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Molecular and Cellular Biology, May 2001, p. 3159-3165, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.3159-3165.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Generation and Analysis of Mice Lacking the Chemokine Fractalkine

Donald N. Cook,dagger Shu-Cheng Chen, Lee M. Sullivan, Denise J. Manfra, Maria T. Wiekowski, Dina M. Prosser, Galya Vassileva, and Sergio A. Lira*

Department of Immunology, Schering-Plough Research Institute, Kenilworth, New Jersey 07033

Received 25 October 2000/Returned for modification 8 January 2001/Accepted 29 January 2001

Fractalkine (CX3CL1) is the first described chemokine that can exist either as a soluble protein or as a membrane-bound molecule. Both forms of fractalkine can mediate adhesion of cells expressing its receptor, CX3CR1. This activity, together with its expression on endothelial cells, suggests that fractalkine might mediate adhesion of leukocytes to the endothelium during inflammation. Fractalkine is also highly expressed in neurons, and its receptor, CX3CR1, is expressed on glial cells. To determine the biologic role of fractalkine, we used targeted gene disruption to generate fractalkine-deficient mice. These mice did not exhibit overt behavioral abnormalities, and histologic analysis of their brains did not reveal any gross changes compared to wild-type mice. In addition, these mice had normal hematologic profiles except for a decrease in the number of blood leukocytes expressing the cell surface marker F4/80. The cellular composition of their lymph nodes did not differ significantly from that of wild-type mice. Similarly, the responses of fractalkine-/- mice to a variety of inflammatory stimuli were indistinguishable from those of wild-type mice.

* Corresponding author. Mailing address: Department of Immunology, Schering-Plough Research Institute, 2015 Galloping Hill Rd., Kenilworth, NJ 07033. Phone: (908) 740-3088. Fax: (908) 740-3084. E-mail: sergio.lira{at}spcorp.com.

dagger Present address: Division of Pulmonary and Critical Care Medicine, Duke University Medical Center, Durham, NC 27710.

Molecular and Cellular Biology, May 2001, p. 3159-3165, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.3159-3165.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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