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Molecular and Cellular Biology, January 2002, p. 517-524, Vol. 22, No. 2
0270-7306/01/$04.00+0     DOI: 10.1128/MCB.22.2.517-524.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

PAS Domain-Mediated WC-1/WC-2 Interaction Is Essential for Maintaining the Steady-State Level of WC-1 and the Function of Both Proteins in Circadian Clock and Light Responses of Neurospora

Ping Cheng,1 Yuhong Yang,1 Kevin H. Gardner,2 and Yi Liu1*

Department of Physiology,1 Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 753902

Received 15 August 2001/ Returned for modification 19 October 2001/ Accepted 25 October 2001

In the frq-wc-based circadian feedback loops of Neurospora, two PAS domain-containing transcription factors, WHITE COLLAR-1 (WC-1) and WC-2, form heterodimeric complexes that activate the transcription of frequency (frq). FRQ serves two roles in these feedback loops: repressing its own transcription by interacting with the WC complex and positively upregulating the levels of WC-1 and WC-2 proteins. We report here that the steady-state level of WC-1 protein is independently regulated by both FRQ and WC-2 through different posttranscriptional mechanisms. The WC-1 level is extremely low in wc-2 knockout strains, and this low level of expression is independent of wc-1 transcription and FRQ protein expression. In addition, our data show that the PAS domain of WC-2 mediates the interactions of this protein with both WC-1 and FRQ in vivo. Such interactions are essential for maintaining the steady-state level of WC-1 and the proper function of WC-1 and WC-2 in circadian clock and light responses.

* Corresponding author. Mailing address: University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390. Phone: (214) 648-3701. Fax: (214) 648-7891. E-mail: Yi.Liu{at}UTsouthwestern.edu.

Molecular and Cellular Biology, January 2002, p. 517-524, Vol. 22, No. 2
0022-538X/01/$04.00+0     DOI: 10.1128/MCB.22.2.517-524.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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