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Molecular and Cellular Biology, December 2002, p. 8415-8425, Vol. 22, No. 24
0270-7306/02/$04.00+0     DOI: 10.1128/MCB.22.24.8415-8425.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

A Sequence Element Downstream of the Yeast HTB1 Gene Contributes to mRNA 3' Processing and Cell Cycle Regulation

Susan G. Campbell,¹ Marcel li del Olmo,² Paul Beglan,¹ and Ursula Bond^1*

Microbiology Department, Moyne Institute for Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland,¹ Departament de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, and Departamento de Biotecnología, Instituto de Agroquímica y tecnología de Alimentos (IATA), M. L. Burjassot, Spain²

Received 17 July 2002/ Accepted 17 September 2002

Histone mRNAs accumulate in the S phase and are rapidly degraded as cells progress into the G₂ phase of the cell cycle. In Saccharomyces cerevisiae, fusion of the 3' untranslated region and downstream sequences of the yeast histone gene HTB1 to a neomycin phosphotransferase open reading frame is sufficient to confer cell cycle regulation on the resulting chimera gene (neo-HTB1). We have identified a sequence element, designated the distal downstream element (DDE), that influences both the 3'-end cleavage site selection and the cell cycle regulation of the neo-HTB1 mRNA. Mutations in the DDE, which is located approximately 110 nucleotides downstream of the HTB1 gene, lead to a delay in the accumulation of the neo-HTB1 mRNA in the S phase and a lack of mRNA turnover in the G₂ phase. The DDE is transcribed as part of the primary transcript and binds a protein factor(s). Maximum binding is observed in the S phase of the cell cycle, and mutations that affect the turnover of the HTB1 mRNA alter the binding activity. While located in the same general region, mutations that affect 3'-end cleavage site selection act independently from those that alter the cell cycle regulation.

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* Corresponding author. Mailing address: Microbiology Department, Moyne Institute for Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland. Phone: 353-1-608-2578. Fax: 353-1-679-9294. E-mail: ubond{at}tcd.ie.

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Molecular and Cellular Biology, December 2002, p. 8415-8425, Vol. 22, No. 24
0022-538X/02/$04.00+0     DOI: 10.1128/MCB.22.24.8415-8425.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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