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Molecular and Cellular Biology, November 2002, p. 7543-7552, Vol. 22, No. 21
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.21.7543-7552.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

FCP1, a Phosphatase Specific for the Heptapeptide Repeat of the Largest Subunit of RNA Polymerase II, Stimulates Transcription Elongation

Subhrangsu S. Mandal, Helen Cho, Sungjoon Kim, Kettly Cabane, and Danny Reinberg^*

Division of Nucleic Acids Enzymology, Department of Biochemistry, Howard Hughes Medical Institute, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854

Received 15 May 2002/ Accepted 25 July 2002

FCP1, a phosphatase specific for the carboxy-terminal domain of RNA polymerase II (RNAP II), was found to stimulate transcript elongation by RNAP II in vitro and in vivo. This activity is independent of and distinct from the elongation-stimulatory activity associated with transcription factor IIF (TFIIF), and the elongation effects of TFIIF and FCP1 were found to be additive. Genetic experiments resulted in the isolation of several distinct fcp1 alleles. One of these alleles was found to suppress the slow-growth phenotype associated with either the reduction of intracellular nucleotide concentrations or the inhibition of other transcription elongation factors. Importantly, this allele of fcp1 was found to be lethal when combined individually with two mutations in the second-largest subunit of RNAP II, which had been shown previously to affect transcription elongation.

Present address: The Salk Institute, La Jolla, CA 92037.

Present address: The Genomics Institute of Novartis Foundation, San Diego, CA 92121.

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