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Molecular and Cellular Biology, December 2002, p. 8241-8253, Vol. 22, No. 23
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.23.8241-8253.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Stable mRNP Formation and Export Require Cotranscriptional Recruitment of the mRNA Export Factors Yra1p and Sub2p by Hpr1p

Daniel Zenklusen, Patrizia Vinciguerra, Jean-Christophe Wyss, and Françoise Stutz*

Institute of Microbiology, University of Lausanne, 1011 Lausanne, Switzerland

Received 8 July 2002/ Returned for modification 23 August 2002/ Accepted 4 September 2002

Yra1p/REF participates in mRNA export by recruiting the export receptor Mex67p to messenger ribonucleoprotein (mRNP) complexes. Yra1p also binds Sub2p, a DEAD box ATPase/RNA helicase implicated in splicing and required for mRNA export. We identified genetic and physical interactions between Yra1p, Sub2p, and Hpr1p, a protein involved in transcription elongation whose deletion leads to poly(A)+ RNA accumulation in the nucleus. By chromatin immunoprecipitation (ChIP) experiments, we show that Hpr1p, Sub2p, and Yra1p become associated with active genes during transcription elongation and that Hpr1p is required for the efficient recruitment of Sub2p and Yra1p. The data indicate that transcription and export are functionally linked and that mRNA export defects may be due in part to inefficient loading of essential mRNA export factors on the growing mRNP. We also identified functional interactions between Yra1p and the exosome components Rrp45p and Rrp6p. We show that yra1, sub2, and {Delta}hpr1 mutants all present defects in mRNA accumulation and that deletion of RRP6 in yra1 mutants restores normal mRNA levels. The data support the hypothesis that an exosome-dependent surveillance mechanism targets improperly assembled mRNPs for degradation.

* Corresponding author. Mailing address: Institute of Microbiology, University of Lausanne, Bugnon 44, 1011 Lausanne, Switzerland. Phone: 41 21 314 40 84. Fax: 41 21 314 40 95. E-mail: francoise.stutz{at}chuv.hospvd.ch.

Molecular and Cellular Biology, December 2002, p. 8241-8253, Vol. 22, No. 23
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.23.8241-8253.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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