This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brandau, O.
Right arrow Articles by Fässler, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brandau, O.
Right arrow Articles by Fässler, R.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 2002, p. 6627-6635, Vol. 22, No. 18
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.18.6627-6635.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Chondromodulin I Is Dispensable during Enchondral Ossification and Eye Development

Oliver Brandau,1,2* Attila Aszódi,1,2 Ernst B. Hunziker,3 Peter J. Neame,4 Dietmar Vestweber,5,6 and Reinhard Fässler1,2

Department of Experimental Pathology, Lund University, S-22185 Lund, Sweden,1 Department of Molecular Medicine, Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany,2 M. E. Müller Institute for Biomechanics, University of Bern, 3010 Bern, Switzerland,3 Center for Research in Skeletal Development and Pediatric Orthopedics, Shriners Hospital for Children, Tampa, Florida 33612,4 Max-Planck- Institute for Vascular Biology, and Institute of Cell Biology,5 ZMBE, University of Münster D-48149 Münster, Germany6

Received 15 April 2002/ Accepted 29 May 2002

Chondromodulin I (chm-I), a type II transmembrane protein, is highly expressed in the avascular zones of cartilage but is downregulated in the hypertrophic region, which is invaded by blood vessels during enchondral ossification. In vitro and in vivo assays with the purified protein have shown chondrocyte-modulating and angiogenesis-inhibiting functions. To investigate chm-I function in vivo, we generated transgenic mice lacking chm-I mRNA and protein. Null mice are viable and fertile and show no morphological changes. No abnormalities in vascular invasion and cartilage development were detectable. No evidence was found for a compensating function of tendin, a recently published homologue highly expressed in tendons and also, at low levels, in cartilage. Furthermore, no differences in the expression of other angiogenic or antiangiogenic factors such as transforming growth factor ß1 (TGF-ß1), TGF-ß2, TGF-ß3, fibroblast growth factor 2, and vascular endothelial growth factor were found. The surprising lack of phenotype in the chm-I-deficient mice suggests either a different function for chm-I in vivo than has been proposed or compensatory changes in uninvestigated angiogenic or angiogenesis-inhibiting factors. Further analysis using double-knockout technology will be necessary to analyze the function of chm-I in the complex process of enchondral ossification.

* Corresponding author. Mailing address: Department of Molecular Medicine, Am Klopferspitz 18a, Martinsried, Germany. Phone: 49-89-8578-2420. Fax: 49-89-8578-2444. E-mail: brandau{at}biochem.mpg.de.

Molecular and Cellular Biology, September 2002, p. 6627-6635, Vol. 22, No. 18
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.18.6627-6635.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Docheva, D., Hunziker, E. B., Fassler, R., Brandau, O. (2005). Tenomodulin Is Necessary for Tenocyte Proliferation and Tendon Maturation. Mol. Cell. Biol. 25: 699-705 [Abstract] [Full Text]  
  • Ko, Y., Kobbe, B., Nicolae, C., Miosge, N., Paulsson, M., Wagener, R., Aszodi, A. (2004). Matrilin-3 Is Dispensable for Mouse Skeletal Growth and Development. Mol. Cell. Biol. 24: 1691-1699 [Abstract] [Full Text]  
  • Aszodi, A., Hunziker, E. B., Brakebusch, C., Fassler, R. (2003). {beta}1 integrins regulate chondrocyte rotation, G1 progression, and cytokinesis. Genes Dev. 17: 2465-2479 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»