This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miyamoto, T.
Right arrow Articles by Sugiura, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miyamoto, T.
Right arrow Articles by Sugiura, M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2002, p. 6726-6734, Vol. 22, No. 19
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.19.6726-6734.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Recognition of RNA Editing Sites Is Directed by Unique Proteins in Chloroplasts: Biochemical Identification of cis-Acting Elements and trans-Acting Factors Involved in RNA Editing in Tobacco and Pea Chloroplasts

Tetsuya Miyamoto,1 Junichi Obokata,1 and Masahiro Sugiura2*

Center for Gene Research, Nagoya University, Nagoya 464-8602,1 Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, Nagoya 467-8501, Japan2

Received 4 March 2002/ Returned for modification 13 May 2002/ Accepted 20 June 2002

RNA editing in higher-plant chloroplasts involves C-to-U conversions at specific sites. Although in vivo analyses have been performed, little is known about the biochemical aspects of chloroplast editing reactions. Here we improved our original in vitro system and devised a procedure for preparing active chloroplast extracts not only from tobacco plants but also from pea plants. Using our tobacco in vitro system, cis-acting elements were defined for psbE and petB mRNAs. Distinct proteins were found to bind specifically to each cis-element, a 56-kDa protein to the psbE site and a 70-kDa species to the petB site. Pea chloroplasts lack the corresponding editing site in psbE since T is already present in the DNA. Parallel in vitro analyses with tobacco and pea extracts revealed that the pea plant has no editing activity for psbE mRNAs and lacks the 56-kDa protein, whereas petB mRNAs are edited and the 70-kDa protein is also present. Therefore, coevolution of an editing site and its cognate trans-factor was demonstrated biochemically in psbE mRNA editing between tobacco and pea plants.

* Corresponding author. Mailing address: Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, Nagoya 467-8501, Japan. Phone: 81-52-872-6021. Fax: 81-52-872-6021. E-mail: sugiura{at}nsc.nagoya-cu.ac.jp.

