This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ekholm, S. V.
Right arrow Articles by Zetterberg, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ekholm, S. V.
Right arrow Articles by Zetterberg, A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, May 2001, p. 3256-3265, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.3256-3265.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Accumulation of Cyclin E Is Not a Prerequisite for Passage through the Restriction Point

Susanna V. Ekholm,1,2 Peter Zickert,1 Steven I. Reed,2 and Anders Zetterberg1,*

Department of Oncology-Pathology, Cellular and Molecular Tumorpathology, Karolinska Institute/Karolinska Hospital, S-171 76 Stockholm, Sweden,1 and Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 920372

Received 18 October 2000/Returned for modification 27 November 2000/Accepted 9 February 2001

The restriction point (R) is defined as the point in G1 after which cells can complete a division cycle without growth factors and divides G1 into two physiologically different intervals in cycling cells, G1-pm (a postmitotic interval with a constant length of 3 to 4 h) and G1-ps (a pre-DNA-synthetic interval with a variable length of 1 to 10 h). Cyclin E is a G1 regulatory protein whose accumulation has been suggested to be critical for passage through R. We have studied cyclin E protein levels in individual cells of asynchronously growing cell populations, with respect to both passage through R and entry into S phase. We found that the postmitotic G1 cells that had not yet reached R were negative for cyclin E accumulation. On the other hand, cells that had passed R were found to accumulate cyclin E at variable times (1 to 8 h) after passage through R and 2 to 5 h before entry into S. These kinetic data rule out the hypothesis that passage through R is dependent on the accumulation of cyclin E but suggest, instead, the converse, that passage through R is a prerequisite for cyclin E accumulation. Furthermore, we found that most of the cyclin E protein is downregulated within 1 to 2 h after entry into S.

* Corresponding author. Mailing address: Department of Oncology and Pathology, Karolinska Institute, Karolinska Sjukhuset, CCK R8:04, 171 76 Stockholm, Sweden. Phone: (46) 851-77-5250. Fax: (46) 832-1047. E-mail: anders.zetterberg{at}onkpat.ki.se.

Molecular and Cellular Biology, May 2001, p. 3256-3265, Vol. 21, No. 9
0270-7306/01/$04.00+0   DOI: 10.1128/MCB.21.9.3256-3265.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chartier, N. T., Oddou, C. I., Laine, M. G., Ducarouge, B., Marie, C. A., Block, M. R., Jacquier-Sarlin, M. R. (2007). Cyclin-Dependent Kinase 2/Cyclin E Complex Is Involved in p120 Catenin (p120ctn) Dependent Cell Growth Control: A New Role for p120ctn in Cancer. Cancer Res. 67: 9781-9790 [Abstract] [Full Text]  
  • Matsumoto, Y., Maller, J. L. (2004). A Centrosomal Localization Signal in Cyclin E Required for Cdk2-Independent S Phase Entry. Science 306: 885-888 [Abstract] [Full Text]  
  • Kawamura, K., Izumi, H., Ma, Z., Ikeda, R., Moriyama, M., Tanaka, T., Nojima, T., Levin, L. S., Fujikawa-Yamamoto, K., Suzuki, K., Fukasawa, K. (2004). Induction of Centrosome Amplification and Chromosome Instability in Human Bladder Cancer Cells by p53 Mutation and Cyclin E Overexpression. Cancer Res. 64: 4800-4809 [Abstract] [Full Text]  
  • Ekholm-Reed, S., Mendez, J., Tedesco, D., Zetterberg, A., Stillman, B., Reed, S. I. (2004). Deregulation of cyclin E in human cells interferes with prereplication complex assembly. JCB 165: 789-800 [Abstract] [Full Text]  
  • Gad, A., Thullberg, M., Dannenberg, J.-H., Riele, H. t., Stromblad, S. (2004). Retinoblastoma Susceptibility Gene Product (pRb) and p107 Functionally Separate the Requirements for Serum and Anchorage in the Cell Cycle G1-phase. J. Biol. Chem. 279: 13640-13644 [Abstract] [Full Text]  
  • Reed, S. E., Spruck, C. H., Sangfelt, O., van Drogen, F., Mueller-Holzner, E., Widschwendter, M., Zetterberg, A., Reed, S. I. (2004). Mutation of hCDC4 Leads to Cell Cycle Deregulation of Cyclin E in Cancer. Cancer Res. 64: 795-800 [Abstract] [Full Text]  
  • Paternot, S., Coulonval, K., Dumont, J. E., Roger, P. P. (2003). Cyclic AMP-dependent Phosphorylation of Cyclin D3-bound CDK4 Determines the Passage through the Cell Cycle Restriction Point in Thyroid Epithelial Cells. J. Biol. Chem. 278: 26533-26540 [Abstract] [Full Text]  
  • Schorl, C., Sedivy, J. M. (2003). Loss of Protooncogene c-Myc Function Impedes G1 Phase Progression Both before and after the Restriction Point. Mol. Biol. Cell 14: 823-835 [Abstract] [Full Text]  
  • Wiebusch, L., Asmar, J., Uecker, R., Hagemeier, C. (2003). Human cytomegalovirus immediate-early over protein 2 (IE2)-mediated activation of cyclin E is cell-cycle-independent and forces S-phase entry in IE2-arrested cells. J. Gen. Virol. 84: 51-60 [Abstract] [Full Text]  
  • Feliers, D., Frank, M. A., Riley, D. J. (2002). Activation of Cyclin D1-Cdk4 and Cdk4-Directed Phosphorylation of RB Protein in Diabetic Mesangial Hypertrophy. Diabetes 51: 3290-3299 [Abstract] [Full Text]  
  • Spruck, C. H., Strohmaier, H., Sangfelt, O., Muller, H. M., Hubalek, M., Muller-Holzner, E., Marth, C., Widschwendter, M., Reed, S. I. (2002). hCDC4 Gene Mutations in Endometrial Cancer. Cancer Res. 62: 4535-4539 [Abstract] [Full Text]  
  • Farkas, T., Hansen, K., Holm, K., Lukas, J., Bartek, J. (2002). Distinct Phosphorylation Events Regulate p130- and p107-mediated Repression of E2F-4. J. Biol. Chem. 277: 26741-26752 [Abstract] [Full Text]  
  • Mazumder, S., Gong, B., Chen, Q., Drazba, J. A., Buchsbaum, J. C., Almasan, A. (2002). Proteolytic Cleavage of Cyclin E Leads to Inactivation of Associated Kinase Activity and Amplification of Apoptosis in Hematopoietic Cells. Mol. Cell. Biol. 22: 2398-2409 [Abstract] [Full Text]  
  • Glassford, J., Holman, M., Banerji, L., Clayton, E., Klaus, G. G. B., Turner, M., Lam, E. W.-F. (2001). Vav Is Required for Cyclin D2 Induction and Proliferation of Mouse B Lymphocytes Activated via the Antigen Receptor. J. Biol. Chem. 276: 41040-41048 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»