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Molecular and Cellular Biology, January 2002, p. 421-429, Vol. 22, No. 2
0270-7306/01/$04.00+0     DOI: 10.1128/MCB.22.2.421-429.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

hpr1{Delta} Affects Ribosomal DNA Recombination and Cell Life Span in Saccharomyces cerevisiae

Robert J. Merker and Hannah L. Klein*

Department of Biochemistry and Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, New York 10016

Received 19 June 2001/ Returned for modification 30 July 2001/ Accepted 8 October 2001

Multiple genetic pathways have been shown to regulate life span and aging in the yeast Saccharomyces cerevisiae. Here we show that loss of a component of the RNA polymerase II complex, Hpr1p, results in a decreased life span. Although hpr1{Delta} mutants have an increased rate of recombination within the ribosomal DNA (rDNA) array, this is not accompanied by an increase in extrachromosomal rDNA circles (ERCs). Analyses of mutants that affect replication of the rDNA array and suppressors that reverse the phenotypes of the hpr1{Delta} mutant show that the reduced life span is associated with increased genomic instability but not with increased ERC formation. The hpr1{Delta} mutant acts in a pathway distinct from previously described mutants that reduce life span.

* Corresponding author. Mailing address: Department of Biochemistry, NYU School of Medicine, 550 First Ave., New York, NY 10016. Phone: (212) 263-5778. Fax: (212) 263-8166. E-mail: hannah.klein{at}med.nyu.edu.

Molecular and Cellular Biology, January 2002, p. 421-429, Vol. 22, No. 2
0022-538X/01/$04.00+0     DOI: 10.1128/MCB.22.2.421-429.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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