This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, P.-Y. J.
Right arrow Articles by Winston, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, P.-Y. J.
Right arrow Articles by Winston, F.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 2002, p. 5367-5379, Vol. 22, No. 15
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.15.5367-5379.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex

Pei-Yun Jenny Wu and Fred Winston*

Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115

Received 15 March 2002/ Returned for modification 25 April 2002/ Accepted 2 May 2002

The Saccharomyces cerevisiae SAGA complex is required for the normal transcription of a large number of genes. Complex integrity depends on three core subunits, Spt7, Spt20, and Ada1. We have investigated the role of Spt7 in the assembly and function of SAGA. Our results show that Spt7 is important in controlling the levels of the other core subunits and therefore of SAGA. In addition, partial SAGA complexes containing Spt7 can be assembled in the absence of both Spt20 and Ada1. Through biochemical and genetic analyses of a series of spt7 deletion mutants, we have identified a region of Spt7 required for interaction with the SAGA component Spt8. An adjacent Spt7 domain was found to be required for a processed form of Spt7 that is present in a previously identified altered form of SAGA called SLIK, SAGAalt, or SALSA. Analysis of an spt7 mutant with greatly reduced levels of SLIK/SAGAalt/SALSA suggests a subtle role for this complex in transcription that may be redundant with a subset of SAGA functions.

* Corresponding author. Mailing address: Department of Genetics, Harvard Medical School, 200 Longwood Ave., Boston, MA 02115. Phone: (617) 432-7768. Fax: (617) 432-3993. E-mail: winston{at}rascal.med.harvard.edu.

