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Molecular and Cellular Biology, September 2002, p. 6384-6392, Vol. 22, No. 18
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.18.6384-6392.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of RAD51-Independent Break-Induced Replication That Acts Preferentially with Short Homologous Sequences

Grzegorz Ira,{dagger} and James E. Haber*

Rosenstiel Center and Department of Biology, Brandeis University, Waltham, Massachussetts 02454-9110

Received 19 April 2002/ Returned for modification 3 June 2002/ Accepted 24 June 2002

Repair of double-strand breaks by gene conversions between homologous sequences located on different Saccharomyces cerevisiae chromosomes or plasmids requires RAD51. When repair occurs between inverted repeats of the same plasmid, both RAD51-dependent and RAD51-independent repairs are found. Completion of RAD51-independent plasmid repair events requires RAD52, RAD50, RAD59, TID1 (RDH54), and SRS2 and appears to involve break-induced replication coupled to single-strand annealing. Surprisingly, RAD51-independent recombination requires much less homology (30 bp) for strand invasion than does RAD51-dependent repair (approximately 100 bp); in fact, the presence of Rad51p impairs recombination with short homology. The differences between the RAD51- and RAD50/RAD59-dependent pathways account for the distinct ways that two different recombination processes maintain yeast telomeres in the absence of telomerase.

* Corresponding author. Mailing address: Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02454-9110. Phone: (781) 736-2462. Fax: (781) 736-2405. E-mail: haber{at}brandeis.edu.

{dagger} Permanent address: Institute of General and Molecular Biology, Nicholas Copernicus University, 87-100 Torun, Poland.

Molecular and Cellular Biology, September 2002, p. 6384-6392, Vol. 22, No. 18
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.18.6384-6392.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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