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Molecular and Cellular Biology, June 2002, p. 3769-3782, Vol. 22, No. 11
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.11.3769-3782.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Paip1 Interacts with Poly(A) Binding Protein through Two Independent Binding Motifs

Guylaine Roy,1 Gregory De Crescenzo,2 Kianoush Khaleghpour,1,2 Avak Kahvejian,1 Maureen O'Connor-McCourt,2 and Nahum Sonenberg1*

Department of Biochemistry and McGill Cancer Centre, McGill University, Montréal, Québec, Canada H3G 1Y6,1 The Biotechnology Research Institute, National Research Council of Canada, Montréal, Québec, Canada H4P 2R22

Received 20 December 2001/ Returned for modification 7 February 2002/ Accepted 25 February 2002

The 3' poly(A) tail of eukaryotic mRNAs plays an important role in the regulation of translation. The poly(A) binding protein (PABP) interacts with eukaryotic initiation factor 4G (eIF4G), a component of the eIF4F complex, which binds to the 5' cap structure. The PABP-eIF4G interaction brings about the circularization of the mRNA by joining its 5' and 3' termini, thereby stimulating mRNA translation. The activity of PABP is regulated by two interacting proteins, Paip1 and Paip2. To study the mechanism of the Paip1-PABP interaction, far-Western, glutathione S-transferase pull-down, and surface plasmon resonance experiments were performed. Paip1 contains two binding sites for PABP, PAM1 and PAM2 (for PABP-interacting motifs 1 and 2). PAM2 consists of a 15-amino-acid stretch residing in the N terminus, and PAM1 encompasses a larger C-terminal acidic-amino-acid-rich region. PABP also contains two Paip1 binding sites, one located in RNA recognition motifs 1 and 2 and the other located in the C-terminal domain. Paip1 binds to PABP with a 1:1 stoichiometry and an apparent Kd of 1.9 nM.

* Corresponding author. Mailing address: Department of Biochemistry and McGill Cancer Centre, McGill University, 3655 Promenade Sir William Osler, Montréal, Québec, Canada H3G 1Y6. Phone: (514) 398-7274. Fax: (514) 398-1287. E-mail: nahum.sonenberg{at}mcgill.ca.

Molecular and Cellular Biology, June 2002, p. 3769-3782, Vol. 22, No. 11
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.11.3769-3782.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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