Molecular and Cellular Biology, August 2002, p. 5367-5379, Vol. 22, No. 15
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.15.5367-5379.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jacobson, S., Pillus, L. (2009). The SAGA Subunit Ada2 Functions in Transcriptional Silencing. Mol. Cell. Biol. 29: 6033-6045 [Abstract] [Full Text]  
  • Chang, C. S., Pillus, L. (2009). Collaboration Between the Essential Esa1 Acetyltransferase and the Rpd3 Deacetylase Is Mediated by H4K12 Histone Acetylation in Saccharomyces cerevisiae. Genetics 183: 149-160 [Abstract] [Full Text]  
  • Nagy, Z., Riss, A., Romier, C., le Guezennec, X., Dongre, A. R., Orpinell, M., Han, J., Stunnenberg, H., Tora, L. (2009). The Human SPT20-Containing SAGA Complex Plays a Direct Role in the Regulation of Endoplasmic Reticulum Stress-Induced Genes. Mol. Cell. Biol. 29: 1649-1660 [Abstract] [Full Text]  
  • Helmlinger, D., Marguerat, S., Villen, J., Gygi, S. P., Bahler, J., Winston, F. (2008). The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8. Genes Dev. 22: 3184-3195 [Abstract] [Full Text]  
  • Mohibullah, N., Hahn, S. (2008). Site-specific cross-linking of TBP in vivo and in vitro reveals a direct functional interaction with the SAGA subunit Spt3. Genes Dev. 22: 2994-3006 [Abstract] [Full Text]  
  • Liu, X., Vorontchikhina, M., Wang, Y.-L., Faiola, F., Martinez, E. (2008). STAGA Recruits Mediator to the MYC Oncoprotein To Stimulate Transcription and Cell Proliferation. Mol. Cell. Biol. 28: 108-121 [Abstract] [Full Text]  
  • Laprade, L., Rose, D., Winston, F. (2007). Characterization of New Spt3 and TATA-Binding Protein Mutants of Saccharomyces cerevisiae: Spt3 TBP Allele-Specific Interactions and Bypass of Spt8. Genetics 177: 2007-2017 [Abstract] [Full Text]  
  • Jiang, L., Smith, J. N., Anderson, S. L., Ma, P., Mizzen, C. A., Kelleher, N. L. (2007). Global Assessment of Combinatorial Post-translational Modification of Core Histones in Yeast Using Contemporary Mass Spectrometry: LYS4 TRIMETHYLATION CORRELATES WITH DEGREE OF ACETYLATION ON THE SAME H3 TAIL. J. Biol. Chem. 282: 27923-27934 [Abstract] [Full Text]  
  • James, N., Landrieux, E., Collart, M. A. (2007). A SAGA-Independent Function of SPT3 Mediates Transcriptional Deregulation in a Mutant of the Ccr4-Not Complex in Saccharomyces cerevisiae. Genetics 177: 123-135 [Abstract] [Full Text]  
  • Luthra, R., Kerr, S. C., Harreman, M. T., Apponi, L. H., Fasken, M. B., Ramineni, S., Chaurasia, S., Valentini, S. R., Corbett, A. H. (2007). Actively Transcribed GAL Genes Can Be Physically Linked to the Nuclear Pore by the SAGA Chromatin Modifying Complex. J. Biol. Chem. 282: 3042-3049 [Abstract] [Full Text]  
  • Ruault, M., Pillus, L. (2006). Chromatin-Modifiying Enzymes Are Essential When the Saccharomyces cerevisiae Morphogenesis Checkpoint Is Constitutively Activated. Genetics 174: 1135-1149 [Abstract] [Full Text]  
  • Chandy, M., Gutierrez, J. L., Prochasson, P., Workman, J. L. (2006). SWI/SNF Displaces SAGA-Acetylated Nucleosomes.. Eukaryot Cell 5: 1738-1747 [Abstract] [Full Text]  
  • Kohler, A., Pascual-Garcia, P., Llopis, A., Zapater, M., Posas, F., Hurt, E., Rodriguez-Navarro, S. (2006). The mRNA Export Factor Sus1 Is Involved in Spt/Ada/Gcn5 Acetyltransferase-mediated H2B Deubiquitinylation through Its Interaction with Ubp8 and Sgf11. Mol. Biol. Cell 17: 4228-4236 [Abstract] [Full Text]  
  • Hassan, A. H., Awad, S., Prochasson, P. (2006). The Swi2/Snf2 Bromodomain Is Required for the Displacement of SAGA and the Octamer Transfer of SAGA-acetylated Nucleosomes. J. Biol. Chem. 281: 18126-18134 [Abstract] [Full Text]  
  • Govind, C. K., Yoon, S., Qiu, H., Govind, S., Hinnebusch, A. G. (2005). Simultaneous Recruitment of Coactivators by Gcn4p Stimulates Multiple Steps of Transcription In Vivo. Mol. Cell. Biol. 25: 5626-5638 [Abstract] [Full Text]  
  • Soutoglou, E., Demeny, M. A., Scheer, E., Fienga, G., Sassone-Corsi, P., Tora, L. (2005). The Nuclear Import of TAF10 Is Regulated by One of Its Three Histone Fold Domain-Containing Interaction Partners. Mol. Cell. Biol. 25: 4092-4104 [Abstract] [Full Text]  
