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Molecular and Cellular Biology, November 2002, p. 7491-7500, Vol. 22, No. 21
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.21.7491-7500.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification, cDNA Cloning, and Targeted Deletion of p70, a Novel, Ubiquitously Expressed SH3 Domain-Containing Protein

Nick Carpino,1 Ryuji Kobayashi,2,{dagger} Heesuk Zang,1,3 Yutaka Takahashi,1 Shiann-Tarrng Jou,1,{ddagger} Jian Feng,1 Hideaki Nakajima,1,3 and James N. Ihle1,4*

Department of Biochemistry,1 Howard Hughes Medical Institute, St. Jude Children's Research Hospital,4 Department of Biochemistry, University of Tennessee Health Science Center, Memphis, Tennessee 38105,3 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 117242

Received 7 May 2002/ Returned for modification 15 July 2002/ Accepted 2 August 2002

In a screen for proteins that interact with Jak2, we identified a previously uncharacterized 70-kDa protein and cloned the corresponding cDNA. The predicated sequence indicates that p70 contains an SH3 domain and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. p70 transcripts were found in all tissues examined. Similarly, when an antibody raised against a C-terminal peptide to analyze p70 protein expression was used, all murine tissues examined were found to express p70. To investigate the in vivo role of p70, we generated a p70-deficient mouse strain. Mice lacking p70 are viable, develop normally, and do not display any obvious abnormalities. No differences were detected in various hematological parameters, including bone marrow colony-forming ability, in response to cytokines that utilize Jak2. In addition, no impairment in B- and T-cell development and proliferative ability was detected.


* Corresponding author. Mailing address: Howard Hughes Medical Institute, St. Jude Children's Research Hospital, Memphis, TN 38105. Phone: (901) 495-3422. Fax: (901) 525-8025. E-mail: james.ihle{at}stjude.org.

{dagger} Present address: Department of Molecular Pathology, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030.

{ddagger} Present address: Department of Pediatrics, National Taiwan University Hospital, Taipei 100, Taiwan.

§ Present address: Division of Cellular Therapy, The Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.


Molecular and Cellular Biology, November 2002, p. 7491-7500, Vol. 22, No. 21
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.21.7491-7500.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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