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Molecular and Cellular Biology, November 2002, p. 7603-7613, Vol. 22, No. 21
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.21.7603-7613.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Targeted Disruption of NFATc3, but Not NFATc4, Reveals an Intrinsic Defect in Calcineurin-Mediated Cardiac Hypertrophic Growth

Benjamin J. Wilkins,1 Leon J. De Windt,1,{dagger} Orlando F. Bueno,1 Julian C. Braz,1 Betty J. Glascock,2 Thomas F. Kimball,2 and Jeffery D. Molkentin1*

Division of Molecular Cardiovascular Biology,1 Cardiology Division, Department of Pediatrics, Children's Hospital Medical Center, Cincinnati Ohio 45229-30392

Received 26 April 2002/ Returned for modification 3 June 2002/ Accepted 24 July 2002

A calcineurin-nuclear factor of activated T cells (NFAT) regulatory pathway has been implicated in the control of cardiac hypertrophy, suggesting one mechanism whereby alterations in intracellular calcium handling are linked to the expression of hypertrophy-associated genes. Although recent studies have demonstrated a necessary role for calcineurin as a mediator of cardiac hypertrophy, the potential involvement of NFAT transcription factors as downstream effectors of calcineurin signaling has not been evaluated. Accordingly, mice with targeted disruptions in NFATc3 and NFATc4 genes were characterized. Whereas the loss of NFATc4 did not compromise the ability of the myocardium to undergo hypertrophic growth, NFATc3-null mice demonstrated a significant reduction in calcineurin transgene-induced cardiac hypertrophy at 19 days, 26 days, 6 weeks, 8 weeks, and 10 weeks of age. NFATc3-null mice also demonstrated attenuated pressure overload- and angiotensin II-induced cardiac hypertrophy. These results provide genetic evidence that calcineurin-regulated responses require NFAT effectors in vivo.

* Corresponding author. Mailing address: Division of Molecular Cardiovascular Biology, Department of Pediatrics, Children's Hospital Medical Center, 3333 Burnet Ave., Cincinnati, OH 45229-3039. Phone: (513) 636-3557. Fax: (513) 636-5958. E-mail: molkj0{at}chmcc.org.

{dagger} Present address: Department of Cardiology, University Hospital Maastricht, Maastricht, The Netherlands.

Molecular and Cellular Biology, November 2002, p. 7603-7613, Vol. 22, No. 21
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.21.7603-7613.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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