  • Qiu, H., Hu, C., Zhang, F., Hwang, G. J., Swanson, M. J., Boonchird, C., Hinnebusch, A. G. (2005). Interdependent Recruitment of SAGA and Srb Mediator by Transcriptional Activator Gcn4p. Mol. Cell. Biol. 25: 3461-3474 [Abstract] [Full Text]  
  • Prather, D. M., Larschan, E., Winston, F. (2005). Evidence that the Elongation Factor TFIIS Plays a Role in Transcription Initiation at GAL1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 2650-2659 [Abstract] [Full Text]  
  • Ingvarsdottir, K., Krogan, N. J., Emre, N. C. T., Wyce, A., Thompson, N. J., Emili, A., Hughes, T. R., Greenblatt, J. F., Berger, S. L. (2005). H2B Ubiquitin Protease Ubp8 and Sgf11 Constitute a Discrete Functional Module within the Saccharomyces cerevisiae SAGA Complex. Mol. Cell. Biol. 25: 1162-1172 [Abstract] [Full Text]  
  • Larschan, E., Winston, F. (2005). The Saccharomyces cerevisiae Srb8-Srb11 Complex Functions with the SAGA Complex during Gal4-Activated Transcription. Mol. Cell. Biol. 25: 114-123 [Abstract] [Full Text]  
  • Game, J. C., Williamson, M. S., Baccari, C. (2005). X-Ray Survival Characteristics and Genetic Analysis for Nine Saccharomyces Deletion Mutants That Show Altered Radiation Sensitivity. Genetics 169: 51-63 [Abstract] [Full Text]  
  • Powell, D. W., Weaver, C. M., Jennings, J. L., McAfee, K. J., He, Y., Weil, P. A., Link, A. J. (2004). Cluster Analysis of Mass Spectrometry Data Reveals a Novel Component of SAGA. Mol. Cell. Biol. 24: 7249-7259 [Abstract] [Full Text]  
  • Warfield, L., Ranish, J. A., Hahn, S. (2004). Positive and negative functions of the SAGA complex mediated through interaction of Spt8 with TBP and the N-terminal domain of TFIIA. Genes Dev. 18: 1022-1034 [Abstract] [Full Text]  
  • Aouida, M., Page, N., Leduc, A., Peter, M., Ramotar, D. (2004). A Genome-Wide Screen in Saccharomyces cerevisiae Reveals Altered Transport As a Mechanism of Resistance to the Anticancer Drug Bleomycin. Cancer Res. 64: 1102-1109 [Abstract] [Full Text]  
  • Daniel, J. A., Torok, M. S., Sun, Z.-W., Schieltz, D., Allis, C. D., Yates, J. R. III, Grant, P. A. (2004). Deubiquitination of Histone H2B by a Yeast Acetyltransferase Complex Regulates Transcription. J. Biol. Chem. 279: 1867-1871 [Abstract] [Full Text]  
  • Hess, D., Liu, B., Roan, N. R., Sternglanz, R., Winston, F. (2004). Spt10-Dependent Transcriptional Activation in Saccharomyces cerevisiae Requires both the Spt10 Acetyltransferase Domain and Spt21. Mol. Cell. Biol. 24: 135-143 [Abstract] [Full Text]  
  • Yoon, S., Qiu, H., Swanson, M. J., Hinnebusch, A. G. (2003). Recruitment of SWI/SNF by Gcn4p Does Not Require Snf2p or Gcn5p but Depends Strongly on SWI/SNF Integrity, SRB Mediator, and SAGA. Mol. Cell. Biol. 23: 8829-8845 [Abstract] [Full Text]  
  • Henry, K. W., Wyce, A., Lo, W.-S., Duggan, L. J., Emre, N.C. T., Kao, C.-F., Pillus, L., Shilatifard, A., Osley, M. A., Berger, S. L. (2003). Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes Dev. 17: 2648-2663 [Abstract] [Full Text]  
  • Liu, X., Tesfai, J., Evrard, Y. A., Dent, S. Y. R., Martinez, E. (2003). c-Myc Transformation Domain Recruits the Human STAGA Complex and Requires TRRAP and GCN5 Acetylase Activity for Transcription Activation. J. Biol. Chem. 278: 20405-20412 [Abstract] [Full Text]  
  • Barbaric, S., Reinke, H., Horz, W. (2003). Multiple Mechanistically Distinct Functions of SAGA at the PHO5 Promoter. Mol. Cell. Biol. 23: 3468-3476 [Abstract] [Full Text]  
  • Pray-Grant, M. G., Schieltz, D., McMahon, S. J., Wood, J. M., Kennedy, E. L., Cook, R. G., Workman, J. L., Yates III, J. R., Grant, P. A. (2002). The Novel SLIK Histone Acetyltransferase Complex Functions in the Yeast Retrograde Response Pathway. Mol. Cell. Biol. 22: 8774-8786 [Abstract] [Full Text]  
  • Sterner, D. E., Belotserkovskaya, R., Berger, S. L. (2002). SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription. Proc. Natl. Acad. Sci. USA 99: 11622-11627 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»