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Molecular and Cellular Biology, August 2002, p. 5708-5720, Vol. 22, No. 16
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.16.5708-5720.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Steady-State Nuclear Localization of Exportin-t Involves RanGTP Binding and Two Distinct Nuclear Pore Complex Interaction Domains

Scott Kuersten,,{dagger} Gert-Jan Arts,,{ddagger} Tobias C. Walther, Ludwig Englmeier,,§ and Iain W. Mattaj*

Gene Expression Programme, European Molecular Biology Laboratory, D-69117 Heidelberg, Germany

Received 7 March 2002/ Returned for modification 15 April 2002/ Accepted 13 May 2002

Vertebrate tRNA export receptor exportin-t (Xpo-t) binds to RanGTP and mature tRNAs cooperatively to form a nuclear export complex. Xpo-t shuttles bidirectionally through nuclear pore complexes (NPCs) but is mainly nuclear at steady state. The steady-state distribution of Xpo-t is shown to depend on its interaction with RanGTP. Two distinct Xpo-t NPC interaction domains that bind differentially to peripherally localized nucleoporins in vitro are identified. The N terminus binds to both Nup153 and RanBP2/Nup358 in a RanGTP-dependent manner, while the C terminus binds to CAN/Nup214 independently of Ran. We propose that these interactions increase the concentration of tRNA export complexes and of empty Xpo-t in the vicinity of NPCs and thus increase the efficiency of the Xpo-t transport cycle.

* Corresponding author. Mailing address: Gene Expression Programme, European Molecular Biology Laboratory, Meyhofstr.1, D-69117 Heidelberg, Germany. Phone: 49 6221 38 73 93. Fax: 49 6221 38 75 18. E-mail: mattaj{at}embl-heidelberg.de.

{dagger} Present address: Department of Genetics, University of Wisconsin-Madison, Madison, WI 53706.

{ddagger} Present address: Galapagos Genomics B.V., 2301 CA Leiden, The Netherlands.

§ Present address: von-Prant-Str. 4d, 83043 Bad Aibling, Germany.

Molecular and Cellular Biology, August 2002, p. 5708-5720, Vol. 22, No. 16
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.16.5708-5720.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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