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Molecular and Cellular Biology, July 2002, p. 4512-4521, Vol. 22, No. 13
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.13.4512-4521.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Recombinational Telomere Elongation Promoted by DNA Circles

Shobhana Natarajan and Michael J. McEachern*

Department of Genetics, University of Georgia, Athens, Georgia 30602-7223

Received 23 October 2001/ Returned for modification 17 December 2001/ Accepted 1 April 2002

Yeast mutants lacking telomerase are capable of maintaining telomeres by an alternate mechanism that depends on homologous recombination. We show here, by using Kluyveromyces lactis cells containing two types of telomeric repeats, that recombinational telomere elongation generates a repeating pattern common in most or all telomeres in survivors that retain both repeat types. We propose that these patterns arise from small circles of telomeric DNA being used as templates for rolling-circle gene conversion and that the sequence from the lengthened telomere is spread to other telomeres by additional, more typical gene conversion events. Consistent with this, artificially constructed circles of DNA containing telomeric repeats form long tandem arrays at telomeres when transformed into K. lactis cells. Mixing experiments done with two species of telomeric circles indicated that all of the integrated copies of the transforming sequence arise from a single original circular molecule.

* Corresponding author. Mailing address: Department of Genetics, Life Sciences Building, C318, University of Georgia, Athens, GA 30602-7223. Phone: (706) 542-4134. Fax: (706) 542-3910. E-mail: mjm{at}arches.uga.edu.

Molecular and Cellular Biology, July 2002, p. 4512-4521, Vol. 22, No. 13
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.13.4512-4521.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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