This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sopko, R.
Right arrow Articles by Stuart, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sopko, R.
Right arrow Articles by Stuart, D.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 2002, p. 7024-7040, Vol. 22, No. 20
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.20.7024-7040.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phosphorylation and Maximal Activity of Saccharomyces cerevisiae Meiosis-Specific Transcription Factor Ndt80 Is Dependent on Ime2

Richelle Sopko,{dagger} Sheetal Raithatha, and David Stuart*

Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7

Received 12 March 2002/ Returned for modification 16 April 2002/ Accepted 18 July 2002

The Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80 is responsible for the induction of a class of genes referred to as middle sporulation genes. Among the members of this family are the B-type cyclins and other genes whose products are required for meiotic chromosome division and spore morphogenesis. Inactivation of NDT80 leads to a failure to induce the middle sporulation genes and a subsequent arrest in pachytene. The expression of NDT80 is itself highly regulated. The initial transcription of NDT80 is dependent upon the protein kinase Ime2; once Ndt80 protein accumulates, it activates its own promoter, thus generating an autoactivation loop. In addition to being transcriptionally regulated, Ndt80 protein is posttranslationally regulated. Phosphorylation of Ndt80 occurs coincident with its activation as a transcription factor. If expressed prematurely in meiosis, Ndt80 accumulates initially in an unmodified form that is subsequently modified by phosphorylation. In contrast, Ndt80 expressed in ime2 mutant strains does not become modified and has a reduced ability to activate transcription of its target genes. Ime2 can also phosphorylate Ndt80 in vitro, further supporting a direct role for Ime2 in the phosphorylation of Ndt80. These data indicate that Ime2 plays a novel and previously unexpected role in promoting chromosome dissemination and progress through meiotic development by activating Ndt80.

* Corresponding author. Mailing address: Department of Biochemistry, 561 Medical Sciences Building, University of Alberta, Edmonton, Alberta, Canada T6G 2H7. Phone: (780) 492-7737. Fax: (780) 492-0886. E-mail: dtstuart{at}gpu.srv.ualberta.ca.

{dagger} Present address: Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada.

Molecular and Cellular Biology, October 2002, p. 7024-7040, Vol. 22, No. 20
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.20.7024-7040.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ahmed, N. T., Bungard, D., Shin, M. E., Moore, M., Winter, E. (2009). The Ime2 Protein Kinase Enhances the Disassociation of the Sum1 Repressor from Middle Meiotic Promoters. Mol. Cell. Biol. 29: 4352-4362 [Abstract] [Full Text]  
  • McDonald, C. M., Wagner, M., Dunham, M. J., Shin, M. E., Ahmed, N. T., Winter, E. (2009). The Ras/cAMP Pathway and the CDK-Like Kinase Ime2 Regulate the MAPK Smk1 and Spore Morphogenesis in Saccharomyces cerevisiae. Genetics 181: 511-523 [Abstract] [Full Text]  
  • Lo, H.-C., Wan, L., Rosebrock, A., Futcher, B., Hollingsworth, N. M. (2008). Cdc7-Dbf4 Regulates NDT80 Transcription as Well as Reductional Segregation during Budding Yeast Meiosis. Mol. Biol. Cell 19: 4956-4967 [Abstract] [Full Text]  
  • Wan, L., Niu, H., Futcher, B., Zhang, C., Shokat, K. M., Boulton, S. J., Hollingsworth, N. M. (2008). Cdc28-Clb5 (CDK-S) and Cdc7-Dbf4 (DDK) collaborate to initiate meiotic recombination in yeast. Genes Dev. 22: 386-397 [Abstract] [Full Text]  
  • Schindler, K., Winter, E. (2006). Phosphorylation of Ime2 Regulates Meiotic Progression in Saccharomyces cerevisiae. J. Biol. Chem. 281: 18307-18316 [Abstract] [Full Text]  
  • Raithatha, S. A., Stuart, D. T. (2005). Meiosis-Specific Regulation of the Saccharomyces cerevisiae S-Phase Cyclin CLB5 Is Dependent on MluI Cell Cycle Box (MCB) Elements in Its Promoter but Is Independent of MCB-Binding Factor Activity. Genetics 169: 1329-1342 [Abstract] [Full Text]  
  • Purnapatre, K., Gray, M., Piccirillo, S., Honigberg, S. M. (2005). Glucose Inhibits Meiotic DNA Replication through SCFGrr1p-Dependent Destruction of Ime2p Kinase. Mol. Cell. Biol. 25: 440-450 [Abstract] [Full Text]  
  • Garrido, E., Voss, U., Muller, P., Castillo-Lluva, S., Kahmann, R., Perez-Martin, J. (2004). The induction of sexual development and virulence in the smut fungus Ustilago maydis depends on Crk1, a novel MAPK protein. Genes Dev. 18: 3117-3130 [Abstract] [Full Text]  
  • Fingerman, I. M., Sutphen, K., Montano, S. P., Georgiadis, M. M., Vershon, A. K. (2004). Characterization of critical interactions between Ndt80 and MSE DNA defining a novel family of Ig-fold transcription factors. Nucleic Acids Res 32: 2947-2956 [Abstract] [Full Text]  
  • Clifford, D. M., Marinco, S. M., Brush, G. S. (2004). The Meiosis-specific Protein Kinase Ime2 Directs Phosphorylation of Replication Protein A. J. Biol. Chem. 279: 6163-6170 [Abstract] [Full Text]  
  • Schindler, K., Benjamin, K. R., Martin, A., Boglioli, A., Herskowitz, I., Winter, E. (2003). The Cdk-Activating Kinase Cak1p Promotes Meiotic S Phase through Ime2p. Mol. Cell. Biol. 23: 8718-8728 [Abstract] [Full Text]  
  • Pierce, M., Benjamin, K. R., Montano, S. P., Georgiadis, M. M., Winter, E., Vershon, A. K. (2003). Sum1 and Ndt80 Proteins Compete for Binding to Middle Sporulation Element Sequences That Control Meiotic Gene Expression. Mol. Cell. Biol. 23: 4814-4825 [Abstract] [Full Text]  
  • Benjamin, K. R., Zhang, C., Shokat, K. M., Herskowitz, I. (2003). Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2. Genes Dev. 17: 1524-1539 [Abstract] [Full Text]  
  • Honigberg, S. M., Purnapatre, K. (2003). Signal pathway integration in the switch from the mitotic cell cycle to meiosis in yeast. J. Cell Sci. 116: 2137-2147 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»