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Molecular and Cellular Biology, June 2002, p. 4402-4418, Vol. 22, No. 12
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.12.4402-4418.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The G1 Cyclin Cln3 Promotes Cell Cycle Entry via the Transcription Factor Swi6

Herman Wijnen,1,2,{dagger} Allison Landman,1,{ddagger} and Bruce Futcher1,3*

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724,1 Graduate Program in Genetics, State University of New York, Stony Brook, New York 11792,2 Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, New York 11794-52223

Received 19 December 2001/ Returned for modification 15 February 2002/ Accepted 18 March 2002

In Saccharomyces cerevisiae (budding yeast), commitment to cell division in late G1 is promoted by the G1 cyclin Cln3 and its associated cyclin-dependent kinase, Cdc28. We show here that all known aspects of the function of Cln3 in G1 phase, including control of cell size, pheromone sensitivity, cell cycle progress, and transcription, require the protein Swi6. Swi6 is a component of two related transcription factors, SBF and MBF, which are known to regulate many genes at the G1-S transition. The Cln3-Cdc28 complex somehow activates SBF and MBF, but there was no evidence for direct phosphorylation of SBF/MBF by Cln3-Cdc28 or for a stable complex between SBF/MBF and Cln3-Cdc28. The activation also does not depend on the ability of Cln3 to activate transcription when artificially recruited directly to a promoter. The amino terminus and the leucine zipper of Swi6 are important for the ability of Swi6 to respond to Cln3 but are not essential for the basal transcriptional activity of Swi6. Cln3-Cdc28 may activate SBF and MBF indirectly, perhaps by phosphorylating some intermediary protein.

* Corresponding author. Mailing address: Department of Microbiology, State University of New York, Stony Brook, NY 11794-5222. Phone: (631) 632-4715. Fax: (631) 632-9797. E-mail: bfutcher{at}ms.cc.sunysb.edu.

{dagger} Present address: Department of Genetics, The Rockefeller University, New York, NY 10021.

{ddagger} Present address: Brandeis University, Waltham, MA 02454.

Molecular and Cellular Biology, June 2002, p. 4402-4418, Vol. 22, No. 12
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.12.4402-4418.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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