This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Aladjem, M. I. Articles by Wahl, G. M. Search for Related Content PubMed PubMed Citation Articles by Aladjem, M. I. Articles by Wahl, G. M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2002, p. 442-452, Vol. 22, No. 2
0270-7306/01/$04.00+0     DOI: 10.1128/MCB.22.2.442-452.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Replication Initiation Patterns in the ß-Globin Loci of Totipotent and Differentiated Murine Cells: Evidence for Multiple Initiation Regions

Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, Maryland 20892-4255,¹ Gene Expression Laboratory, The Salk Institute,² Fred Hutchinson Cancer Research Center, Seattle, Washington 98109,³ Arizona Cancer Center/BMT, Tucson, Arizona 85724⁴

Received 20 August 2001/ Returned for modification 19 September 2001/ Accepted

The replication initiation pattern of the murine ß-globin locus was analyzed in totipotent embryonic stem cells and in differentiated cell lines. Initiation events in the murine ß-globin locus were detected in a region extending from the embryonic Ey gene to the adult ßminor gene, unlike the restricted initiation observed in the human locus. Totipotent and differentiated cells exhibited similar initiation patterns. Deletion of the region between the adult globin genes did not prevent initiation in the remainder of the locus, suggesting that the potential to initiate DNA replication was not contained exclusively within the primary sequence of the deleted region. In addition, a deletion encompassing the six identified 5' hypersensitive sites in the mouse locus control region had no effect on initiation from within the locus. As this deletion also did not affect the chromatin structure of the locus, we propose that the sequences determining both chromatin structure and replication initiation lie outside the hypersensitive sites removed by the deletion.

This article has been cited by other articles:

• Wang, L., Lin, C. M., Lopreiato, J. O., Aladjem, M. I. (2006). Cooperative sequence modules determine replication initiation sites at the human {beta}-globin locus. Hum Mol Genet 15: 2613-2622 [Abstract] [Full Text]  
• Rowntree, R. K., Lee, J. T. (2006). Mapping of DNA Replication Origins to Noncoding Genes of the X-Inactivation Center. Mol. Cell. Biol. 26: 3707-3717 [Abstract] [Full Text]  
• Hayashida, T., Oda, M., Ohsawa, K., Yamaguchi, A., Hosozawa, T., Locksley, R. M., Giacca, M., Masai, H., Miyatake, S. (2006). Replication Initiation from a Novel Origin Identified in the Th2 Cytokine Cluster Locus Requires a Distant Conserved Noncoding Sequence. J. Immunol. 176: 5446-5454 [Abstract] [Full Text]  
• Takeda, D. Y., Shibata, Y., Parvin, J. D., Dutta, A. (2005). Recruitment of ORC or CDC6 to DNA is sufficient to create an artificial origin of replication in mammalian cells. Genes Dev. 19: 2827-2836 [Abstract] [Full Text]  
• Buzina, A., Aladjem, M. I., Kolman, J. L., Wahl, G. M., Ellis, J. (2005). Initiation of DNA replication at the human {beta}-globin 3' enhancer. Nucleic Acids Res 33: 4412-4424 [Abstract] [Full Text]  
• Reyes, A., Yang, M. Y., Bowmaker, M., Holt, I. J. (2005). Bidirectional Replication Initiates at Sites Throughout the Mitochondrial Genome of Birds. J. Biol. Chem. 280: 3242-3250 [Abstract] [Full Text]  
• Kemp, M. G., Ghosh, M., Liu, G., Leffak, M. (2005). The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells. Nucleic Acids Res 33: 325-336 [Abstract] [Full Text]  
• Hiratani, I., Leskovar, A., Gilbert, D. M. (2004). Differentiation-induced replication-timing changes are restricted to AT-rich/long interspersed nuclear element (LINE)-rich isochores. Proc. Natl. Acad. Sci. USA 101: 16861-16866 [Abstract] [Full Text]  
• Wang, L., Lin, C.-M., Brooks, S., Cimbora, D., Groudine, M., Aladjem, M. I. (2004). The Human {beta}-Globin Replication Initiation Region Consists of Two Modular Independent Replicators. Mol. Cell. Biol. 24: 3373-3386 [Abstract] [Full Text]  
• Bowmaker, M., Yang, M. Y., Yasukawa, T., Reyes, A., Jacobs, H. T., Huberman, J. A., Holt, I. J. (2003). Mammalian Mitochondrial DNA Replicates Bidirectionally from an Initiation Zone. J. Biol. Chem. 278: 50961-50969 [Abstract] [Full Text]  
• Prioleau, M.-N., Gendron, M.-C., Hyrien, O. (2003). Replication of the Chicken {beta}-Globin Locus: Early-Firing Origins at the 5' HS4 Insulator and the {rho}- and {beta}A-Globin Genes Show Opposite Epigenetic Modifications. Mol. Cell. Biol. 23: 3536-3549 [Abstract] [Full Text]  
• Nenguke, T., Aladjem, M. I., Gusella, J. F., Wexler, N. S., The Venezuela HD Project, , Arnheim, N. (2003). Candidate DNA replication initiation regions at human trinucleotide repeat disease loci. Hum Mol Genet 12: 1021-1028 [Abstract] [Full Text]  
• Liu, G., Malott, M., Leffak, M. (2003). Multiple Functional Elements Comprise a Mammalian Chromosomal Replicator. Mol. Cell. Biol. 23: 1832-1842 [Abstract] [Full Text]  
• Zhou, J., Ashouian, N., Delepine, M., Matsuda, F., Chevillard, C., Riblet, R., Schildkraut, C. L., Birshtein, B. K. (2002). The origin of a developmentally regulated Igh replicon is located near the border of regulatory domains for Igh replication and expression. Proc. Natl. Acad. Sci. USA 99: 13693-13698 [Abstract] [Full Text]