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Molecular and Cellular Biology, May 2002, p. 3276-3280, Vol. 22, No. 10
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.10.3276-3280.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Testicular Germ Cell-Associated Serine-Threonine Kinase, MAK, Is Dispensable for Sperm Formation

Yoichi Shinkai,1* Hideo Satoh,2,{dagger} Naoki Takeda,2,{ddagger} Mikiko Fukuda,1 Emiko Chiba,2 Tomoya Kato,3 Takashi Kuramochi,4,§ and Yoshihiko Araki3

Institute for Virus Research, Kyoto University, Kyoto 606-8507,1 Department of Oncology, Nippon Roche Research Center, Kamakura 247-0063,2 Department of Immunology and Parasitology, Yamagata University School of Medicine, Yamagata 990-9585,3 Central Institute for Experimental Animals, Kawasaki 216-0001, Japan4

Received 10 December 2001/ Returned for modification 14 January 2002/ Accepted 4 February 2002

A member of the mitogen-activated protein kinase superfamily, MAK, has been proposed to have an important role in spermatogenesis, since Mak gene expression is highly restricted to testicular germ cells. To assess the biological function of MAK, we have established MAK-deficient (Mak-/-) mice. Mak-/- mice developed normally, and no gross abnormalities were observed. Spermatogenesis of the Mak-/- mice was also intact, and most of the mice were fertile. However, Mak-/- male-derived litter sizes and their sperm motility in vitro were mildly reduced. These data show that function of MAK is not essential for spermatogenesis and male fertility.

* Corresponding author. Mailing address: Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin, Kawara-cho, Kyoto 606-8507, Japan. Phone: 81-75-751-3990. Fax: 81-75-751-3991. E-mail: yshinkai{at}virus.kyoto-u.ac.jp.

{dagger} Present address: Environmental Health Science Laboratory, Sumitomo Chemical Co. Ltd., Osaka 554-8558, Japan.

{ddagger} Present address: Center for Animal Resources and Development (CARD), Kumamoto University, Kumamoto 860-0811, Japan.

§ Present address: Mitsubishi Kagaku Institute of Life Sciences, Machida 194-8511, Japan.

Molecular and Cellular Biology, May 2002, p. 3276-3280, Vol. 22, No. 10
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.10.3276-3280.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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