Molecular and Cellular Biology, October 2002, p. 6726-6734, Vol. 22, No. 19
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.19.6726-6734.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Karcher, D., Bock, R. (2009). Identification of the chloroplast adenosine-to-inosine tRNA editing enzyme. RNA 15: 1251-1257 [Abstract] [Full Text]  
  • Zehrmann, A., Verbitskiy, D., van der Merwe, J. A., Brennicke, A., Takenaka, M. (2009). A DYW Domain-Containing Pentatricopeptide Repeat Protein Is Required for RNA Editing at Multiple Sites in Mitochondria of Arabidopsis thaliana. Plant Cell 21: 558-567 [Abstract] [Full Text]  
  • Okuda, K., Habata, Y., Kobayashi, Y., Shikanai, T. (2008). Amino acid sequence variations in Nicotiana CRR4 orthologs determine the species-specific efficiency of RNA editing in plastids. Nucleic Acids Res 36: 6155-6164 [Abstract] [Full Text]  
  • Verbitskiy, D., van der Merwe, J. A., Zehrmann, A., Brennicke, A., Takenaka, M. (2008). Multiple Specificity Recognition Motifs Enhance Plant Mitochondrial RNA Editing in Vitro. J. Biol. Chem. 283: 24374-24381 [Abstract] [Full Text]  
  • Rudinger, M., Polsakiewicz, M., Knoop, V. (2008). Organellar RNA Editing and Plant-Specific Extensions of Pentatricopeptide Repeat Proteins in Jungermanniid but not in Marchantiid Liverworts. Mol Biol Evol 25: 1405-1414 [Abstract] [Full Text]  
  • Heller, W. P., Hayes, M. L., Hanson, M. R. (2008). Cross-competition in Editing of Chloroplast RNA Transcripts in Vitro Implicates Sharing of Trans-factors between Different C Targets. J. Biol. Chem. 283: 7314-7319 [Abstract] [Full Text]  
  • Bentolila, S., Elliott, L. E., Hanson, M. R. (2008). Genetic Architecture of Mitochondrial Editing in Arabidopsis thaliana. Genetics 178: 1693-1708 [Abstract] [Full Text]  
  • Karcher, D., Kahlau, S., Bock, R. (2008). Faithful editing of a tomato-specific mRNA editing site in transgenic tobacco chloroplasts. RNA 14: 217-224 [Abstract] [Full Text]  
  • Kobayashi, Y., Matsuo, M., Sakamoto, K., Wakasugi, T., Yamada, K., Obokata, J. (2008). Two RNA editing sites with cis-acting elements of moderate sequence identity are recognized by an identical site-recognition protein in tobacco chloroplasts. Nucleic Acids Res 36: 311-318 [Abstract] [Full Text]  
  • Mulligan, R. M., Chang, K. L. C., Chou, C. C. (2007). Computational Analysis of RNA Editing Sites in Plant Mitochondrial Genomes Reveals Similar Information Content and a Sporadic Distribution of Editing Sites. Mol Biol Evol 24: 1971-1981 [Abstract] [Full Text]  
  • Hayes, M. L., Hanson, M. R. (2007). Identification of a sequence motif critical for editing of a tobacco chloroplast transcript. RNA 13: 281-288 [Abstract] [Full Text]  
  • Okuda, K., Nakamura, T., Sugita, M., Shimizu, T., Shikanai, T. (2006). A Pentatricopeptide Repeat Protein Is a Site Recognition Factor in Chloroplast RNA Editing. J. Biol. Chem. 281: 37661-37667 [Abstract] [Full Text]  
  • Tillich, M., Lehwark, P., Morton, B. R., Maier, U. G. (2006). The Evolution of Chloroplast RNA Editing. Mol Biol Evol 23: 1912-1921 [Abstract] [Full Text]  
  • Hayes, M. L., Reed, M. L., Hegeman, C. E., Hanson, M. R. (2006). Sequence elements critical for efficient RNA editing of a tobacco chloroplast transcript in vivo and in vitro. Nucleic Acids Res 34: 3742-3754 [Abstract] [Full Text]  
  • Bentolila, S., Chateigner-Boutin, A.-L., Hanson, M. R. (2005). Ecotype Allelic Variation in C-to-U Editing Extent of a Mitochondrial Transcript Identifies RNA-Editing Quantitative Trait Loci in Arabidopsis. Plant Physiol. 139: 2006-2016 [Abstract] [Full Text]  
  • NEUWIRT, J., TAKENAKA, M., VAN DER MERWE, J. A., BRENNICKE, A. (2005). An in vitro RNA editing system from cauliflower mitochondria: Editing site recognition parameters can vary in different plant species. RNA 11: 1563-1570 [Abstract] [Full Text]  
  • Schmitz-Linneweber, C., Kushnir, S., Babiychuk, E., Poltnigg, P., Herrmann, R. G., Maier, R. M. (2005). Pigment Deficiency in Nightshade/Tobacco Cybrids Is Caused by the Failure to Edit the Plastid ATPase {alpha}-Subunit mRNA. Plant Cell 17: 1815-1828 [Abstract] [Full Text]  
  • Hegeman, C. E., Halter, C. P., Owens, T. G., Hanson, M. R. (2005). Expression of complementary RNA from chloroplast transgenes affects editing efficiency of transgene and endogenous chloroplast transcripts. Nucleic Acids Res 33: 1454-1464 [Abstract] [Full Text]  
  • MARIS, C., MASSE, J., CHESTER, A., NAVARATNAM, N., ALLAIN, F. H.-T. (2005). NMR structure of the apoB mRNA stem-loop and its interaction with the C to U editing APOBEC1 complementary factor. RNA 11: 173-186 [Abstract] [Full Text]  
  • Choury, D., Farre, J.-C., Jordana, X., Araya, A. (2004). Different patterns in the recognition of editing sites in plant mitochondria. Nucleic Acids Res 32: 6397-6406 [Abstract] [Full Text]  
  • Inada, M., Sasaki, T., Yukawa, M., Tsudzuki, T., Sugiura, M. (2004). A Systematic Search for RNA Editing Sites in Pea Chloroplasts: an Editing Event Causes Diversification from the Evolutionarily Conserved Amino Acid Sequence. Plant Cell Physiol 45: 1615-1622 [Abstract] [Full Text]  
  • Takenaka, M., Neuwirt, J., Brennicke, A. (2004). Complex cis-elements determine an RNA editing site in pea mitochondria. Nucleic Acids Res 32: 4137-4144 [Abstract] [Full Text]  
  • Lurin, C., Andres, C., Aubourg, S., Bellaoui, M., Bitton, F., Bruyere, C., Caboche, M., Debast, C., Gualberto, J., Hoffmann, B., Lecharny, A., Le Ret, M., Martin-Magniette, M.-L., Mireau, H., Peeters, N., Renou, J.-P., Szurek, B., Taconnat, L., Small, I. (2004). Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis. Plant Cell 16: 2089-2103 [Abstract] [Full Text]  
  • Miyamoto, T., Obokata, J., Sugiura, M. (2004). A site-specific factor interacts directly with its cognate RNA editing site in chloroplast transcripts. Proc. Natl. Acad. Sci. USA 101: 48-52 [Abstract] [Full Text]  
  • Takenaka, M., Brennicke, A. (2003). In Vitro RNA Editing in Pea Mitochondria Requires NTP or dNTP, Suggesting Involvement of an RNA Helicase. J. Biol. Chem. 278: 47526-47533 [Abstract] [Full Text]  
  • Sasaki, T., Yukawa, Y., Miyamoto, T., Obokata, J., Sugiura, M. (2003). Identification of RNA Editing Sites in Chloroplast Transcripts from the Maternal and Paternal Progenitors of Tobacco (Nicotiana tabacum): Comparative Analysis Shows the Involvement of Distinct Trans-Factors for ndhB Editing. Mol Biol Evol 20: 1028-1035 [Abstract] [Full Text]  
  • Chateigner-Boutin, A.-L., Hanson, M. R. (2003). Developmental co-variation of RNA editing extent of plastid editing sites exhibiting similar cis-elements. Nucleic Acids Res 31: 2586-2594 [Abstract] [Full Text]  
  • Kugita, M., Yamamoto, Y., Fujikawa, T., Matsumoto, T., Yoshinaga, K. (2003). RNA editing in hornwort chloroplasts makes more than half the genes functional. Nucleic Acids Res 31: 2417-2423 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»