This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ricci, A. R.
Right arrow Articles by Brandl, C. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ricci, A. R.
Right arrow Articles by Brandl, C. J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2002, p. 4033-4042, Vol. 22, No. 12
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.12.4033-4042.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Components of the SAGA Histone Acetyltransferase Complex Are Required for Repressed Transcription of ARG1 in Rich Medium

Andrea R. Ricci, Julie Genereaux, and Christopher J. Brandl*

Department of Biochemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada

Received 29 January 2002/ Returned for modification 11 March 2002/ Accepted 20 March 2002

Transcriptional regulation of the Saccharomyces cerevisiae ARG1 gene is controlled by positive and negative elements. The transactivator Gcn4p is required for activation in minimal medium, while arginine repression requires the ArgR/Mcm1 regulatory complex, which binds to two upstream arginine control elements. We have found that the coordinated regulation of ARG1 requires components of the SAGA chromatin-remodeling complex. Using gcn5 deletion strains and a Gcn5 protein carrying the E173Q mutation in the histone acetyltransferase (HAT) region, we show that the HAT activity of Gcn5p is required for repression of ARG1 in rich medium. Similar increases in expression were seen upon deletion of other SAGA components but not upon deletion of the ADA-specific component, Ahc1p. Chromatin immunoprecipitations using antibodies to acetylated H3 confirmed that a decrease in the level of acetylated histones at the ARG1 promoter correlated with increased ARG1 expression. Up-regulation of ARG1 in the absence of Gcn5p also correlated with increased binding of TATA-binding protein to the promoter. The analysis of promoter deletions showed that Gcn5/Ada repression of ARG1 was mediated through the action of the ArgR/Mcm1 regulatory complex. In addition, studies with minimal medium demonstrated a requirement for the Ada proteins in activation of ARG1. This suggests that SAGA has a dual role at ARG1, acting to repress transcription in rich medium and activate transcription in minimal medium.

* Corresponding author. Mailing address: Department of Biochemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada. Phone: (519) 661-2111, ext. 86857. Fax: (519) 661-3175. E-mail: cbrandl{at}uwo.ca.

