This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pak, J. Articles by Segall, J. Search for Related Content PubMed PubMed Citation Articles by Pak, J. Articles by Segall, J.

Regulation of the Premiddle and Middle Phases of Expression of the NDT80 Gene during Sporulation of Saccharomyces cerevisiae

Department of Molecular and Medical Genetics,¹ Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada M5S 1A8²

Received 5 April 2002/ Returned for modification 7 June 2002/ Accepted 21 June 2002

The NDT80 gene of Saccharomyces cerevisiae, which encodes a global activator of transcription of middle sporulation-specific genes, is first expressed after the activation of early meiotic genes but prior to activation of middle sporulation-specific genes. Both upstream repression sequence 1 (URS1) and mid-sporulation element (MSE) sites are present in the promoter region of the NDT80 gene; these elements have been shown previously to contribute to the regulation of expression of early and middle sporulation-specific genes, respectively, by mediating repression in growing cells and activation at specific times during sporulation. In this study, we have shown that the overlapping windows of URS1- and MSE-mediated repression and activation are responsible for the distinctive premiddle expression pattern of the NDT80 gene. Our data suggest that a Sum1-associated repression complex bound at the NDT80 MSE sites prevents Ime1 tethered at the NDT80 URS1 sites from activating transcription of the NDT80 gene at the time that Ime1-dependent activation of early URS1-regulated meiotic genes is occurring. We propose that a decrease in the efficiency of Sum1-mediated repression as cells progress through the early events of the sporulation program allows the previously inactive Ime1 tethered at the URS1^NDT80 sites to promote a low level of expression of the NDT80 gene. This initial phase of URS1-dependent NDT80 expression is followed by Ndt80-dependent upregulation of its own expression, which requires the MSE^NDT80 sites and occurs concomitantly with Ndt80-dependent activation of a set of middle MSE-regulated sporulation-specific genes. Mutation of IME2 prevents expression of NDT80 in sporulating cells. We show in this study that NDT80 is expressed and that middle genes are activated in cells of an ime2/ime2 sum1/sum1 strain in sporulation medium. This suggests that Ime2 activates expression of NDT80 by eliminating Sum1-mediated repression.

This article has been cited by other articles:

• Schlecht, U., Erb, I., Demougin, P., Robine, N., Borde, V., Nimwegen, E. v., Nicolas, A., Primig, M. (2008). Genome-wide Expression Profiling, In Vivo DNA Binding Analysis, and Probabilistic Motif Prediction Reveal Novel Abf1 Target Genes during Fermentation, Respiration, and Sporulation in Yeast. Mol. Biol. Cell 19: 2193-2207 [Abstract] [Full Text]  
• Wang, J.-S., Yang, Y.-L., Wu, C.-J., Ouyang, K. J., Tseng, K.-Y., Chen, C.-G., Wang, H., Lo, H.-J. (2006). The DNA-binding domain of CaNdt80p is required to activate CDR1 involved in drug resistance in Candida albicans.. J Med Microbiol 55: 1403-1411 [Abstract] [Full Text]  
• Schindler, K., Winter, E. (2006). Phosphorylation of Ime2 Regulates Meiotic Progression in Saccharomyces cerevisiae. J. Biol. Chem. 281: 18307-18316 [Abstract] [Full Text]  
• Neiman, A. M. (2005). Ascospore Formation in the Yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 69: 565-584 [Abstract] [Full Text]  
• Xing, B., van der Laan, M. J. (2005). A causal inference approach for constructing transcriptional regulatory networks. Bioinformatics 21: 4007-4013 [Abstract] [Full Text]  
• McDonald, C. M., Cooper, K. F., Winter, E. (2005). The Ama1-Directed Anaphase-Promoting Complex Regulates the Smk1 Mitogen-Activated Protein Kinase During Meiosis in Yeast. Genetics 171: 901-911 [Abstract] [Full Text]  
• Wang, W., Cherry, J. M., Nochomovitz, Y., Jolly, E., Botstein, D., Li, H. (2005). Inference of combinatorial regulation in yeast transcriptional networks: A case study of sporulation. Proc. Natl. Acad. Sci. USA 102: 1998-2003 [Abstract] [Full Text]  
• Garrido, E., Voss, U., Muller, P., Castillo-Lluva, S., Kahmann, R., Perez-Martin, J. (2004). The induction of sexual development and virulence in the smut fungus Ustilago maydis depends on Crk1, a novel MAPK protein. Genes Dev. 18: 3117-3130 [Abstract] [Full Text]  
• Malone, R. E., Haring, S. J., Foreman, K. E., Pansegrau, M. L., Smith, S. M., Houdek, D. R., Carpp, L., Shah, B., Lee, K. E. (2004). The Signal from the Initiation of Meiotic Recombination to the First Division of Meiosis. Eukaryot Cell 3: 598-609 [Abstract] [Full Text]  
• Fingerman, I. M., Sutphen, K., Montano, S. P., Georgiadis, M. M., Vershon, A. K. (2004). Characterization of critical interactions between Ndt80 and MSE DNA defining a novel family of Ig-fold transcription factors. Nucleic Acids Res 32: 2947-2956 [Abstract] [Full Text]  
• Pierce, M., Benjamin, K. R., Montano, S. P., Georgiadis, M. M., Winter, E., Vershon, A. K. (2003). Sum1 and Ndt80 Proteins Compete for Binding to Middle Sporulation Element Sequences That Control Meiotic Gene Expression. Mol. Cell. Biol. 23: 4814-4825 [Abstract] [Full Text]  
• Benjamin, K. R., Zhang, C., Shokat, K. M., Herskowitz, I. (2003). Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2. Genes Dev. 17: 1524-1539 [Abstract] [Full Text]  
• Honigberg, S. M., Purnapatre, K. (2003). Signal pathway integration in the switch from the mitotic cell cycle to meiosis in yeast. J. Cell Sci. 116: 2137-2147 [Abstract] [Full Text]