sci. USA 101: 805-810 [Abstract] [Full Text]
Letting, D. L., Chen, Y.-Y., Rakowski, C., Reedy, S., Blobel, G. A. (2004). Context-dependent regulation of GATA-1 by friend of GATA-1. Proc. Natl. Acad. Sci. USA 101: 476-481 [Abstract] [Full Text]
Duan, Z., Horwitz, M. (2003). Targets of the transcriptional repressor oncoprotein Gfi-1. Proc. Natl. Acad. Sci. USA 100: 5932-5937 [Abstract] [Full Text]
Nowling, T., Bernadt, C., Johnson, L., Desler, M., Rizzino, A. (2003). The Co-activator p300 Associates Physically with and Can Mediate the Action of the Distal Enhancer of the FGF-4 Gene. J. Biol. Chem. 278: 13696-13705 [Abstract] [Full Text]
Johnson, K. D., Grass, J. A., Boyer, M. E., Kiekhaefer, C. M., Blobel, G. A., Weiss, M. J., Bresnick, E. H. (2002). Cooperative activities of hematopoietic regulators recruit RNA polymerase II to a tissue-specific chromatin domain. Proc. Natl. Acad. Sci. USA 99: 11760-11765 [Abstract] [Full Text]
Duan, Z.-J., Fang, X., Rohde, A., Han, H., Stamatoyannopoulos, G., Li, Q. (2002). Developmental specificity of recruitment of TBP to the TATA box of the human gamma -globin gene. Proc. Natl. Acad. Sci. USA 99: 5509-5514 [Abstract] [Full Text]
Horak, C. E., Mahajan, M. C., Luscombe, N. M., Gerstein, M., Weissman, S. M., Snyder, M. (2002). GATA-1 binding sites mapped in the beta -globin locus by using mammalian chIp-chip analysis. Proc. Natl. Acad. Sci. USA 10.1073/pnas.052706999v1 [Abstract] [Full Text]
Frontini, M., Imbriano, C., diSilvio, A., Bell, B., Bogni, A., Romier, C., Moras, D., Tora, L., Davidson, I., Mantovani, R. (2002). NF-Y Recruitment of TFIID, Multiple Interactions with Histone Fold TAFIIs. J. Biol. Chem. 277: 5841-5848 [Abstract] [Full Text]
Horak, C. E., Mahajan, M. C., Luscombe, N. M., Gerstein, M., Weissman, S. M., Snyder, M. (2002). GATA-1 binding sites mapped in the beta -globin locus by using mammalian chIp-chip analysis. Proc. Natl. Acad. Sci. USA 99: 2924-2929 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals

Role of NF-Y in In Vivo Regulation of the -Globin Gene

Role of NF-Y in In Vivo Regulation of the $gamma$ -Globin Gene