Molecular and Cellular Biology, June 2002, p. 4033-4042, Vol. 22, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.12.4033-4042.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jacobson, S., Pillus, L. (2009). The SAGA Subunit Ada2 Functions in Transcriptional Silencing. Mol. Cell. Biol. 29: 6033-6045 [Abstract] [Full Text]  
  • Helmlinger, D., Marguerat, S., Villen, J., Gygi, S. P., Bahler, J., Winston, F. (2008). The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8. Genes Dev. 22: 3184-3195 [Abstract] [Full Text]  
  • Reyes-Dominguez, Y., Narendja, F., Berger, H., Gallmetzer, A., Fernandez-Martin, R., Garcia, I., Scazzocchio, C., Strauss, J. (2008). Nucleosome Positioning and Histone H3 Acetylation Are Independent Processes in the Aspergillus nidulans prnD-prnB Bidirectional Promoter. Eukaryot Cell 7: 656-663 [Abstract] [Full Text]  
  • Mutiu, A. I., Hoke, S. M. T., Genereaux, J., Hannam, C., MacKenzie, K., Jobin-Robitaille, O., Guzzo, J., Cote, J., Andrews, B., Haniford, D. B., Brandl, C. J. (2007). Structure/Function Analysis of the Phosphatidylinositol-3-Kinase Domain of Yeast Tra1. Genetics 177: 151-166 [Abstract] [Full Text]  
  • Qi, D., Jin, H., Lilja, T., Mannervik, M. (2006). Drosophila Reptin and Other TIP60 Complex Components Promote Generation of Silent Chromatin. Genetics 174: 241-251 [Abstract] [Full Text]  
  • Saddic, L. A., Huvermann, B., Bezhani, S., Su, Y., Winter, C. M., Kwon, C. S., Collum, R. P., Wagner, D. (2006). The LEAFY target LMI1 is a meristem identity regulator and acts together with LEAFY to regulate expression of CAULIFLOWER. Development 133: 1673-1682 [Abstract] [Full Text]  
  • Liu, Y., Xu, X., Singh-Rodriguez, S., Zhao, Y., Kuo, M.-H. (2005). Histone H3 Ser10 Phosphorylation-Independent Function of Snf1 and Reg1 Proteins Rescues a gcn5- Mutant in HIS3 Expression. Mol. Cell. Biol. 25: 10566-10579 [Abstract] [Full Text]  
  • Lee, K. K., Florens, L., Swanson, S. K., Washburn, M. P., Workman, J. L. (2005). The Deubiquitylation Activity of Ubp8 Is Dependent upon Sgf11 and Its Association with the SAGA Complex. Mol. Cell. Biol. 25: 1173-1182 [Abstract] [Full Text]  
  • Powell, D. W., Weaver, C. M., Jennings, J. L., McAfee, K. J., He, Y., Weil, P. A., Link, A. J. (2004). Cluster Analysis of Mass Spectrometry Data Reveals a Novel Component of SAGA. Mol. Cell. Biol. 24: 7249-7259 [Abstract] [Full Text]  
  • Qiu, H., Hu, C., Yoon, S., Natarajan, K., Swanson, M. J., Hinnebusch, A. G. (2004). An Array of Coactivators Is Required for Optimal Recruitment of TATA Binding Protein and RNA Polymerase II by Promoter-Bound Gcn4p. Mol. Cell. Biol. 24: 4104-4117 [Abstract] [Full Text]  
  • Stebbins, J. L., Triezenberg, S. J. (2004). Identification, Mutational Analysis, and Coactivator Requirements of Two Distinct Transcriptional Activation Domains of the Saccharomyces cerevisiae Hap4 Protein. Eukaryot Cell 3: 339-347 [Abstract] [Full Text]  
  • Zwartjes, C. G. M., Jayne, S., van den Berg, D. L. C., Timmers, H. T. M. (2004). Repression of Promoter Activity by CNOT2, a Subunit of the Transcription Regulatory Ccr4-Not Complex. J. Biol. Chem. 279: 10848-10854 [Abstract] [Full Text]  
  • Dziak, R., Leishman, D., Radovic, M., Tye, B. K., Yankulov, K. (2003). Evidence for a Role of MCM (Mini-chromosome Maintenance)5 in Transcriptional Repression of Sub-telomeric and Ty-proximal Genes in Saccharomyces cerevisiae. J. Biol. Chem. 278: 27372-27381 [Abstract] [Full Text]  
  • Bertrand, C., Bergounioux, C., Domenichini, S., Delarue, M., Zhou, D.-X. (2003). Arabidopsis Histone Acetyltransferase AtGCN5 Regulates the Floral Meristem Activity through the WUSCHEL/AGAMOUS Pathway. J. Biol. Chem. 278: 28246-28251 [Abstract] [Full Text]  
  • Swanson, M. J., Qiu, H., Sumibcay, L., Krueger, A., Kim, S.-j., Natarajan, K., Yoon, S., Hinnebusch, A. G. (2003). A Multiplicity of Coactivators Is Required by Gcn4p at Individual Promoters In Vivo. Mol. Cell. Biol. 23: 2800-2820 [Abstract] [Full Text]  
  • Vlachonasios, K. E., Thomashow, M. F., Triezenberg, S. J. (2003). Disruption Mutations of ADA2b and GCN5 Transcriptional Adaptor Genes Dramatically Affect Arabidopsis Growth, Development, and Gene Expression. Plant Cell 15: 626-638 [Abstract] [Full Text]  
  • Turner, S. D., Ricci, A. R., Petropoulos, H., Genereaux, J., Skerjanc, I. S., Brandl, C. J. (2002). The E2 Ubiquitin Conjugase Rad6 Is Required for the ArgR/Mcm1 Repression of ARG1 Transcription. Mol. Cell. Biol. 22: 4011-4019 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»