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Molecular and Cellular Biology, December 2002, p. 8114-8121, Vol. 22, No. 23
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.23.8114-8121.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of a Human Decapping Complex Associated with hUpf Proteins in Nonsense-Mediated Decay

Jens Lykke-Andersen*

Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309

Received 19 July 2002/ Returned for modification 19 August 2002/ Accepted 10 September 2002

Decapping is a key step in general and regulated mRNA decay. In Saccharomyces cerevisiae it constitutes a rate-limiting step in the nonsense-mediated decay pathway that rids cells of mRNAs containing premature termination codons. Here two human decapping enzymes are identified, hDcp1a and hDcp2, as well as a homolog of hDcp1a, termed hDcp1b. Transiently expressed hDcp1a and hDcp2 proteins localize primarily to the cytoplasm and form a complex in human cell extracts. hDcp1a and hDcp2 copurify with decapping activity, an activity sensitive to mutation of critical hDcp residues. Importantly, coimmunoprecipitation assays demonstrate that hDcp1a and hDcp2 interact with the nonsense-mediated decay factor hUpf1, both in the presence and in the absence of the other hUpf proteins, hUpf2, hUpf3a, and hUpf3b. These data suggest that a human decapping complex may be recruited to mRNAs containing premature termination codons by the hUpf proteins.

* Mailing address: Molecular, Cellular and Developmental Biology, University of Colorado, CB 347, Boulder, CO 80309. Phone: (303) 735-4886. Fax: (303) 492-7744. E-mail: Jens.Lykke-Andersen{at}colorado.edu.

Molecular and Cellular Biology, December 2002, p. 8114-8121, Vol. 22, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.23.8114-8121.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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