This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, S.-K. Articles by Prakash, S. Search for Related Content PubMed PubMed Citation Articles by Lee, S.-K. Articles by Prakash, S.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2002, p. 4383-4389, Vol. 22, No. 12
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.12.4383-4389.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Yeast RAD26, a Homolog of the Human CSB Gene, Functions Independently of Nucleotide Excision Repair and Base Excision Repair in Promoting Transcription through Damaged Bases

Sung-Keun Lee,^, Sung-Lim Yu,^, Louise Prakash, and Satya Prakash^*

Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1061

Received 11 January 2002/ Returned for modification 13 February 2002/ Accepted 12 March 2002

RAD26 in the yeast Saccharomyces cerevisiae is the counterpart of the human Cockayne syndrome group B (CSB) gene. Both RAD26 and CSB act in the preferential repair of UV lesions on the transcribed strand, and in this process, they function together with the components of nucleotide excision repair (NER). Here, we examine the role of RAD26 in the repair of DNA lesions induced upon treatment with the alkylating agent methyl methanesulfonate (MMS). MMS-induced DNA lesions include base damages such as 3-methyl adenine and 7-methyl guanine, and these lesions are removed in yeast by the alternate competing pathways of base excision repair (BER), which is initiated by the action of MAG1-encoded N-methyl purine DNA glycosylase, and NER. Interestingly, a synergistic increase in MMS sensitivity was observed in the rad26 strain upon inactivation of NER or BER, indicating that RAD26 promotes the survival of MMS-treated cells by a mechanism that acts independently of either of these repair pathways. The galactose-inducible transcription of the GAL2, GAL7, and GAL10 genes is reduced in MMS-treated rad26 cells and also in mag1 rad14 cells, whereas a very severe reduction in transcription occurs in MMS-treated mag1 rad14 rad26 cells. From these observations, we infer that RAD26 plays a role in promoting transcription by RNA polymerase II through damaged bases. The implications of these observations are discussed in this paper.

Present address: WonKwang University, Iksan, Chonbuk 570-749, South Korea.

This article has been cited by other articles:

• Brueckner, F., Hennecke, U., Carell, T., Cramer, P. (2007). CPD Damage Recognition by Transcribing RNA Polymerase II. Science 315: 859-862 [Abstract] [Full Text]  
• Conconi, A., Paquette, M., Fahy, D., Bespalov, V. A., Smerdon, M. J. (2005). Repair-Independent Chromatin Assembly onto Active Ribosomal Genes in Yeast after UV Irradiation. Mol. Cell. Biol. 25: 9773-9783 [Abstract] [Full Text]  
• Kaplan, C. D., Holland, M. J., Winston, F. (2005). Interaction between Transcription Elongation Factors and mRNA 3'-End Formation at the Saccharomyces cerevisiae GAL10-GAL7 Locus. J. Biol. Chem. 280: 913-922 [Abstract] [Full Text]  
• Horibata, K., Iwamoto, Y., Kuraoka, I., Jaspers, N. G. J., Kurimasa, A., Oshimura, M., Ichihashi, M., Tanaka, K. (2004). From The Cover: Complete absence of Cockayne syndrome group B gene product gives rise to UV-sensitive syndrome but not Cockayne syndrome. Proc. Natl. Acad. Sci. USA 101: 15410-15415 [Abstract] [Full Text]  
• de Waard, H., de Wit, J., Andressoo, J.-O., van Oostrom, C. T. M., Riis, B., Weimann, A., Poulsen, H. E., van Steeg, H., Hoeijmakers, J. H. J., van der Horst, G. T. J. (2004). Different Effects of CSA and CSB Deficiency on Sensitivity to Oxidative DNA Damage. Mol. Cell. Biol. 24: 7941-7948 [Abstract] [Full Text]  
• Laurencon, A., Orme, C. M., Peters, H. K., Boulton, C. L., Vladar, E. K., Langley, S. A., Bakis, E. P., Harris, D. T., Harris, N. J., Wayson, S. M., Hawley, R. S., Burtis, K. C. (2004). A Large-Scale Screen for Mutagen-Sensitive Loci in Drosophila. Genetics 167: 217-231 [Abstract] [Full Text]  
• Svejstrup, J. Q. (2003). Rescue of arrested RNA polymerase II complexes. J. Cell Sci. 116: 447-451 [Abstract] [Full Text]